Engineered dual selection for directed evolution of SpCas9 PAM specificity

The PAM specificity of SpCas9 can be altered with positive selection during directed evolution. Here the authors use simultaneous positive and negative selection to improve activity on NAG PAMs while reducing activity on NGG PAMs.

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Autores principales:	Gregory W. Goldberg, Jeffrey M. Spencer, David O. Giganti, Brendan R. Camellato, Neta Agmon, David M. Ichikawa, Jef D. Boeke, Marcus B. Noyes
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Science Q
Acceso en línea:	https://doaj.org/article/3e675de09c284972a7413a9b4147d955
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spelling	oai:doaj.org-article:3e675de09c284972a7413a9b4147d9552021-12-02T14:11:57ZEngineered dual selection for directed evolution of SpCas9 PAM specificity10.1038/s41467-020-20650-x2041-1723https://doaj.org/article/3e675de09c284972a7413a9b4147d9552021-01-01T00:00:00Zhttps://doi.org/10.1038/s41467-020-20650-xhttps://doaj.org/toc/2041-1723The PAM specificity of SpCas9 can be altered with positive selection during directed evolution. Here the authors use simultaneous positive and negative selection to improve activity on NAG PAMs while reducing activity on NGG PAMs.Gregory W. GoldbergJeffrey M. SpencerDavid O. GigantiBrendan R. CamellatoNeta AgmonDavid M. IchikawaJef D. BoekeMarcus B. NoyesNature PortfolioarticleScienceQENNature Communications, Vol 12, Iss 1, Pp 1-16 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q Gregory W. Goldberg Jeffrey M. Spencer David O. Giganti Brendan R. Camellato Neta Agmon David M. Ichikawa Jef D. Boeke Marcus B. Noyes Engineered dual selection for directed evolution of SpCas9 PAM specificity
description	The PAM specificity of SpCas9 can be altered with positive selection during directed evolution. Here the authors use simultaneous positive and negative selection to improve activity on NAG PAMs while reducing activity on NGG PAMs.
format	article
author	Gregory W. Goldberg Jeffrey M. Spencer David O. Giganti Brendan R. Camellato Neta Agmon David M. Ichikawa Jef D. Boeke Marcus B. Noyes
author_facet	Gregory W. Goldberg Jeffrey M. Spencer David O. Giganti Brendan R. Camellato Neta Agmon David M. Ichikawa Jef D. Boeke Marcus B. Noyes
author_sort	Gregory W. Goldberg
title	Engineered dual selection for directed evolution of SpCas9 PAM specificity
title_short	Engineered dual selection for directed evolution of SpCas9 PAM specificity
title_full	Engineered dual selection for directed evolution of SpCas9 PAM specificity
title_fullStr	Engineered dual selection for directed evolution of SpCas9 PAM specificity
title_full_unstemmed	Engineered dual selection for directed evolution of SpCas9 PAM specificity
title_sort	engineered dual selection for directed evolution of spcas9 pam specificity
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/3e675de09c284972a7413a9b4147d955
work_keys_str_mv	AT gregorywgoldberg engineereddualselectionfordirectedevolutionofspcas9pamspecificity AT jeffreymspencer engineereddualselectionfordirectedevolutionofspcas9pamspecificity AT davidogiganti engineereddualselectionfordirectedevolutionofspcas9pamspecificity AT brendanrcamellato engineereddualselectionfordirectedevolutionofspcas9pamspecificity AT netaagmon engineereddualselectionfordirectedevolutionofspcas9pamspecificity AT davidmichikawa engineereddualselectionfordirectedevolutionofspcas9pamspecificity AT jefdboeke engineereddualselectionfordirectedevolutionofspcas9pamspecificity AT marcusbnoyes engineereddualselectionfordirectedevolutionofspcas9pamspecificity
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Engineered dual selection for directed evolution of SpCas9 PAM specificity

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