Selective N-terminal acylation of peptides and proteins with a Gly-His tag sequence

His-tagged proteins can undergo N-terminal acylation as an undesired side-reaction. Here, the authors utilize this to develop a method for highly selective acylation and further modification of peptides and proteins using an optimized His sequence and 4-methoxyphenyl esters as acyl donors.

Guardado en:

Detalles Bibliográficos
Autores principales:	Manuel C. Martos-Maldonado, Christian T. Hjuler, Kasper K. Sørensen, Mikkel B. Thygesen, Jakob E. Rasmussen, Klaus Villadsen, Søren R. Midtgaard, Stefan Kol, Sanne Schoffelen, Knud J. Jensen
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2018
Materias:	Science Q
Acceso en línea:	https://doaj.org/article/3e78d4a3733a4cc3b314ad992f024827
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:3e78d4a3733a4cc3b314ad992f024827
record_format	dspace
spelling	oai:doaj.org-article:3e78d4a3733a4cc3b314ad992f0248272021-12-02T17:32:15ZSelective N-terminal acylation of peptides and proteins with a Gly-His tag sequence10.1038/s41467-018-05695-32041-1723https://doaj.org/article/3e78d4a3733a4cc3b314ad992f0248272018-08-01T00:00:00Zhttps://doi.org/10.1038/s41467-018-05695-3https://doaj.org/toc/2041-1723His-tagged proteins can undergo N-terminal acylation as an undesired side-reaction. Here, the authors utilize this to develop a method for highly selective acylation and further modification of peptides and proteins using an optimized His sequence and 4-methoxyphenyl esters as acyl donors.Manuel C. Martos-MaldonadoChristian T. HjulerKasper K. SørensenMikkel B. ThygesenJakob E. RasmussenKlaus VilladsenSøren R. MidtgaardStefan KolSanne SchoffelenKnud J. JensenNature PortfolioarticleScienceQENNature Communications, Vol 9, Iss 1, Pp 1-13 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q Manuel C. Martos-Maldonado Christian T. Hjuler Kasper K. Sørensen Mikkel B. Thygesen Jakob E. Rasmussen Klaus Villadsen Søren R. Midtgaard Stefan Kol Sanne Schoffelen Knud J. Jensen Selective N-terminal acylation of peptides and proteins with a Gly-His tag sequence
description	His-tagged proteins can undergo N-terminal acylation as an undesired side-reaction. Here, the authors utilize this to develop a method for highly selective acylation and further modification of peptides and proteins using an optimized His sequence and 4-methoxyphenyl esters as acyl donors.
format	article
author	Manuel C. Martos-Maldonado Christian T. Hjuler Kasper K. Sørensen Mikkel B. Thygesen Jakob E. Rasmussen Klaus Villadsen Søren R. Midtgaard Stefan Kol Sanne Schoffelen Knud J. Jensen
author_facet	Manuel C. Martos-Maldonado Christian T. Hjuler Kasper K. Sørensen Mikkel B. Thygesen Jakob E. Rasmussen Klaus Villadsen Søren R. Midtgaard Stefan Kol Sanne Schoffelen Knud J. Jensen
author_sort	Manuel C. Martos-Maldonado
title	Selective N-terminal acylation of peptides and proteins with a Gly-His tag sequence
title_short	Selective N-terminal acylation of peptides and proteins with a Gly-His tag sequence
title_full	Selective N-terminal acylation of peptides and proteins with a Gly-His tag sequence
title_fullStr	Selective N-terminal acylation of peptides and proteins with a Gly-His tag sequence
title_full_unstemmed	Selective N-terminal acylation of peptides and proteins with a Gly-His tag sequence
title_sort	selective n-terminal acylation of peptides and proteins with a gly-his tag sequence
publisher	Nature Portfolio
publishDate	2018
url	https://doaj.org/article/3e78d4a3733a4cc3b314ad992f024827
work_keys_str_mv	AT manuelcmartosmaldonado selectiventerminalacylationofpeptidesandproteinswithaglyhistagsequence AT christianthjuler selectiventerminalacylationofpeptidesandproteinswithaglyhistagsequence AT kasperksørensen selectiventerminalacylationofpeptidesandproteinswithaglyhistagsequence AT mikkelbthygesen selectiventerminalacylationofpeptidesandproteinswithaglyhistagsequence AT jakoberasmussen selectiventerminalacylationofpeptidesandproteinswithaglyhistagsequence AT klausvilladsen selectiventerminalacylationofpeptidesandproteinswithaglyhistagsequence AT sørenrmidtgaard selectiventerminalacylationofpeptidesandproteinswithaglyhistagsequence AT stefankol selectiventerminalacylationofpeptidesandproteinswithaglyhistagsequence AT sanneschoffelen selectiventerminalacylationofpeptidesandproteinswithaglyhistagsequence AT knudjjensen selectiventerminalacylationofpeptidesandproteinswithaglyhistagsequence
_version_	1718380362490445824

Selective N-terminal acylation of peptides and proteins with a Gly-His tag sequence

Ejemplares similares