The Basic Helix-Loop-Helix Transcription Factor SmbHLH1 Represses Anthocyanin Biosynthesis in Eggplant
Many basic helix-loop-helix transcription factors (TFs) have been reported to promote anthocyanin biosynthesis in numerous plant species, but little is known about bHLH TFs that inhibit anthocyanin accumulation. In this study, SmbHLH1 from Solanum melongena was identified as a negative regulator of...
Guardado en:
| Autores principales: | , , , , , |
|---|---|
| Formato: | article |
| Lenguaje: | EN |
| Publicado: |
Frontiers Media S.A.
2021
|
| Materias: | |
| Acceso en línea: | https://doaj.org/article/3e7d60ec91674f33bd71984f7d2c0cae |
| Etiquetas: |
Agregar Etiqueta
Sin Etiquetas, Sea el primero en etiquetar este registro!
|
| id |
oai:doaj.org-article:3e7d60ec91674f33bd71984f7d2c0cae |
|---|---|
| record_format |
dspace |
| spelling |
oai:doaj.org-article:3e7d60ec91674f33bd71984f7d2c0cae2021-11-12T17:28:18ZThe Basic Helix-Loop-Helix Transcription Factor SmbHLH1 Represses Anthocyanin Biosynthesis in Eggplant1664-462X10.3389/fpls.2021.757936https://doaj.org/article/3e7d60ec91674f33bd71984f7d2c0cae2021-11-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fpls.2021.757936/fullhttps://doaj.org/toc/1664-462XMany basic helix-loop-helix transcription factors (TFs) have been reported to promote anthocyanin biosynthesis in numerous plant species, but little is known about bHLH TFs that inhibit anthocyanin accumulation. In this study, SmbHLH1 from Solanum melongena was identified as a negative regulator of anthocyanin biosynthesis. However, SmbHLH1 showed high identity with SmTT8, which acts as a SmMYB113-dependent positive regulator of anthocyanin-biosynthesis in plants. Overexpression of SmbHLH1 in eggplant caused a dramatic decrease in anthocyanin accumulation. Only the amino acid sequences at the N and C termini of SmbHLH1 differed from the SmTT8 sequence. Expression analysis revealed that the expression pattern of SmbHLH1 was opposite to that of anthocyanin accumulation. Yeast two-hybrid (Y2H) and bimolecular fluorescence complementation (BiFC) assays showed that SmbHLH1 could not interact with SmMYB113. Dual-luciferase assay demonstrated that SmbHLH1 directly repressed the expression of SmDFR and SmANS. Our results demonstrate that the biological function of bHLHs in anthocyanin biosynthesis may have evolved and provide new insight into the molecular functions of orthologous genes from different plant species.Zhaofei DuanShiyu TianGuobin YangMin WeiMin WeiMin WeiJing LiJing LiJing LiFengjuan YangFengjuan YangFengjuan YangFrontiers Media S.A.articleeggplantanthocyanin biosynthesisbHLH transcription factornegative regulationRNA-seqPlant cultureSB1-1110ENFrontiers in Plant Science, Vol 12 (2021) |
| institution |
DOAJ |
| collection |
DOAJ |
| language |
EN |
| topic |
eggplant anthocyanin biosynthesis bHLH transcription factor negative regulation RNA-seq Plant culture SB1-1110 |
| spellingShingle |
eggplant anthocyanin biosynthesis bHLH transcription factor negative regulation RNA-seq Plant culture SB1-1110 Zhaofei Duan Shiyu Tian Guobin Yang Min Wei Min Wei Min Wei Jing Li Jing Li Jing Li Fengjuan Yang Fengjuan Yang Fengjuan Yang The Basic Helix-Loop-Helix Transcription Factor SmbHLH1 Represses Anthocyanin Biosynthesis in Eggplant |
| description |
Many basic helix-loop-helix transcription factors (TFs) have been reported to promote anthocyanin biosynthesis in numerous plant species, but little is known about bHLH TFs that inhibit anthocyanin accumulation. In this study, SmbHLH1 from Solanum melongena was identified as a negative regulator of anthocyanin biosynthesis. However, SmbHLH1 showed high identity with SmTT8, which acts as a SmMYB113-dependent positive regulator of anthocyanin-biosynthesis in plants. Overexpression of SmbHLH1 in eggplant caused a dramatic decrease in anthocyanin accumulation. Only the amino acid sequences at the N and C termini of SmbHLH1 differed from the SmTT8 sequence. Expression analysis revealed that the expression pattern of SmbHLH1 was opposite to that of anthocyanin accumulation. Yeast two-hybrid (Y2H) and bimolecular fluorescence complementation (BiFC) assays showed that SmbHLH1 could not interact with SmMYB113. Dual-luciferase assay demonstrated that SmbHLH1 directly repressed the expression of SmDFR and SmANS. Our results demonstrate that the biological function of bHLHs in anthocyanin biosynthesis may have evolved and provide new insight into the molecular functions of orthologous genes from different plant species. |
| format |
article |
| author |
Zhaofei Duan Shiyu Tian Guobin Yang Min Wei Min Wei Min Wei Jing Li Jing Li Jing Li Fengjuan Yang Fengjuan Yang Fengjuan Yang |
| author_facet |
Zhaofei Duan Shiyu Tian Guobin Yang Min Wei Min Wei Min Wei Jing Li Jing Li Jing Li Fengjuan Yang Fengjuan Yang Fengjuan Yang |
| author_sort |
Zhaofei Duan |
| title |
The Basic Helix-Loop-Helix Transcription Factor SmbHLH1 Represses Anthocyanin Biosynthesis in Eggplant |
| title_short |
The Basic Helix-Loop-Helix Transcription Factor SmbHLH1 Represses Anthocyanin Biosynthesis in Eggplant |
| title_full |
The Basic Helix-Loop-Helix Transcription Factor SmbHLH1 Represses Anthocyanin Biosynthesis in Eggplant |
| title_fullStr |
The Basic Helix-Loop-Helix Transcription Factor SmbHLH1 Represses Anthocyanin Biosynthesis in Eggplant |
| title_full_unstemmed |
The Basic Helix-Loop-Helix Transcription Factor SmbHLH1 Represses Anthocyanin Biosynthesis in Eggplant |
| title_sort |
basic helix-loop-helix transcription factor smbhlh1 represses anthocyanin biosynthesis in eggplant |
| publisher |
Frontiers Media S.A. |
| publishDate |
2021 |
| url |
https://doaj.org/article/3e7d60ec91674f33bd71984f7d2c0cae |
| work_keys_str_mv |
AT zhaofeiduan thebasichelixloophelixtranscriptionfactorsmbhlh1repressesanthocyaninbiosynthesisineggplant AT shiyutian thebasichelixloophelixtranscriptionfactorsmbhlh1repressesanthocyaninbiosynthesisineggplant AT guobinyang thebasichelixloophelixtranscriptionfactorsmbhlh1repressesanthocyaninbiosynthesisineggplant AT minwei thebasichelixloophelixtranscriptionfactorsmbhlh1repressesanthocyaninbiosynthesisineggplant AT minwei thebasichelixloophelixtranscriptionfactorsmbhlh1repressesanthocyaninbiosynthesisineggplant AT minwei thebasichelixloophelixtranscriptionfactorsmbhlh1repressesanthocyaninbiosynthesisineggplant AT jingli thebasichelixloophelixtranscriptionfactorsmbhlh1repressesanthocyaninbiosynthesisineggplant AT jingli thebasichelixloophelixtranscriptionfactorsmbhlh1repressesanthocyaninbiosynthesisineggplant AT jingli thebasichelixloophelixtranscriptionfactorsmbhlh1repressesanthocyaninbiosynthesisineggplant AT fengjuanyang thebasichelixloophelixtranscriptionfactorsmbhlh1repressesanthocyaninbiosynthesisineggplant AT fengjuanyang thebasichelixloophelixtranscriptionfactorsmbhlh1repressesanthocyaninbiosynthesisineggplant AT fengjuanyang thebasichelixloophelixtranscriptionfactorsmbhlh1repressesanthocyaninbiosynthesisineggplant AT zhaofeiduan basichelixloophelixtranscriptionfactorsmbhlh1repressesanthocyaninbiosynthesisineggplant AT shiyutian basichelixloophelixtranscriptionfactorsmbhlh1repressesanthocyaninbiosynthesisineggplant AT guobinyang basichelixloophelixtranscriptionfactorsmbhlh1repressesanthocyaninbiosynthesisineggplant AT minwei basichelixloophelixtranscriptionfactorsmbhlh1repressesanthocyaninbiosynthesisineggplant AT minwei basichelixloophelixtranscriptionfactorsmbhlh1repressesanthocyaninbiosynthesisineggplant AT minwei basichelixloophelixtranscriptionfactorsmbhlh1repressesanthocyaninbiosynthesisineggplant AT jingli basichelixloophelixtranscriptionfactorsmbhlh1repressesanthocyaninbiosynthesisineggplant AT jingli basichelixloophelixtranscriptionfactorsmbhlh1repressesanthocyaninbiosynthesisineggplant AT jingli basichelixloophelixtranscriptionfactorsmbhlh1repressesanthocyaninbiosynthesisineggplant AT fengjuanyang basichelixloophelixtranscriptionfactorsmbhlh1repressesanthocyaninbiosynthesisineggplant AT fengjuanyang basichelixloophelixtranscriptionfactorsmbhlh1repressesanthocyaninbiosynthesisineggplant AT fengjuanyang basichelixloophelixtranscriptionfactorsmbhlh1repressesanthocyaninbiosynthesisineggplant |
| _version_ |
1718430310010454016 |