Connection of core and tail Mediator modules restrains transcription from TFIID-dependent promoters.

The Mediator coactivator complex is divided into four modules: head, middle, tail, and kinase. Deletion of the architectural subunit Med16 separates core Mediator (cMed), comprising the head, middle, and scaffold (Med14), from the tail. However, the direct global effects of tail/cMed disconnection a...

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Autores principales:	Moustafa M Saleh, Célia Jeronimo, François Robert, Gabriel E Zentner
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2021
Materias:	Genetics QH426-470
Acceso en línea:	https://doaj.org/article/3e81cd6f09894d228e05539d192e6b5c
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spelling	oai:doaj.org-article:3e81cd6f09894d228e05539d192e6b5c2021-12-02T20:03:23ZConnection of core and tail Mediator modules restrains transcription from TFIID-dependent promoters.1553-73901553-740410.1371/journal.pgen.1009529https://doaj.org/article/3e81cd6f09894d228e05539d192e6b5c2021-08-01T00:00:00Zhttps://doi.org/10.1371/journal.pgen.1009529https://doaj.org/toc/1553-7390https://doaj.org/toc/1553-7404The Mediator coactivator complex is divided into four modules: head, middle, tail, and kinase. Deletion of the architectural subunit Med16 separates core Mediator (cMed), comprising the head, middle, and scaffold (Med14), from the tail. However, the direct global effects of tail/cMed disconnection are unclear. We find that rapid depletion of Med16 downregulates genes that require the SAGA complex for full expression, consistent with their reported tail dependence, but also moderately overactivates TFIID-dependent genes in a manner partly dependent on the separated tail, which remains associated with upstream activating sequences. Suppression of TBP dynamics via removal of the Mot1 ATPase partially restores normal transcriptional activity to Med16-depleted cells, suggesting that cMed/tail separation results in an imbalance in the levels of PIC formation at SAGA-requiring and TFIID-dependent genes. We propose that the preferential regulation of SAGA-requiring genes by tailed Mediator helps maintain a proper balance of transcription between these genes and those more dependent on TFIID.Moustafa M SalehCélia JeronimoFrançois RobertGabriel E ZentnerPublic Library of Science (PLoS)articleGeneticsQH426-470ENPLoS Genetics, Vol 17, Iss 8, p e1009529 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Genetics QH426-470
spellingShingle	Genetics QH426-470 Moustafa M Saleh Célia Jeronimo François Robert Gabriel E Zentner Connection of core and tail Mediator modules restrains transcription from TFIID-dependent promoters.
description	The Mediator coactivator complex is divided into four modules: head, middle, tail, and kinase. Deletion of the architectural subunit Med16 separates core Mediator (cMed), comprising the head, middle, and scaffold (Med14), from the tail. However, the direct global effects of tail/cMed disconnection are unclear. We find that rapid depletion of Med16 downregulates genes that require the SAGA complex for full expression, consistent with their reported tail dependence, but also moderately overactivates TFIID-dependent genes in a manner partly dependent on the separated tail, which remains associated with upstream activating sequences. Suppression of TBP dynamics via removal of the Mot1 ATPase partially restores normal transcriptional activity to Med16-depleted cells, suggesting that cMed/tail separation results in an imbalance in the levels of PIC formation at SAGA-requiring and TFIID-dependent genes. We propose that the preferential regulation of SAGA-requiring genes by tailed Mediator helps maintain a proper balance of transcription between these genes and those more dependent on TFIID.
format	article
author	Moustafa M Saleh Célia Jeronimo François Robert Gabriel E Zentner
author_facet	Moustafa M Saleh Célia Jeronimo François Robert Gabriel E Zentner
author_sort	Moustafa M Saleh
title	Connection of core and tail Mediator modules restrains transcription from TFIID-dependent promoters.
title_short	Connection of core and tail Mediator modules restrains transcription from TFIID-dependent promoters.
title_full	Connection of core and tail Mediator modules restrains transcription from TFIID-dependent promoters.
title_fullStr	Connection of core and tail Mediator modules restrains transcription from TFIID-dependent promoters.
title_full_unstemmed	Connection of core and tail Mediator modules restrains transcription from TFIID-dependent promoters.
title_sort	connection of core and tail mediator modules restrains transcription from tfiid-dependent promoters.
publisher	Public Library of Science (PLoS)
publishDate	2021
url	https://doaj.org/article/3e81cd6f09894d228e05539d192e6b5c
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_version_	1718375653971066880

Connection of core and tail Mediator modules restrains transcription from TFIID-dependent promoters.

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