Updating recommendations of the Brazilian Group of Flow Cytometry (GBCFLUX) for diagnosis of acute leukemias using four-color flow cytometry panels

Introduction: Flow cytometry has become an increasingly important tool in the clinical laboratory for the diagnosis and monitoring of many hematopoietic neoplasms. This method is ideal for immunophenotypic identification of cellular subpopulations in complex samples, such as bone marrow and peripher...

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Autores principales:	Míriam P. Beltrame, Elizabeth Xisto Souto, Mihoko Yamamoto, Felipe M Furtado, Elaine Sobral da Costa, Alex Freire Sandes, Glicínia Pimenta, Geraldo Barroso Cavalcanti Júnior, Maria Cláudia Santos-Silva, Irene Lorand-Metze, Maura R V Ikoma-Colturato
Formato:	article
Lenguaje:	EN
Publicado:	Elsevier 2021
Materias:	Flow cytometry Acute leukemia panel Monoclonal antibodies panel ALL AML GBCFLUX Diseases of the blood and blood-forming organs RC633-647.5
Acceso en línea:	https://doaj.org/article/3e81e384c5e1424884a4fa855d4648a4
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spelling	oai:doaj.org-article:3e81e384c5e1424884a4fa855d4648a42021-11-04T04:37:37ZUpdating recommendations of the Brazilian Group of Flow Cytometry (GBCFLUX) for diagnosis of acute leukemias using four-color flow cytometry panels2531-137910.1016/j.htct.2021.04.001https://doaj.org/article/3e81e384c5e1424884a4fa855d4648a42021-10-01T00:00:00Zhttp://www.sciencedirect.com/science/article/pii/S2531137921000602https://doaj.org/toc/2531-1379Introduction: Flow cytometry has become an increasingly important tool in the clinical laboratory for the diagnosis and monitoring of many hematopoietic neoplasms. This method is ideal for immunophenotypic identification of cellular subpopulations in complex samples, such as bone marrow and peripheral blood. In general, 4-color panels appear to be adequate, depending on the assay. In acute leukemias (ALs), it is necessary identify and characterize the population of abnormal cells in order to recognize the compromised lineage and classify leukemia according to the WHO criteria. Although the use of eight- to ten-color immunophenotyping panels is wellestablished, many laboratories do not have access to this technology. Objective and Method: In 2015, the Brazilian Group of Flow Cytometry (Grupo Brasileiro de Citometria de Fluxo, GBCFLUX) proposed antibody panels designed to allow the precise diagnosis and characterization of AL within available resources. As many Brazilian flow cytometry laboratories use four-color immunophenotyping, the GBCFLUX has updated that document, according to current leukemia knowledge and after a forum of discussion and validation of antibody panels. Results: Recommendations for morphological analysis of bone marrow smears and performing screening panel for lineage (s) identification of AL were maintained from the previous publication. The lineage-oriented proposed panels for B and T cell acute lymphoblastic leukemia (ALL) and for acute myeloid leukemia (AML) were constructed for an appropriate leukemia classification. Conclusion: Three levels of recommendations (i.e., mandatory, recommended, and optional) were established to enable an accurate diagnosis with some flexibility, considering local laboratory resources and patient-specific needs.Míriam P. BeltrameElizabeth Xisto SoutoMihoko YamamotoFelipe M FurtadoElaine Sobral da CostaAlex Freire SandesGlicínia PimentaGeraldo Barroso Cavalcanti JúniorMaria Cláudia Santos-SilvaIrene Lorand-MetzeMaura R V Ikoma-ColturatoElsevierarticleFlow cytometryAcute leukemia panelMonoclonal antibodies panelALLAMLGBCFLUXDiseases of the blood and blood-forming organsRC633-647.5ENHematology, Transfusion and Cell Therapy, Vol 43, Iss 4, Pp 499-506 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Flow cytometry Acute leukemia panel Monoclonal antibodies panel ALL AML GBCFLUX Diseases of the blood and blood-forming organs RC633-647.5
spellingShingle	Flow cytometry Acute leukemia panel Monoclonal antibodies panel ALL AML GBCFLUX Diseases of the blood and blood-forming organs RC633-647.5 Míriam P. Beltrame Elizabeth Xisto Souto Mihoko Yamamoto Felipe M Furtado Elaine Sobral da Costa Alex Freire Sandes Glicínia Pimenta Geraldo Barroso Cavalcanti Júnior Maria Cláudia Santos-Silva Irene Lorand-Metze Maura R V Ikoma-Colturato Updating recommendations of the Brazilian Group of Flow Cytometry (GBCFLUX) for diagnosis of acute leukemias using four-color flow cytometry panels
description	Introduction: Flow cytometry has become an increasingly important tool in the clinical laboratory for the diagnosis and monitoring of many hematopoietic neoplasms. This method is ideal for immunophenotypic identification of cellular subpopulations in complex samples, such as bone marrow and peripheral blood. In general, 4-color panels appear to be adequate, depending on the assay. In acute leukemias (ALs), it is necessary identify and characterize the population of abnormal cells in order to recognize the compromised lineage and classify leukemia according to the WHO criteria. Although the use of eight- to ten-color immunophenotyping panels is wellestablished, many laboratories do not have access to this technology. Objective and Method: In 2015, the Brazilian Group of Flow Cytometry (Grupo Brasileiro de Citometria de Fluxo, GBCFLUX) proposed antibody panels designed to allow the precise diagnosis and characterization of AL within available resources. As many Brazilian flow cytometry laboratories use four-color immunophenotyping, the GBCFLUX has updated that document, according to current leukemia knowledge and after a forum of discussion and validation of antibody panels. Results: Recommendations for morphological analysis of bone marrow smears and performing screening panel for lineage (s) identification of AL were maintained from the previous publication. The lineage-oriented proposed panels for B and T cell acute lymphoblastic leukemia (ALL) and for acute myeloid leukemia (AML) were constructed for an appropriate leukemia classification. Conclusion: Three levels of recommendations (i.e., mandatory, recommended, and optional) were established to enable an accurate diagnosis with some flexibility, considering local laboratory resources and patient-specific needs.
format	article
author	Míriam P. Beltrame Elizabeth Xisto Souto Mihoko Yamamoto Felipe M Furtado Elaine Sobral da Costa Alex Freire Sandes Glicínia Pimenta Geraldo Barroso Cavalcanti Júnior Maria Cláudia Santos-Silva Irene Lorand-Metze Maura R V Ikoma-Colturato
author_facet	Míriam P. Beltrame Elizabeth Xisto Souto Mihoko Yamamoto Felipe M Furtado Elaine Sobral da Costa Alex Freire Sandes Glicínia Pimenta Geraldo Barroso Cavalcanti Júnior Maria Cláudia Santos-Silva Irene Lorand-Metze Maura R V Ikoma-Colturato
author_sort	Míriam P. Beltrame
title	Updating recommendations of the Brazilian Group of Flow Cytometry (GBCFLUX) for diagnosis of acute leukemias using four-color flow cytometry panels
title_short	Updating recommendations of the Brazilian Group of Flow Cytometry (GBCFLUX) for diagnosis of acute leukemias using four-color flow cytometry panels
title_full	Updating recommendations of the Brazilian Group of Flow Cytometry (GBCFLUX) for diagnosis of acute leukemias using four-color flow cytometry panels
title_fullStr	Updating recommendations of the Brazilian Group of Flow Cytometry (GBCFLUX) for diagnosis of acute leukemias using four-color flow cytometry panels
title_full_unstemmed	Updating recommendations of the Brazilian Group of Flow Cytometry (GBCFLUX) for diagnosis of acute leukemias using four-color flow cytometry panels
title_sort	updating recommendations of the brazilian group of flow cytometry (gbcflux) for diagnosis of acute leukemias using four-color flow cytometry panels
publisher	Elsevier
publishDate	2021
url	https://doaj.org/article/3e81e384c5e1424884a4fa855d4648a4
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Updating recommendations of the Brazilian Group of Flow Cytometry (GBCFLUX) for diagnosis of acute leukemias using four-color flow cytometry panels

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