Gelatin/bioactive glass composite scaffold for promoting the migration and odontogenic differentiation of bone marrow mesenchymal stem cells

Recruiting periapical stem cells into the root canal and inducing their odontogenic differentiation are both vital for pulp regeneration. This study was aimed at developing a kind of bioactive composite scaffolds (G/PSC scaffolds) by combining the pH-neutral bioactive glass named PSC and gelatin. Th...

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Autores principales:	Guibin Huang, Liju Xu, Jilin Wu, Sainan Wang, Yanmei Dong
Formato:	article
Lenguaje:	EN
Publicado:	Elsevier 2021
Materias:	pH-neutral bioactive glass Composite scaffold Bone marrow mesenchymal stem cells Migration Odontogenic differentiation Pulp regeneration Polymers and polymer manufacture TP1080-1185
Acceso en línea:	https://doaj.org/article/3e85773806e347d9afee704689bd9df2
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spelling	oai:doaj.org-article:3e85773806e347d9afee704689bd9df22021-11-24T04:24:14ZGelatin/bioactive glass composite scaffold for promoting the migration and odontogenic differentiation of bone marrow mesenchymal stem cells0142-941810.1016/j.polymertesting.2020.106915https://doaj.org/article/3e85773806e347d9afee704689bd9df22021-01-01T00:00:00Zhttp://www.sciencedirect.com/science/article/pii/S0142941820321449https://doaj.org/toc/0142-9418Recruiting periapical stem cells into the root canal and inducing their odontogenic differentiation are both vital for pulp regeneration. This study was aimed at developing a kind of bioactive composite scaffolds (G/PSC scaffolds) by combining the pH-neutral bioactive glass named PSC and gelatin. The incorporation of PSC endowed the scaffolds with bioactivity and enhanced its resistance to enzymatic hydrolysis in vitro while maintaining its high water absorption capacity. The G/PSC scaffold with a PSC content of 0.5 mg/mL in 3% gelatin solution showed the best overall performance, released large amounts of silicate ions and maintained a neutral pH after soaking in simulated body fluid (SBF). Human bone marrow mesenchymal stem cells (hBMMSCs) developed good cell morphology with affluent pseudopods on the G/PSC scaffold and infiltrated fairly deep into it. Additionally, hBMMSCs showed increased proliferation and mRNA expression levels of dentin sialophosphoprotein (DSPP), dentin matrix protein 1 (DMP-1), alkaline phosphatase (ALP), collagen type I (Col-I), osteocalcin (OCN), and runt-related transcription factor 2 (Runx2). The present results demonstrated that the G/PSC scaffold significantly promoted hBMMSC chemotaxis, proliferation and odontogenic differentiation. Therefore, the G/PSC scaffold is a promising material for pulp regeneration.Guibin HuangLiju XuJilin WuSainan WangYanmei DongElsevierarticlepH-neutral bioactive glassComposite scaffoldBone marrow mesenchymal stem cellsMigrationOdontogenic differentiationPulp regenerationPolymers and polymer manufactureTP1080-1185ENPolymer Testing, Vol 93, Iss , Pp 106915- (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	pH-neutral bioactive glass Composite scaffold Bone marrow mesenchymal stem cells Migration Odontogenic differentiation Pulp regeneration Polymers and polymer manufacture TP1080-1185
spellingShingle	pH-neutral bioactive glass Composite scaffold Bone marrow mesenchymal stem cells Migration Odontogenic differentiation Pulp regeneration Polymers and polymer manufacture TP1080-1185 Guibin Huang Liju Xu Jilin Wu Sainan Wang Yanmei Dong Gelatin/bioactive glass composite scaffold for promoting the migration and odontogenic differentiation of bone marrow mesenchymal stem cells
description	Recruiting periapical stem cells into the root canal and inducing their odontogenic differentiation are both vital for pulp regeneration. This study was aimed at developing a kind of bioactive composite scaffolds (G/PSC scaffolds) by combining the pH-neutral bioactive glass named PSC and gelatin. The incorporation of PSC endowed the scaffolds with bioactivity and enhanced its resistance to enzymatic hydrolysis in vitro while maintaining its high water absorption capacity. The G/PSC scaffold with a PSC content of 0.5 mg/mL in 3% gelatin solution showed the best overall performance, released large amounts of silicate ions and maintained a neutral pH after soaking in simulated body fluid (SBF). Human bone marrow mesenchymal stem cells (hBMMSCs) developed good cell morphology with affluent pseudopods on the G/PSC scaffold and infiltrated fairly deep into it. Additionally, hBMMSCs showed increased proliferation and mRNA expression levels of dentin sialophosphoprotein (DSPP), dentin matrix protein 1 (DMP-1), alkaline phosphatase (ALP), collagen type I (Col-I), osteocalcin (OCN), and runt-related transcription factor 2 (Runx2). The present results demonstrated that the G/PSC scaffold significantly promoted hBMMSC chemotaxis, proliferation and odontogenic differentiation. Therefore, the G/PSC scaffold is a promising material for pulp regeneration.
format	article
author	Guibin Huang Liju Xu Jilin Wu Sainan Wang Yanmei Dong
author_facet	Guibin Huang Liju Xu Jilin Wu Sainan Wang Yanmei Dong
author_sort	Guibin Huang
title	Gelatin/bioactive glass composite scaffold for promoting the migration and odontogenic differentiation of bone marrow mesenchymal stem cells
title_short	Gelatin/bioactive glass composite scaffold for promoting the migration and odontogenic differentiation of bone marrow mesenchymal stem cells
title_full	Gelatin/bioactive glass composite scaffold for promoting the migration and odontogenic differentiation of bone marrow mesenchymal stem cells
title_fullStr	Gelatin/bioactive glass composite scaffold for promoting the migration and odontogenic differentiation of bone marrow mesenchymal stem cells
title_full_unstemmed	Gelatin/bioactive glass composite scaffold for promoting the migration and odontogenic differentiation of bone marrow mesenchymal stem cells
title_sort	gelatin/bioactive glass composite scaffold for promoting the migration and odontogenic differentiation of bone marrow mesenchymal stem cells
publisher	Elsevier
publishDate	2021
url	https://doaj.org/article/3e85773806e347d9afee704689bd9df2
work_keys_str_mv	AT guibinhuang gelatinbioactiveglasscompositescaffoldforpromotingthemigrationandodontogenicdifferentiationofbonemarrowmesenchymalstemcells AT lijuxu gelatinbioactiveglasscompositescaffoldforpromotingthemigrationandodontogenicdifferentiationofbonemarrowmesenchymalstemcells AT jilinwu gelatinbioactiveglasscompositescaffoldforpromotingthemigrationandodontogenicdifferentiationofbonemarrowmesenchymalstemcells AT sainanwang gelatinbioactiveglasscompositescaffoldforpromotingthemigrationandodontogenicdifferentiationofbonemarrowmesenchymalstemcells AT yanmeidong gelatinbioactiveglasscompositescaffoldforpromotingthemigrationandodontogenicdifferentiationofbonemarrowmesenchymalstemcells
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Gelatin/bioactive glass composite scaffold for promoting the migration and odontogenic differentiation of bone marrow mesenchymal stem cells

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