Rapid And Sensitive Detection Of Lawsonia Intracellularis In Pigs By Real-Time Loop-Mediated Isothermal Amplification

A simple and rapid real-time loop-mediated isothermal amplification (LAMP) assay designed to detect Lawsonia (L.) intracellularis, an important bacteria causing proliferative enteropathy in pigs. A set of four primers targeting the ubiquinone/menaquinone biosynthesis methylase (ubiE) gene was design...

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Autores principales:	Byung-Yong PARK, Kwan-Seob SHIM, Won-Il KIM, Md Mukter HOSSAIN, Bumseok KIM, Jungkee KWON, Choi-Kyu PARK, Sung-Jin CHO, Inho JO, Ho-Seong CHO
Formato:	article
Lenguaje:	EN
Publicado:	Sciendo 2015
Materias:	lawsonia intracellularis pig porcine proliferative enteropathy real-time loopmediated isothermal amplification Veterinary medicine SF600-1100
Acceso en línea:	https://doaj.org/article/3e86de5e9f9c413c9ac08105b79be3c1
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spelling	oai:doaj.org-article:3e86de5e9f9c413c9ac08105b79be3c12021-11-17T21:27:50ZRapid And Sensitive Detection Of Lawsonia Intracellularis In Pigs By Real-Time Loop-Mediated Isothermal Amplification1820-744810.1515/acve-2015-0002https://doaj.org/article/3e86de5e9f9c413c9ac08105b79be3c12015-03-01T00:00:00Zhttps://doi.org/10.1515/acve-2015-0002https://doaj.org/toc/1820-7448A simple and rapid real-time loop-mediated isothermal amplification (LAMP) assay designed to detect Lawsonia (L.) intracellularis, an important bacteria causing proliferative enteropathy in pigs. A set of four primers targeting the ubiquinone/menaquinone biosynthesis methylase (ubiE) gene was designed for the LAMP reaction. Additionally, serial 10-fold dilutions of cultured L. intracellularis and spiked feces were also used for the optimization of real-time LAMP. The lower limit of the linear range of the assay in L. intracellularis was 1.0 × 100L. intracellularis. Real-time LAMP was 10 and 100 times more sensitive than real-time PCR and conventional PCR detection methods, respectively. Based on testing of 213 porcine fecal samples using real-time LAMP, realtime PCR and PCR, the agreement quotients of real-time LAMP with conventional PCR and with real-time PCR were 0.77 and 0.95, respectively. This study demonstrated that real-time LAMP was a powerful tool for the rapid and sensitive detection of L. intracellularis in porcine fecal samples.Byung-Yong PARKKwan-Seob SHIMWon-Il KIMMd Mukter HOSSAINBumseok KIMJungkee KWONChoi-Kyu PARKSung-Jin CHOInho JOHo-Seong CHOSciendoarticlelawsonia intracellularispigporcine proliferative enteropathyreal-time loopmediated isothermal amplificationVeterinary medicineSF600-1100ENActa Veterinaria, Vol 65, Iss 1, Pp 20-29 (2015)
institution	DOAJ
collection	DOAJ
language	EN
topic	lawsonia intracellularis pig porcine proliferative enteropathy real-time loopmediated isothermal amplification Veterinary medicine SF600-1100
spellingShingle	lawsonia intracellularis pig porcine proliferative enteropathy real-time loopmediated isothermal amplification Veterinary medicine SF600-1100 Byung-Yong PARK Kwan-Seob SHIM Won-Il KIM Md Mukter HOSSAIN Bumseok KIM Jungkee KWON Choi-Kyu PARK Sung-Jin CHO Inho JO Ho-Seong CHO Rapid And Sensitive Detection Of Lawsonia Intracellularis In Pigs By Real-Time Loop-Mediated Isothermal Amplification
description	A simple and rapid real-time loop-mediated isothermal amplification (LAMP) assay designed to detect Lawsonia (L.) intracellularis, an important bacteria causing proliferative enteropathy in pigs. A set of four primers targeting the ubiquinone/menaquinone biosynthesis methylase (ubiE) gene was designed for the LAMP reaction. Additionally, serial 10-fold dilutions of cultured L. intracellularis and spiked feces were also used for the optimization of real-time LAMP. The lower limit of the linear range of the assay in L. intracellularis was 1.0 × 100L. intracellularis. Real-time LAMP was 10 and 100 times more sensitive than real-time PCR and conventional PCR detection methods, respectively. Based on testing of 213 porcine fecal samples using real-time LAMP, realtime PCR and PCR, the agreement quotients of real-time LAMP with conventional PCR and with real-time PCR were 0.77 and 0.95, respectively. This study demonstrated that real-time LAMP was a powerful tool for the rapid and sensitive detection of L. intracellularis in porcine fecal samples.
format	article
author	Byung-Yong PARK Kwan-Seob SHIM Won-Il KIM Md Mukter HOSSAIN Bumseok KIM Jungkee KWON Choi-Kyu PARK Sung-Jin CHO Inho JO Ho-Seong CHO
author_facet	Byung-Yong PARK Kwan-Seob SHIM Won-Il KIM Md Mukter HOSSAIN Bumseok KIM Jungkee KWON Choi-Kyu PARK Sung-Jin CHO Inho JO Ho-Seong CHO
author_sort	Byung-Yong PARK
title	Rapid And Sensitive Detection Of Lawsonia Intracellularis In Pigs By Real-Time Loop-Mediated Isothermal Amplification
title_short	Rapid And Sensitive Detection Of Lawsonia Intracellularis In Pigs By Real-Time Loop-Mediated Isothermal Amplification
title_full	Rapid And Sensitive Detection Of Lawsonia Intracellularis In Pigs By Real-Time Loop-Mediated Isothermal Amplification
title_fullStr	Rapid And Sensitive Detection Of Lawsonia Intracellularis In Pigs By Real-Time Loop-Mediated Isothermal Amplification
title_full_unstemmed	Rapid And Sensitive Detection Of Lawsonia Intracellularis In Pigs By Real-Time Loop-Mediated Isothermal Amplification
title_sort	rapid and sensitive detection of lawsonia intracellularis in pigs by real-time loop-mediated isothermal amplification
publisher	Sciendo
publishDate	2015
url	https://doaj.org/article/3e86de5e9f9c413c9ac08105b79be3c1
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Rapid And Sensitive Detection Of Lawsonia Intracellularis In Pigs By Real-Time Loop-Mediated Isothermal Amplification

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