Variable characteristics of bacteriocin-producing Streptococcus salivarius strains isolated from Malaysian subjects.

<h4>Background</h4>Salivaricins are bacteriocins produced by Streptococcus salivarius, some strains of which can have significant probiotic effects. S. salivarius strains were isolated from Malaysian subjects showing variable antimicrobial activity, metabolic profile, antibiotic suscepti...

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Autores principales:	Abdelahhad Barbour, Koshy Philip
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2014
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/3e88163d23454602839c7b62633f8da8
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spelling	oai:doaj.org-article:3e88163d23454602839c7b62633f8da82021-11-18T08:15:14ZVariable characteristics of bacteriocin-producing Streptococcus salivarius strains isolated from Malaysian subjects.1932-620310.1371/journal.pone.0100541https://doaj.org/article/3e88163d23454602839c7b62633f8da82014-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24941127/?tool=EBIhttps://doaj.org/toc/1932-6203<h4>Background</h4>Salivaricins are bacteriocins produced by Streptococcus salivarius, some strains of which can have significant probiotic effects. S. salivarius strains were isolated from Malaysian subjects showing variable antimicrobial activity, metabolic profile, antibiotic susceptibility and lantibiotic production.<h4>Methodology/principal findings</h4>In this study we report new S. salivarius strains isolated from Malaysian subjects with potential as probiotics. Safety assessment of these strains included their antibiotic susceptibility and metabolic profiles. Genome sequencing using Illumina's MiSeq system was performed for both strains NU10 and YU10 and demonstrating the absence of any known streptococcal virulence determinants indicating that these strains are safe for subsequent use as probiotics. Strain NU10 was found to harbour genes encoding salivaricins A and 9 while strain YU10 was shown to harbour genes encoding salivaricins A3, G32, streptin and slnA1 lantibiotic-like protein. Strain GT2 was shown to harbour genes encoding a large non-lantibiotic bacteriocin (salivaricin-MPS). A new medium for maximum biomass production buffered with 2-(N-morpholino)ethanesulfonic acid (MES) was developed and showed better biomass accumulation compared with other commercial media. Furthermore, we extracted and purified salivaricin 9 (by strain NU10) and salivaricin G32 (by strain YU10) from S. salivarius cells grown aerobically in this medium. In addition to bacteriocin production, S. salivarius strains produced levan-sucrase which was detected by a specific ESI-LC-MS/MS method which indicates additional health benefits from the developed strains.<h4>Conclusion</h4>The current study established the bacteriocin, levan-sucrase production and basic safety features of S. salivarius strains isolated from healthy Malaysian subjects demonstrating their potential for use as probiotics. A new bacteriocin-production medium was developed with potential scale up application for pharmaceuticals and probiotics from S. salivarius generating different lantibiotics. This is relevant for the clinical management of oral cavity and upper respiratory tract in the human population.Abdelahhad BarbourKoshy PhilipPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 9, Iss 6, p e100541 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Abdelahhad Barbour Koshy Philip Variable characteristics of bacteriocin-producing Streptococcus salivarius strains isolated from Malaysian subjects.
description	<h4>Background</h4>Salivaricins are bacteriocins produced by Streptococcus salivarius, some strains of which can have significant probiotic effects. S. salivarius strains were isolated from Malaysian subjects showing variable antimicrobial activity, metabolic profile, antibiotic susceptibility and lantibiotic production.<h4>Methodology/principal findings</h4>In this study we report new S. salivarius strains isolated from Malaysian subjects with potential as probiotics. Safety assessment of these strains included their antibiotic susceptibility and metabolic profiles. Genome sequencing using Illumina's MiSeq system was performed for both strains NU10 and YU10 and demonstrating the absence of any known streptococcal virulence determinants indicating that these strains are safe for subsequent use as probiotics. Strain NU10 was found to harbour genes encoding salivaricins A and 9 while strain YU10 was shown to harbour genes encoding salivaricins A3, G32, streptin and slnA1 lantibiotic-like protein. Strain GT2 was shown to harbour genes encoding a large non-lantibiotic bacteriocin (salivaricin-MPS). A new medium for maximum biomass production buffered with 2-(N-morpholino)ethanesulfonic acid (MES) was developed and showed better biomass accumulation compared with other commercial media. Furthermore, we extracted and purified salivaricin 9 (by strain NU10) and salivaricin G32 (by strain YU10) from S. salivarius cells grown aerobically in this medium. In addition to bacteriocin production, S. salivarius strains produced levan-sucrase which was detected by a specific ESI-LC-MS/MS method which indicates additional health benefits from the developed strains.<h4>Conclusion</h4>The current study established the bacteriocin, levan-sucrase production and basic safety features of S. salivarius strains isolated from healthy Malaysian subjects demonstrating their potential for use as probiotics. A new bacteriocin-production medium was developed with potential scale up application for pharmaceuticals and probiotics from S. salivarius generating different lantibiotics. This is relevant for the clinical management of oral cavity and upper respiratory tract in the human population.
format	article
author	Abdelahhad Barbour Koshy Philip
author_facet	Abdelahhad Barbour Koshy Philip
author_sort	Abdelahhad Barbour
title	Variable characteristics of bacteriocin-producing Streptococcus salivarius strains isolated from Malaysian subjects.
title_short	Variable characteristics of bacteriocin-producing Streptococcus salivarius strains isolated from Malaysian subjects.
title_full	Variable characteristics of bacteriocin-producing Streptococcus salivarius strains isolated from Malaysian subjects.
title_fullStr	Variable characteristics of bacteriocin-producing Streptococcus salivarius strains isolated from Malaysian subjects.
title_full_unstemmed	Variable characteristics of bacteriocin-producing Streptococcus salivarius strains isolated from Malaysian subjects.
title_sort	variable characteristics of bacteriocin-producing streptococcus salivarius strains isolated from malaysian subjects.
publisher	Public Library of Science (PLoS)
publishDate	2014
url	https://doaj.org/article/3e88163d23454602839c7b62633f8da8
work_keys_str_mv	AT abdelahhadbarbour variablecharacteristicsofbacteriocinproducingstreptococcussalivariusstrainsisolatedfrommalaysiansubjects AT koshyphilip variablecharacteristicsofbacteriocinproducingstreptococcussalivariusstrainsisolatedfrommalaysiansubjects
_version_	1718421978626392064

Variable characteristics of bacteriocin-producing Streptococcus salivarius strains isolated from Malaysian subjects.

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