Peptide specific monoclonal antibodies of Leptospiral LigA for acute diagnosis of leptospirosis

Abstract Leptospirosis is underdiagnosed due to low sensitivity, need of specialised equipment, and expensive reagents for serological and molecular diagnosis respectively. Considering the sensitivity, rapidity, inexpensive reagents and collection of clinical samples, the monoclonal antibody based a...

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Autores principales:	Murugesan Kanagavel, Santhanam Shanmughapriya, Kayanam Vijaya Lalitha Aishwarya, Karuppiah Ponmurugan, Kasi Murugan, Naif Abdullah Al-Dhabi, Kalimuthusamy Natarajaseenivasan
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/3e8bec9219ed48b5b73f12e4088af2f5
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spelling	oai:doaj.org-article:3e8bec9219ed48b5b73f12e4088af2f52021-12-02T11:40:12ZPeptide specific monoclonal antibodies of Leptospiral LigA for acute diagnosis of leptospirosis10.1038/s41598-017-03658-02045-2322https://doaj.org/article/3e8bec9219ed48b5b73f12e4088af2f52017-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-03658-0https://doaj.org/toc/2045-2322Abstract Leptospirosis is underdiagnosed due to low sensitivity, need of specialised equipment, and expensive reagents for serological and molecular diagnosis respectively. Considering the sensitivity, rapidity, inexpensive reagents and collection of clinical samples, the monoclonal antibody based antigen detection method from urine samples has been developed and evaluated. LigA (LK90) based B-cell specific epitopes were predicted and synthesised as peptides for the production of monoclonal antibody. LK90543: SNAQKNQGNA (amino acids: 543 to 552), and LK901110: DHHTQSSYTP (amino acids: 1110 to 1119) with VaxiJen score of 1.3719 and 1.2215, respectively were used. Thirty two and 28 urine samples from confirmed and seronegative healthy human subjects, respectively were included for the evaluation of MAb-based dot blot ELISA. The specificity of the evaluated MAbs, P1B1 and P4W2 were found to be in the range of ~93–96%. Moreover, the MAbs did not show cross-reactivity with other bacterial antigens as confirmed by IgG ELISA, further validating its specificity for leptospiral antigens. These findings suggest that the developed MAb based dot blot ELISA is a simple, rapid performed in less than 8 h, inexpensive with a ICER of $8.7/QALY, and affordable in developing countries and area where laboratory facilities are limited.Murugesan KanagavelSanthanam ShanmughapriyaKayanam Vijaya Lalitha AishwaryaKaruppiah PonmuruganKasi MuruganNaif Abdullah Al-DhabiKalimuthusamy NatarajaseenivasanNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-9 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Murugesan Kanagavel Santhanam Shanmughapriya Kayanam Vijaya Lalitha Aishwarya Karuppiah Ponmurugan Kasi Murugan Naif Abdullah Al-Dhabi Kalimuthusamy Natarajaseenivasan Peptide specific monoclonal antibodies of Leptospiral LigA for acute diagnosis of leptospirosis
description	Abstract Leptospirosis is underdiagnosed due to low sensitivity, need of specialised equipment, and expensive reagents for serological and molecular diagnosis respectively. Considering the sensitivity, rapidity, inexpensive reagents and collection of clinical samples, the monoclonal antibody based antigen detection method from urine samples has been developed and evaluated. LigA (LK90) based B-cell specific epitopes were predicted and synthesised as peptides for the production of monoclonal antibody. LK90543: SNAQKNQGNA (amino acids: 543 to 552), and LK901110: DHHTQSSYTP (amino acids: 1110 to 1119) with VaxiJen score of 1.3719 and 1.2215, respectively were used. Thirty two and 28 urine samples from confirmed and seronegative healthy human subjects, respectively were included for the evaluation of MAb-based dot blot ELISA. The specificity of the evaluated MAbs, P1B1 and P4W2 were found to be in the range of ~93–96%. Moreover, the MAbs did not show cross-reactivity with other bacterial antigens as confirmed by IgG ELISA, further validating its specificity for leptospiral antigens. These findings suggest that the developed MAb based dot blot ELISA is a simple, rapid performed in less than 8 h, inexpensive with a ICER of $8.7/QALY, and affordable in developing countries and area where laboratory facilities are limited.
format	article
author	Murugesan Kanagavel Santhanam Shanmughapriya Kayanam Vijaya Lalitha Aishwarya Karuppiah Ponmurugan Kasi Murugan Naif Abdullah Al-Dhabi Kalimuthusamy Natarajaseenivasan
author_facet	Murugesan Kanagavel Santhanam Shanmughapriya Kayanam Vijaya Lalitha Aishwarya Karuppiah Ponmurugan Kasi Murugan Naif Abdullah Al-Dhabi Kalimuthusamy Natarajaseenivasan
author_sort	Murugesan Kanagavel
title	Peptide specific monoclonal antibodies of Leptospiral LigA for acute diagnosis of leptospirosis
title_short	Peptide specific monoclonal antibodies of Leptospiral LigA for acute diagnosis of leptospirosis
title_full	Peptide specific monoclonal antibodies of Leptospiral LigA for acute diagnosis of leptospirosis
title_fullStr	Peptide specific monoclonal antibodies of Leptospiral LigA for acute diagnosis of leptospirosis
title_full_unstemmed	Peptide specific monoclonal antibodies of Leptospiral LigA for acute diagnosis of leptospirosis
title_sort	peptide specific monoclonal antibodies of leptospiral liga for acute diagnosis of leptospirosis
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/3e8bec9219ed48b5b73f12e4088af2f5
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Peptide specific monoclonal antibodies of Leptospiral LigA for acute diagnosis of leptospirosis

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