Association Analysis of the HLA-G Upstream Polymorphism Rs1736933 with the Recurrent Spontaneous Abortion

BACKGROUND AND OBJECTIVE: Studies have suggested that HLA-G is involved in protecting the fetus against the mother's immune system, and upstream polymorphisms of this gene may influence pregnancy success by effecting its expression. The aim of this study was to evaluate the association of upstr...

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Autores principales:	Z Najafi, M Khalaj Kondori, MA Hosseinpour Feizi, Sh Abbasaliizadeh
Formato:	article
Lenguaje:	EN FA
Publicado:	Babol University of Medical Sciences 2020
Materias:	recurrent abortion polymorphism hla-g association studies. Medicine R Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/3e8cf2d2a178445990df62983069f44c
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spelling	oai:doaj.org-article:3e8cf2d2a178445990df62983069f44c2021-11-09T10:15:54ZAssociation Analysis of the HLA-G Upstream Polymorphism Rs1736933 with the Recurrent Spontaneous Abortion1561-41072251-7170https://doaj.org/article/3e8cf2d2a178445990df62983069f44c2020-03-01T00:00:00Zhttp://jbums.org/article-1-9051-en.htmlhttps://doaj.org/toc/1561-4107https://doaj.org/toc/2251-7170BACKGROUND AND OBJECTIVE: Studies have suggested that HLA-G is involved in protecting the fetus against the mother's immune system, and upstream polymorphisms of this gene may influence pregnancy success by effecting its expression. The aim of this study was to evaluate the association of upstream HLA-G gene polymorphism, rs1736933, with recurrent spontaneous abortion in East Azerbaijan women population. METHODS: This case-control study was performed on 80 women with at least one child and without any history of abortion, and 100 women with at least two recurrent spontaneous abortions which were diagnosed by gynecologist. 2 ml of blood was obtained from the participants and after DNA extraction, a segment of the HLA-G gene promoter was amplified by PCR and analyzed by agarose gel electrophoresis. PCR products were sequenced and genotyped. Allelic and genotypic frequencies of patient and control groups were compared. FINDINGS: Frequency of AA, CA, CC genotypes in the patient group were 8(8%), 37(37%), 55(55%) and in the control group were 16(20%), 33(41.25%), 31(38.75%) respectively. Comparison of genotypic frequencies between the two groups showed that CC homozygous genotype was associated with recurrent abortion (p= 0.015). Statistical analysis also showed a significant difference between the allelic frequencies of the two groups (p= 0.019). CONCLUSION: The results of this study showed that the upstream HLA-G gene polymorphism, rs1736933, is associated with the recurrent abortion in East Azerbaijan women population.Z NajafiM Khalaj KondoriMA Hosseinpour FeiziSh AbbasaliizadehBabol University of Medical Sciencesarticlerecurrent abortionpolymorphismhla-gassociation studies.MedicineRMedicine (General)R5-920ENFAMajallah-i Dānishgāh-i ̒Ulūm-i Pizishkī-i Bābul, Vol 22, Iss 1, Pp 175-180 (2020)
institution	DOAJ
collection	DOAJ
language	EN FA
topic	recurrent abortion polymorphism hla-g association studies. Medicine R Medicine (General) R5-920
spellingShingle	recurrent abortion polymorphism hla-g association studies. Medicine R Medicine (General) R5-920 Z Najafi M Khalaj Kondori MA Hosseinpour Feizi Sh Abbasaliizadeh Association Analysis of the HLA-G Upstream Polymorphism Rs1736933 with the Recurrent Spontaneous Abortion
description	BACKGROUND AND OBJECTIVE: Studies have suggested that HLA-G is involved in protecting the fetus against the mother's immune system, and upstream polymorphisms of this gene may influence pregnancy success by effecting its expression. The aim of this study was to evaluate the association of upstream HLA-G gene polymorphism, rs1736933, with recurrent spontaneous abortion in East Azerbaijan women population. METHODS: This case-control study was performed on 80 women with at least one child and without any history of abortion, and 100 women with at least two recurrent spontaneous abortions which were diagnosed by gynecologist. 2 ml of blood was obtained from the participants and after DNA extraction, a segment of the HLA-G gene promoter was amplified by PCR and analyzed by agarose gel electrophoresis. PCR products were sequenced and genotyped. Allelic and genotypic frequencies of patient and control groups were compared. FINDINGS: Frequency of AA, CA, CC genotypes in the patient group were 8(8%), 37(37%), 55(55%) and in the control group were 16(20%), 33(41.25%), 31(38.75%) respectively. Comparison of genotypic frequencies between the two groups showed that CC homozygous genotype was associated with recurrent abortion (p= 0.015). Statistical analysis also showed a significant difference between the allelic frequencies of the two groups (p= 0.019). CONCLUSION: The results of this study showed that the upstream HLA-G gene polymorphism, rs1736933, is associated with the recurrent abortion in East Azerbaijan women population.
format	article
author	Z Najafi M Khalaj Kondori MA Hosseinpour Feizi Sh Abbasaliizadeh
author_facet	Z Najafi M Khalaj Kondori MA Hosseinpour Feizi Sh Abbasaliizadeh
author_sort	Z Najafi
title	Association Analysis of the HLA-G Upstream Polymorphism Rs1736933 with the Recurrent Spontaneous Abortion
title_short	Association Analysis of the HLA-G Upstream Polymorphism Rs1736933 with the Recurrent Spontaneous Abortion
title_full	Association Analysis of the HLA-G Upstream Polymorphism Rs1736933 with the Recurrent Spontaneous Abortion
title_fullStr	Association Analysis of the HLA-G Upstream Polymorphism Rs1736933 with the Recurrent Spontaneous Abortion
title_full_unstemmed	Association Analysis of the HLA-G Upstream Polymorphism Rs1736933 with the Recurrent Spontaneous Abortion
title_sort	association analysis of the hla-g upstream polymorphism rs1736933 with the recurrent spontaneous abortion
publisher	Babol University of Medical Sciences
publishDate	2020
url	https://doaj.org/article/3e8cf2d2a178445990df62983069f44c
work_keys_str_mv	AT znajafi associationanalysisofthehlagupstreampolymorphismrs1736933withtherecurrentspontaneousabortion AT mkhalajkondori associationanalysisofthehlagupstreampolymorphismrs1736933withtherecurrentspontaneousabortion AT mahosseinpourfeizi associationanalysisofthehlagupstreampolymorphismrs1736933withtherecurrentspontaneousabortion AT shabbasaliizadeh associationanalysisofthehlagupstreampolymorphismrs1736933withtherecurrentspontaneousabortion
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Association Analysis of the HLA-G Upstream Polymorphism Rs1736933 with the Recurrent Spontaneous Abortion

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