Fabrication and characterization of 3D-printed gellan gum/starch composite scaffold for Schwann cells growth

Peripheral nerve injury has seriously affected patient’s health and life. Schwann cells play an important role in peripheral nerve regeneration. However, the effect of the current tissue engineered scaffolds for promoting Schwann cells growth is still not as good as that of autologous graft. In this...

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Autores principales:	Zhang Liling, Zheng Tiantian, Wu Linliang, Han Qi, Chen Shiyu, Kong Yan, Li Guicai, Ma Lei, Wu Hong, Zhao Yahong, Yu Yinxian, Yang Yumin
Formato:	article
Lenguaje:	EN
Publicado:	De Gruyter 2021
Materias:	3d printing gellan gum starch peripheral nerve scaffolds Technology T Chemical technology TP1-1185 Physical and theoretical chemistry QD450-801
Acceso en línea:	https://doaj.org/article/3e99d3f7ff0441b2ae3e880e544559a4
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spelling	oai:doaj.org-article:3e99d3f7ff0441b2ae3e880e544559a42021-12-05T14:10:57ZFabrication and characterization of 3D-printed gellan gum/starch composite scaffold for Schwann cells growth2191-909710.1515/ntrev-2021-0004https://doaj.org/article/3e99d3f7ff0441b2ae3e880e544559a42021-03-01T00:00:00Zhttps://doi.org/10.1515/ntrev-2021-0004https://doaj.org/toc/2191-9097Peripheral nerve injury has seriously affected patient’s health and life. Schwann cells play an important role in peripheral nerve regeneration. However, the effect of the current tissue engineered scaffolds for promoting Schwann cells growth is still not as good as that of autologous graft. In this study, new developed three-dimensional gellan gum/starch (GG/ST) scaffolds with various printing gap for Schwann cells growth were prepared by 3D printing technology. Various physiochemical characterizations of the printed scaffolds were performed including morphology, rheological behavior, swelling ratio, and degradation behavior. The cytotoxicity and biocompatibility of the scaffolds were evaluated using L929 fibroblasts and RSC96 Schwann cells, respectively. The results displayed that the GG/ST scaffold exhibited a porous network structure. The cross-sectional pore density of the hydrogel had a tendency to increase with the ascending printing gap. The swelling rate and degradation rate of the hydrogel gradually increased and eventually reached an equilibrium state. The rheological test results showed that the scaffolds had good printability. MTT cytotoxicity test and CCK-8 cell proliferation test displayed that the scaffold was nontoxic, and Schwann cells could grow well on the scaffold after 5 days of culture, whereas the number of cells on the scaffold with the printing gap of 3 mm was the largest. These results indicated that the GG/ST scaffold prepared by 3D printing technology may have a potential application in peripheral nerve regeneration.Zhang LilingZheng TiantianWu LinliangHan QiChen ShiyuKong YanLi GuicaiMa LeiWu HongZhao YahongYu YinxianYang YuminDe Gruyterarticle3d printinggellan gumstarchperipheral nervescaffoldsTechnologyTChemical technologyTP1-1185Physical and theoretical chemistryQD450-801ENNanotechnology Reviews, Vol 10, Iss 1, Pp 50-61 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	3d printing gellan gum starch peripheral nerve scaffolds Technology T Chemical technology TP1-1185 Physical and theoretical chemistry QD450-801
spellingShingle	3d printing gellan gum starch peripheral nerve scaffolds Technology T Chemical technology TP1-1185 Physical and theoretical chemistry QD450-801 Zhang Liling Zheng Tiantian Wu Linliang Han Qi Chen Shiyu Kong Yan Li Guicai Ma Lei Wu Hong Zhao Yahong Yu Yinxian Yang Yumin Fabrication and characterization of 3D-printed gellan gum/starch composite scaffold for Schwann cells growth
description	Peripheral nerve injury has seriously affected patient’s health and life. Schwann cells play an important role in peripheral nerve regeneration. However, the effect of the current tissue engineered scaffolds for promoting Schwann cells growth is still not as good as that of autologous graft. In this study, new developed three-dimensional gellan gum/starch (GG/ST) scaffolds with various printing gap for Schwann cells growth were prepared by 3D printing technology. Various physiochemical characterizations of the printed scaffolds were performed including morphology, rheological behavior, swelling ratio, and degradation behavior. The cytotoxicity and biocompatibility of the scaffolds were evaluated using L929 fibroblasts and RSC96 Schwann cells, respectively. The results displayed that the GG/ST scaffold exhibited a porous network structure. The cross-sectional pore density of the hydrogel had a tendency to increase with the ascending printing gap. The swelling rate and degradation rate of the hydrogel gradually increased and eventually reached an equilibrium state. The rheological test results showed that the scaffolds had good printability. MTT cytotoxicity test and CCK-8 cell proliferation test displayed that the scaffold was nontoxic, and Schwann cells could grow well on the scaffold after 5 days of culture, whereas the number of cells on the scaffold with the printing gap of 3 mm was the largest. These results indicated that the GG/ST scaffold prepared by 3D printing technology may have a potential application in peripheral nerve regeneration.
format	article
author	Zhang Liling Zheng Tiantian Wu Linliang Han Qi Chen Shiyu Kong Yan Li Guicai Ma Lei Wu Hong Zhao Yahong Yu Yinxian Yang Yumin
author_facet	Zhang Liling Zheng Tiantian Wu Linliang Han Qi Chen Shiyu Kong Yan Li Guicai Ma Lei Wu Hong Zhao Yahong Yu Yinxian Yang Yumin
author_sort	Zhang Liling
title	Fabrication and characterization of 3D-printed gellan gum/starch composite scaffold for Schwann cells growth
title_short	Fabrication and characterization of 3D-printed gellan gum/starch composite scaffold for Schwann cells growth
title_full	Fabrication and characterization of 3D-printed gellan gum/starch composite scaffold for Schwann cells growth
title_fullStr	Fabrication and characterization of 3D-printed gellan gum/starch composite scaffold for Schwann cells growth
title_full_unstemmed	Fabrication and characterization of 3D-printed gellan gum/starch composite scaffold for Schwann cells growth
title_sort	fabrication and characterization of 3d-printed gellan gum/starch composite scaffold for schwann cells growth
publisher	De Gruyter
publishDate	2021
url	https://doaj.org/article/3e99d3f7ff0441b2ae3e880e544559a4
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Fabrication and characterization of 3D-printed gellan gum/starch composite scaffold for Schwann cells growth

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