Isolation, identification, and biological characteristics of Clostridium sartagoforme from rabbit.

In order to develop microbial additives for rabbit feed, a spore-forming bacteria was isolated from the feces of Hyla rabbit using reinforced clostridium medium (RCM). The 16S rDNA sequence of the bacterium was subjected to pairwise sequence alignment using BLAST; the colony morphology, and physiolo...

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Autores principales:	Ruiguang Gong, Xiangyang Ye, Shuhui Wang, Zhanjun Ren
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/3ea13c8437be40edb92b657c478bd860
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spelling	oai:doaj.org-article:3ea13c8437be40edb92b657c478bd8602021-12-02T20:13:07ZIsolation, identification, and biological characteristics of Clostridium sartagoforme from rabbit.1932-620310.1371/journal.pone.0259715https://doaj.org/article/3ea13c8437be40edb92b657c478bd8602021-01-01T00:00:00Zhttps://doi.org/10.1371/journal.pone.0259715https://doaj.org/toc/1932-6203In order to develop microbial additives for rabbit feed, a spore-forming bacteria was isolated from the feces of Hyla rabbit using reinforced clostridium medium (RCM). The 16S rDNA sequence of the bacterium was subjected to pairwise sequence alignment using BLAST; the colony morphology, and physiological, biochemical, and stress resistance were studied. The results showed that the bacterium was Clostridium sartagoforme, a gram positive anaerobe, which can produce spores. The colony diameter was 0.5 mm-2.5 mm, the diameter of the bacteria was 0.5 μm-1.0 μm × 2.0 μm-6.3 μm, and the spore diameter was 1 μm-1.2 μm × 1 μm-1.2 μm. C. sartagoforme can utilize various sugars and alcohols such as fructose, galactose, sorbitol, and inositol. It secreted cellulase into the extracellular environment to form a transparent hydrolysis circle in Congo red medium, it could not liquify gelatin, and the lysine decarboxylase reaction was positive. In liquid medium it entered the stable growth period after 9 h of inoculation. Additionally, it had good stress resistance with a survival rate that exceeded 53% after gastric juice (pH 2.5) treatment for 3 h, it grew in a medium with a bile salt concentration of 0.3%, and the survival rate exceeded 85% after 10 minutes at 80°C. Moreover, animal testing indicated that this strain has no adverse effects on the morbidity and mortality of rabbits. In summary, C. sartagoforme XN-T4 was isolated from rabbit feces. This bacterium has good resistance to stress, can decompose a variety of monosaccharides and polysaccharides including cellulose, which is relatively harmless for animal health.Ruiguang GongXiangyang YeShuhui WangZhanjun RenPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 16, Iss 11, p e0259715 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Ruiguang Gong Xiangyang Ye Shuhui Wang Zhanjun Ren Isolation, identification, and biological characteristics of Clostridium sartagoforme from rabbit.
description	In order to develop microbial additives for rabbit feed, a spore-forming bacteria was isolated from the feces of Hyla rabbit using reinforced clostridium medium (RCM). The 16S rDNA sequence of the bacterium was subjected to pairwise sequence alignment using BLAST; the colony morphology, and physiological, biochemical, and stress resistance were studied. The results showed that the bacterium was Clostridium sartagoforme, a gram positive anaerobe, which can produce spores. The colony diameter was 0.5 mm-2.5 mm, the diameter of the bacteria was 0.5 μm-1.0 μm × 2.0 μm-6.3 μm, and the spore diameter was 1 μm-1.2 μm × 1 μm-1.2 μm. C. sartagoforme can utilize various sugars and alcohols such as fructose, galactose, sorbitol, and inositol. It secreted cellulase into the extracellular environment to form a transparent hydrolysis circle in Congo red medium, it could not liquify gelatin, and the lysine decarboxylase reaction was positive. In liquid medium it entered the stable growth period after 9 h of inoculation. Additionally, it had good stress resistance with a survival rate that exceeded 53% after gastric juice (pH 2.5) treatment for 3 h, it grew in a medium with a bile salt concentration of 0.3%, and the survival rate exceeded 85% after 10 minutes at 80°C. Moreover, animal testing indicated that this strain has no adverse effects on the morbidity and mortality of rabbits. In summary, C. sartagoforme XN-T4 was isolated from rabbit feces. This bacterium has good resistance to stress, can decompose a variety of monosaccharides and polysaccharides including cellulose, which is relatively harmless for animal health.
format	article
author	Ruiguang Gong Xiangyang Ye Shuhui Wang Zhanjun Ren
author_facet	Ruiguang Gong Xiangyang Ye Shuhui Wang Zhanjun Ren
author_sort	Ruiguang Gong
title	Isolation, identification, and biological characteristics of Clostridium sartagoforme from rabbit.
title_short	Isolation, identification, and biological characteristics of Clostridium sartagoforme from rabbit.
title_full	Isolation, identification, and biological characteristics of Clostridium sartagoforme from rabbit.
title_fullStr	Isolation, identification, and biological characteristics of Clostridium sartagoforme from rabbit.
title_full_unstemmed	Isolation, identification, and biological characteristics of Clostridium sartagoforme from rabbit.
title_sort	isolation, identification, and biological characteristics of clostridium sartagoforme from rabbit.
publisher	Public Library of Science (PLoS)
publishDate	2021
url	https://doaj.org/article/3ea13c8437be40edb92b657c478bd860
work_keys_str_mv	AT ruiguanggong isolationidentificationandbiologicalcharacteristicsofclostridiumsartagoformefromrabbit AT xiangyangye isolationidentificationandbiologicalcharacteristicsofclostridiumsartagoformefromrabbit AT shuhuiwang isolationidentificationandbiologicalcharacteristicsofclostridiumsartagoformefromrabbit AT zhanjunren isolationidentificationandbiologicalcharacteristicsofclostridiumsartagoformefromrabbit
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Isolation, identification, and biological characteristics of Clostridium sartagoforme from rabbit.

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