Phenotypic and Molecular Characterization of Commensal, Community-Acquired and Nosocomial <i>Klebsiella</i> spp.
<i>Klebsiella</i> spp. is a relevant pathogen that can present acquired resistance to almost all available antibiotics, thus representing a serious threat for public health. While most studies have been focused on isolates causing community-acquired and nosocomial infections, little is k...
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| Autores principales: | , , , , |
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| Formato: | article |
| Lenguaje: | EN |
| Publicado: |
MDPI AG
2021
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| Materias: | |
| Acceso en línea: | https://doaj.org/article/3eb7dbd4d08d4ce3a217fbc73ac77e6a |
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| Sumario: | <i>Klebsiella</i> spp. is a relevant pathogen that can present acquired resistance to almost all available antibiotics, thus representing a serious threat for public health. While most studies have been focused on isolates causing community-acquired and nosocomial infections, little is known about the commensal isolates colonizing healthy subjects. We describe the molecular identification and the phenotypic characterization of commensal <i>Klebsiella</i> spp. from breast milk of healthy women and faeces from healthy breast-fed infants, which were compared with isolates from community-acquired infections and from a nosocomial NICU outbreak. The phylogenetic analysis of a 454-bp sequence of the <i>rpoB</i> gene was useful for species identification (<i>K. pneumoniae</i>, <i>K. variicola</i>, <i>K. quasipneumoniae</i>, <i>K. oxytoca</i>, <i>K. grimontii</i>, <i>K. michiganensis</i>, <i>Raoultella planticola</i> and <i>R. ornithinolytica</i>), previously misidentified as <i>K. pneumoniae</i> or <i>K. oxytoca</i> by biochemical methods. Globally, we report that commensal strains present virulence traits (virulence genes, siderophores and biofilms) comparable to community-acquired and NICU-infective isolates, thus suggesting that the human microbiota could constitute a reservoir for infection. Isolates causing NICU outbreak were multi-drug resistant (MDR) and ESBLs producers, although an imipenem-resistant commensal MDR <i>K. quasipneumoniae</i> isolate was also found. A commensal <i>K. pneumoniae</i> strain showed a potent bacteriocin-like inhibitory activity against MDR <i>Klebsiella</i> isolates, thus highlighting the potential role of commensal <i>Klebsiella</i> spp. in health and disease. |
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