Phenotypic and Molecular Characterization of Commensal, Community-Acquired and Nosocomial <i>Klebsiella</i> spp.

<i>Klebsiella</i> spp. is a relevant pathogen that can present acquired resistance to almost all available antibiotics, thus representing a serious threat for public health. While most studies have been focused on isolates causing community-acquired and nosocomial infections, little is k...

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Autores principales:	Marta Gómez, Arancha Valverde, Rosa del Campo, Juan Miguel Rodríguez, Antonio Maldonado-Barragán
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	<i>Klebsiella</i> <i>rpoB</i> virulence siderophores biofilms antibiotic resistance Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/3eb7dbd4d08d4ce3a217fbc73ac77e6a
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spelling	oai:doaj.org-article:3eb7dbd4d08d4ce3a217fbc73ac77e6a2021-11-25T18:25:18ZPhenotypic and Molecular Characterization of Commensal, Community-Acquired and Nosocomial <i>Klebsiella</i> spp.10.3390/microorganisms91123442076-2607https://doaj.org/article/3eb7dbd4d08d4ce3a217fbc73ac77e6a2021-11-01T00:00:00Zhttps://www.mdpi.com/2076-2607/9/11/2344https://doaj.org/toc/2076-2607<i>Klebsiella</i> spp. is a relevant pathogen that can present acquired resistance to almost all available antibiotics, thus representing a serious threat for public health. While most studies have been focused on isolates causing community-acquired and nosocomial infections, little is known about the commensal isolates colonizing healthy subjects. We describe the molecular identification and the phenotypic characterization of commensal <i>Klebsiella</i> spp. from breast milk of healthy women and faeces from healthy breast-fed infants, which were compared with isolates from community-acquired infections and from a nosocomial NICU outbreak. The phylogenetic analysis of a 454-bp sequence of the <i>rpoB</i> gene was useful for species identification (<i>K. pneumoniae</i>, <i>K. variicola</i>, <i>K. quasipneumoniae</i>, <i>K. oxytoca</i>, <i>K. grimontii</i>, <i>K. michiganensis</i>, <i>Raoultella planticola</i> and <i>R. ornithinolytica</i>), previously misidentified as <i>K. pneumoniae</i> or <i>K. oxytoca</i> by biochemical methods. Globally, we report that commensal strains present virulence traits (virulence genes, siderophores and biofilms) comparable to community-acquired and NICU-infective isolates, thus suggesting that the human microbiota could constitute a reservoir for infection. Isolates causing NICU outbreak were multi-drug resistant (MDR) and ESBLs producers, although an imipenem-resistant commensal MDR <i>K. quasipneumoniae</i> isolate was also found. A commensal <i>K. pneumoniae</i> strain showed a potent bacteriocin-like inhibitory activity against MDR <i>Klebsiella</i> isolates, thus highlighting the potential role of commensal <i>Klebsiella</i> spp. in health and disease.Marta GómezArancha ValverdeRosa del CampoJuan Miguel RodríguezAntonio Maldonado-BarragánMDPI AGarticle<i>Klebsiella</i><i>rpoB</i>virulencesiderophoresbiofilmsantibiotic resistanceBiology (General)QH301-705.5ENMicroorganisms, Vol 9, Iss 2344, p 2344 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	<i>Klebsiella</i> <i>rpoB</i> virulence siderophores biofilms antibiotic resistance Biology (General) QH301-705.5
spellingShingle	<i>Klebsiella</i> <i>rpoB</i> virulence siderophores biofilms antibiotic resistance Biology (General) QH301-705.5 Marta Gómez Arancha Valverde Rosa del Campo Juan Miguel Rodríguez Antonio Maldonado-Barragán Phenotypic and Molecular Characterization of Commensal, Community-Acquired and Nosocomial <i>Klebsiella</i> spp.
description	<i>Klebsiella</i> spp. is a relevant pathogen that can present acquired resistance to almost all available antibiotics, thus representing a serious threat for public health. While most studies have been focused on isolates causing community-acquired and nosocomial infections, little is known about the commensal isolates colonizing healthy subjects. We describe the molecular identification and the phenotypic characterization of commensal <i>Klebsiella</i> spp. from breast milk of healthy women and faeces from healthy breast-fed infants, which were compared with isolates from community-acquired infections and from a nosocomial NICU outbreak. The phylogenetic analysis of a 454-bp sequence of the <i>rpoB</i> gene was useful for species identification (<i>K. pneumoniae</i>, <i>K. variicola</i>, <i>K. quasipneumoniae</i>, <i>K. oxytoca</i>, <i>K. grimontii</i>, <i>K. michiganensis</i>, <i>Raoultella planticola</i> and <i>R. ornithinolytica</i>), previously misidentified as <i>K. pneumoniae</i> or <i>K. oxytoca</i> by biochemical methods. Globally, we report that commensal strains present virulence traits (virulence genes, siderophores and biofilms) comparable to community-acquired and NICU-infective isolates, thus suggesting that the human microbiota could constitute a reservoir for infection. Isolates causing NICU outbreak were multi-drug resistant (MDR) and ESBLs producers, although an imipenem-resistant commensal MDR <i>K. quasipneumoniae</i> isolate was also found. A commensal <i>K. pneumoniae</i> strain showed a potent bacteriocin-like inhibitory activity against MDR <i>Klebsiella</i> isolates, thus highlighting the potential role of commensal <i>Klebsiella</i> spp. in health and disease.
format	article
author	Marta Gómez Arancha Valverde Rosa del Campo Juan Miguel Rodríguez Antonio Maldonado-Barragán
author_facet	Marta Gómez Arancha Valverde Rosa del Campo Juan Miguel Rodríguez Antonio Maldonado-Barragán
author_sort	Marta Gómez
title	Phenotypic and Molecular Characterization of Commensal, Community-Acquired and Nosocomial <i>Klebsiella</i> spp.
title_short	Phenotypic and Molecular Characterization of Commensal, Community-Acquired and Nosocomial <i>Klebsiella</i> spp.
title_full	Phenotypic and Molecular Characterization of Commensal, Community-Acquired and Nosocomial <i>Klebsiella</i> spp.
title_fullStr	Phenotypic and Molecular Characterization of Commensal, Community-Acquired and Nosocomial <i>Klebsiella</i> spp.
title_full_unstemmed	Phenotypic and Molecular Characterization of Commensal, Community-Acquired and Nosocomial <i>Klebsiella</i> spp.
title_sort	phenotypic and molecular characterization of commensal, community-acquired and nosocomial <i>klebsiella</i> spp.
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/3eb7dbd4d08d4ce3a217fbc73ac77e6a
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Phenotypic and Molecular Characterization of Commensal, Community-Acquired and Nosocomial <i>Klebsiella</i> spp.

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