Detection of haplotype mutations of the MD-2 gene promoter associated with Der p2-induced allergy using a nanostructured biosensor

Yu-Ting Chin,1 En-Chih Liao,2 Chia-Che Wu,1 Gou-Jen Wang,1,3,4 Jaw-Ji Tsai2 1Department of Mechanical Engineering, National Chung-Hsing University, 2Department of Medical Education and Research, Taichung Veterans General Hospital, 3Graduate Institute of Biomedical Engineering, 4PhD Program in Tissu...

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Autores principales:	Chin YT, Liao EC, Wu CC, Wang GJ, Tsai JJ
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2014
Materias:	Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/3ebca8182d0e45609691f1b6976803f0
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spelling	oai:doaj.org-article:3ebca8182d0e45609691f1b6976803f02021-12-02T02:04:24ZDetection of haplotype mutations of the MD-2 gene promoter associated with Der p2-induced allergy using a nanostructured biosensor1178-2013https://doaj.org/article/3ebca8182d0e45609691f1b6976803f02014-03-01T00:00:00Zhttp://www.dovepress.com/detection-of-haplotype-mutations-of-the-md-2-gene-promoter-associated--a16090https://doaj.org/toc/1178-2013 Yu-Ting Chin,1 En-Chih Liao,2 Chia-Che Wu,1 Gou-Jen Wang,1,3,4 Jaw-Ji Tsai2 1Department of Mechanical Engineering, National Chung-Hsing University, 2Department of Medical Education and Research, Taichung Veterans General Hospital, 3Graduate Institute of Biomedical Engineering, 4PhD Program in Tissue Engineering and Regenerative Medicine, National Chung-Hsing University, Taichung, Taiwan Abstract: Group 2 allergens (Der p2) have been reported to be a major cause of the human immune response to dust mite allergens. In this study, we have demonstrated for the first time the effective differentiation between haplotype mutation and normal genes in the MD-2 gene promoter using a nanostructured biosensor. A 70-mer gene fragment containing the haplotype of two single nucleotide polymorphisms in the MD-2 gene promoter region was used as a probe to detect haplotype mutations associated with Der p2-induced allergy. Discrimination was achieved using electrochemical impedance spectroscopy. The discrimination experiments employed 30 haplotype mutation samples and 30 normal target samples. The haplotype mutation samples and normal target samples could be clearly discriminated, even using samples produced by a five-cycle polymerase chain reaction process. The time and cost of sample preparation for the polymerase chain reaction process in the clinical setting can thus be reduced. Keywords: haplotype mutation detection, nanostructured biosensor, MD-2 gene promoter, electrochemical impedance spectroscopyChin YTLiao ECWu CCWang GJTsai JJDove Medical PressarticleMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2014, Iss Issue 1, Pp 1403-1412 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine (General) R5-920
spellingShingle	Medicine (General) R5-920 Chin YT Liao EC Wu CC Wang GJ Tsai JJ Detection of haplotype mutations of the MD-2 gene promoter associated with Der p2-induced allergy using a nanostructured biosensor
description	Yu-Ting Chin,1 En-Chih Liao,2 Chia-Che Wu,1 Gou-Jen Wang,1,3,4 Jaw-Ji Tsai2 1Department of Mechanical Engineering, National Chung-Hsing University, 2Department of Medical Education and Research, Taichung Veterans General Hospital, 3Graduate Institute of Biomedical Engineering, 4PhD Program in Tissue Engineering and Regenerative Medicine, National Chung-Hsing University, Taichung, Taiwan Abstract: Group 2 allergens (Der p2) have been reported to be a major cause of the human immune response to dust mite allergens. In this study, we have demonstrated for the first time the effective differentiation between haplotype mutation and normal genes in the MD-2 gene promoter using a nanostructured biosensor. A 70-mer gene fragment containing the haplotype of two single nucleotide polymorphisms in the MD-2 gene promoter region was used as a probe to detect haplotype mutations associated with Der p2-induced allergy. Discrimination was achieved using electrochemical impedance spectroscopy. The discrimination experiments employed 30 haplotype mutation samples and 30 normal target samples. The haplotype mutation samples and normal target samples could be clearly discriminated, even using samples produced by a five-cycle polymerase chain reaction process. The time and cost of sample preparation for the polymerase chain reaction process in the clinical setting can thus be reduced. Keywords: haplotype mutation detection, nanostructured biosensor, MD-2 gene promoter, electrochemical impedance spectroscopy
format	article
author	Chin YT Liao EC Wu CC Wang GJ Tsai JJ
author_facet	Chin YT Liao EC Wu CC Wang GJ Tsai JJ
author_sort	Chin YT
title	Detection of haplotype mutations of the MD-2 gene promoter associated with Der p2-induced allergy using a nanostructured biosensor
title_short	Detection of haplotype mutations of the MD-2 gene promoter associated with Der p2-induced allergy using a nanostructured biosensor
title_full	Detection of haplotype mutations of the MD-2 gene promoter associated with Der p2-induced allergy using a nanostructured biosensor
title_fullStr	Detection of haplotype mutations of the MD-2 gene promoter associated with Der p2-induced allergy using a nanostructured biosensor
title_full_unstemmed	Detection of haplotype mutations of the MD-2 gene promoter associated with Der p2-induced allergy using a nanostructured biosensor
title_sort	detection of haplotype mutations of the md-2 gene promoter associated with der p2-induced allergy using a nanostructured biosensor
publisher	Dove Medical Press
publishDate	2014
url	https://doaj.org/article/3ebca8182d0e45609691f1b6976803f0
work_keys_str_mv	AT chinyt detectionofhaplotypemutationsofthemd2genepromoterassociatedwithderp2inducedallergyusingananostructuredbiosensor AT liaoec detectionofhaplotypemutationsofthemd2genepromoterassociatedwithderp2inducedallergyusingananostructuredbiosensor AT wucc detectionofhaplotypemutationsofthemd2genepromoterassociatedwithderp2inducedallergyusingananostructuredbiosensor AT wanggj detectionofhaplotypemutationsofthemd2genepromoterassociatedwithderp2inducedallergyusingananostructuredbiosensor AT tsaijj detectionofhaplotypemutationsofthemd2genepromoterassociatedwithderp2inducedallergyusingananostructuredbiosensor
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Detection of haplotype mutations of the MD-2 gene promoter associated with Der p2-induced allergy using a nanostructured biosensor

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