Identification of Potential Host Plants of Sap-Sucking Insects (Hemiptera: Cicadellidae) Using Anchored Hybrid By-Catch Data

Reliable host plant records are available for only a small fraction of herbivorous insect species, despite their potential agricultural importance. Most available data on insect–plant associations have been obtained through field observations of occurrences of insects on particular plants. Molecular...

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Autores principales:	Yanghui Cao, Christopher H. Dietrich
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	leafhoppers host plants insect–plant association anchored hybrid enrichment Science Q
Acceso en línea:	https://doaj.org/article/3ed04db8f8c746e0b81e1117ef0e75e8
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spelling	oai:doaj.org-article:3ed04db8f8c746e0b81e1117ef0e75e82021-11-25T17:59:07ZIdentification of Potential Host Plants of Sap-Sucking Insects (Hemiptera: Cicadellidae) Using Anchored Hybrid By-Catch Data10.3390/insects121109642075-4450https://doaj.org/article/3ed04db8f8c746e0b81e1117ef0e75e82021-10-01T00:00:00Zhttps://www.mdpi.com/2075-4450/12/11/964https://doaj.org/toc/2075-4450Reliable host plant records are available for only a small fraction of herbivorous insect species, despite their potential agricultural importance. Most available data on insect–plant associations have been obtained through field observations of occurrences of insects on particular plants. Molecular methods have more recently been used to identify potential host plants using DNA extracted from insects, but most prior studies using these methods have focused on chewing insects that ingest tissues expected to contain large quantities of plant DNA. Screening of Illumina data obtained from sap feeders of the hemipteran family Cicadellidae (leafhoppers) using anchored hybrid enrichment indicates that, despite feeding on plant fluids, these insects often contain detectable quantities of plant DNA. Although inclusion of probes for bacterial <i>16S</i> in the original anchored hybrid probe kit yielded relatively high detection rates for chloroplast <i>16S</i>, the Illumina short reads also, in some cases, included DNA for various plant barcode genes as “by-catch”. Detection rates were generally only slightly higher for Typhlocybinae, which feed preferentially on parenchyma cell contents, compared to other groups of leafhoppers that feed preferentially on phloem or xylem. These results indicate that next-generation sequencing provides a powerful tool to investigate the specific association between individual insect and plant species.Yanghui CaoChristopher H. DietrichMDPI AGarticleleafhoppershost plantsinsect–plant associationanchored hybrid enrichmentScienceQENInsects, Vol 12, Iss 964, p 964 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	leafhoppers host plants insect–plant association anchored hybrid enrichment Science Q
spellingShingle	leafhoppers host plants insect–plant association anchored hybrid enrichment Science Q Yanghui Cao Christopher H. Dietrich Identification of Potential Host Plants of Sap-Sucking Insects (Hemiptera: Cicadellidae) Using Anchored Hybrid By-Catch Data
description	Reliable host plant records are available for only a small fraction of herbivorous insect species, despite their potential agricultural importance. Most available data on insect–plant associations have been obtained through field observations of occurrences of insects on particular plants. Molecular methods have more recently been used to identify potential host plants using DNA extracted from insects, but most prior studies using these methods have focused on chewing insects that ingest tissues expected to contain large quantities of plant DNA. Screening of Illumina data obtained from sap feeders of the hemipteran family Cicadellidae (leafhoppers) using anchored hybrid enrichment indicates that, despite feeding on plant fluids, these insects often contain detectable quantities of plant DNA. Although inclusion of probes for bacterial <i>16S</i> in the original anchored hybrid probe kit yielded relatively high detection rates for chloroplast <i>16S</i>, the Illumina short reads also, in some cases, included DNA for various plant barcode genes as “by-catch”. Detection rates were generally only slightly higher for Typhlocybinae, which feed preferentially on parenchyma cell contents, compared to other groups of leafhoppers that feed preferentially on phloem or xylem. These results indicate that next-generation sequencing provides a powerful tool to investigate the specific association between individual insect and plant species.
format	article
author	Yanghui Cao Christopher H. Dietrich
author_facet	Yanghui Cao Christopher H. Dietrich
author_sort	Yanghui Cao
title	Identification of Potential Host Plants of Sap-Sucking Insects (Hemiptera: Cicadellidae) Using Anchored Hybrid By-Catch Data
title_short	Identification of Potential Host Plants of Sap-Sucking Insects (Hemiptera: Cicadellidae) Using Anchored Hybrid By-Catch Data
title_full	Identification of Potential Host Plants of Sap-Sucking Insects (Hemiptera: Cicadellidae) Using Anchored Hybrid By-Catch Data
title_fullStr	Identification of Potential Host Plants of Sap-Sucking Insects (Hemiptera: Cicadellidae) Using Anchored Hybrid By-Catch Data
title_full_unstemmed	Identification of Potential Host Plants of Sap-Sucking Insects (Hemiptera: Cicadellidae) Using Anchored Hybrid By-Catch Data
title_sort	identification of potential host plants of sap-sucking insects (hemiptera: cicadellidae) using anchored hybrid by-catch data
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/3ed04db8f8c746e0b81e1117ef0e75e8
work_keys_str_mv	AT yanghuicao identificationofpotentialhostplantsofsapsuckinginsectshemipteracicadellidaeusinganchoredhybridbycatchdata AT christopherhdietrich identificationofpotentialhostplantsofsapsuckinginsectshemipteracicadellidaeusinganchoredhybridbycatchdata
_version_	1718411737111199744

Identification of Potential Host Plants of Sap-Sucking Insects (Hemiptera: Cicadellidae) Using Anchored Hybrid By-Catch Data

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