Single-cell RNA sequencing reveals developmental heterogeneity among Plasmodium berghei sporozoites

Abstract In the malaria-causing parasite’s life cycle, Plasmodium sporozoites must travel from the midgut of a mosquito to the salivary glands before they can infect a mammalian host. However, only a fraction of sporozoites complete the journey. Since salivary gland invasion is required for transmis...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Anthony A. Ruberto, Caitlin Bourke, Nicolas Merienne, Thomas Obadia, Rogerio Amino, Ivo Mueller
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/3ed21ec8665144f28e647dfbfbc05286
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:3ed21ec8665144f28e647dfbfbc05286
record_format	dspace
spelling	oai:doaj.org-article:3ed21ec8665144f28e647dfbfbc052862021-12-02T14:28:22ZSingle-cell RNA sequencing reveals developmental heterogeneity among Plasmodium berghei sporozoites10.1038/s41598-021-82914-w2045-2322https://doaj.org/article/3ed21ec8665144f28e647dfbfbc052862021-02-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-82914-whttps://doaj.org/toc/2045-2322Abstract In the malaria-causing parasite’s life cycle, Plasmodium sporozoites must travel from the midgut of a mosquito to the salivary glands before they can infect a mammalian host. However, only a fraction of sporozoites complete the journey. Since salivary gland invasion is required for transmission of sporozoites, insights at the molecular level can contribute to strategies for malaria prevention. Recent advances in single-cell RNA sequencing provide an opportunity to assess sporozoite heterogeneity at a resolution unattainable by bulk RNA sequencing methods. In this study, we use a droplet-based single-cell RNA sequencing workflow to analyze the transcriptomes of over 8000 Plasmodium berghei sporozoites derived from the midguts and salivary glands of Anopheles stephensi mosquitoes. The detection of known marker genes confirms the successful capture and sequencing of samples composed of a mixed population of sporozoites. Using data integration, clustering, and trajectory analyses, we reveal differences in gene expression profiles of individual sporozoites, and identify both annotated and unannotated markers associated with sporozoite development. Our work highlights the utility of a high-throughput workflow for the transcriptomic profiling of Plasmodium sporozoites, and provides new insights into gene usage during the parasite’s development in the mosquito.Anthony A. RubertoCaitlin BourkeNicolas MerienneThomas ObadiaRogerio AminoIvo MuellerNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-14 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Anthony A. Ruberto Caitlin Bourke Nicolas Merienne Thomas Obadia Rogerio Amino Ivo Mueller Single-cell RNA sequencing reveals developmental heterogeneity among Plasmodium berghei sporozoites
description	Abstract In the malaria-causing parasite’s life cycle, Plasmodium sporozoites must travel from the midgut of a mosquito to the salivary glands before they can infect a mammalian host. However, only a fraction of sporozoites complete the journey. Since salivary gland invasion is required for transmission of sporozoites, insights at the molecular level can contribute to strategies for malaria prevention. Recent advances in single-cell RNA sequencing provide an opportunity to assess sporozoite heterogeneity at a resolution unattainable by bulk RNA sequencing methods. In this study, we use a droplet-based single-cell RNA sequencing workflow to analyze the transcriptomes of over 8000 Plasmodium berghei sporozoites derived from the midguts and salivary glands of Anopheles stephensi mosquitoes. The detection of known marker genes confirms the successful capture and sequencing of samples composed of a mixed population of sporozoites. Using data integration, clustering, and trajectory analyses, we reveal differences in gene expression profiles of individual sporozoites, and identify both annotated and unannotated markers associated with sporozoite development. Our work highlights the utility of a high-throughput workflow for the transcriptomic profiling of Plasmodium sporozoites, and provides new insights into gene usage during the parasite’s development in the mosquito.
format	article
author	Anthony A. Ruberto Caitlin Bourke Nicolas Merienne Thomas Obadia Rogerio Amino Ivo Mueller
author_facet	Anthony A. Ruberto Caitlin Bourke Nicolas Merienne Thomas Obadia Rogerio Amino Ivo Mueller
author_sort	Anthony A. Ruberto
title	Single-cell RNA sequencing reveals developmental heterogeneity among Plasmodium berghei sporozoites
title_short	Single-cell RNA sequencing reveals developmental heterogeneity among Plasmodium berghei sporozoites
title_full	Single-cell RNA sequencing reveals developmental heterogeneity among Plasmodium berghei sporozoites
title_fullStr	Single-cell RNA sequencing reveals developmental heterogeneity among Plasmodium berghei sporozoites
title_full_unstemmed	Single-cell RNA sequencing reveals developmental heterogeneity among Plasmodium berghei sporozoites
title_sort	single-cell rna sequencing reveals developmental heterogeneity among plasmodium berghei sporozoites
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/3ed21ec8665144f28e647dfbfbc05286
work_keys_str_mv	AT anthonyaruberto singlecellrnasequencingrevealsdevelopmentalheterogeneityamongplasmodiumbergheisporozoites AT caitlinbourke singlecellrnasequencingrevealsdevelopmentalheterogeneityamongplasmodiumbergheisporozoites AT nicolasmerienne singlecellrnasequencingrevealsdevelopmentalheterogeneityamongplasmodiumbergheisporozoites AT thomasobadia singlecellrnasequencingrevealsdevelopmentalheterogeneityamongplasmodiumbergheisporozoites AT rogerioamino singlecellrnasequencingrevealsdevelopmentalheterogeneityamongplasmodiumbergheisporozoites AT ivomueller singlecellrnasequencingrevealsdevelopmentalheterogeneityamongplasmodiumbergheisporozoites
_version_	1718391246249000960

Single-cell RNA sequencing reveals developmental heterogeneity among Plasmodium berghei sporozoites

Ejemplares similares