A cell-free biosynthesis platform for modular construction of protein glycosylation pathways
Constructing biosynthetic pathways to study and engineer glycoprotein structures is difficult. Here, the authors use GlycoPRIME, a cell-free workflow for mixing-and-matching glycosylation enzymes, to evaluate 37 putative glycosylation pathways and discover routes to 18 new glycoprotein structures
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Main Authors: | Weston Kightlinger, Katherine E. Duncker, Ashvita Ramesh, Ariel H. Thames, Aravind Natarajan, Jessica C. Stark, Allen Yang, Liang Lin, Milan Mrksich, Matthew P. DeLisa, Michael C. Jewett |
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Format: | article |
Language: | EN |
Published: |
Nature Portfolio
2019
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Subjects: | |
Online Access: | https://doaj.org/article/3ed28a4885534b4f9dbe3db52b4d222c |
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