A cell-free biosynthesis platform for modular construction of protein glycosylation pathways

Constructing biosynthetic pathways to study and engineer glycoprotein structures is difficult. Here, the authors use GlycoPRIME, a cell-free workflow for mixing-and-matching glycosylation enzymes, to evaluate 37 putative glycosylation pathways and discover routes to 18 new glycoprotein structures

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Auteurs principaux: Weston Kightlinger, Katherine E. Duncker, Ashvita Ramesh, Ariel H. Thames, Aravind Natarajan, Jessica C. Stark, Allen Yang, Liang Lin, Milan Mrksich, Matthew P. DeLisa, Michael C. Jewett
Format: article
Langue:EN
Publié: Nature Portfolio 2019
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Accès en ligne:https://doaj.org/article/3ed28a4885534b4f9dbe3db52b4d222c
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