Methodologies for the Determination of Blood Alpha1 Antitrypsin Levels: A Systematic Review

Background: The study of hematic concentrations of alpha1 antitrypsin (AAT) is currently one step in the diagnosis of AAT deficiency. To try to clarify the relevance of the laboratory techniques, we carried out a systematic review of the literature. Methods: Studies evaluating the quantification of...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Borja Ruiz-Duque, Lucía Bañuls, Rocio Reinoso-Arija, Laura Carrasco-Hernandez, Candelaria Caballero-Eraso, Francisco Dasí, José Luis Lopez-Campos
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	alpha1-antitripsin serum plasma blood concentration nephelometry turbidimetry Medicine R
Acceso en línea:	https://doaj.org/article/3eda1d149b0e4728ab783148257f4fc4
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:3eda1d149b0e4728ab783148257f4fc4
record_format	dspace
spelling	oai:doaj.org-article:3eda1d149b0e4728ab783148257f4fc42021-11-11T17:45:05ZMethodologies for the Determination of Blood Alpha1 Antitrypsin Levels: A Systematic Review10.3390/jcm102151322077-0383https://doaj.org/article/3eda1d149b0e4728ab783148257f4fc42021-10-01T00:00:00Zhttps://www.mdpi.com/2077-0383/10/21/5132https://doaj.org/toc/2077-0383Background: The study of hematic concentrations of alpha1 antitrypsin (AAT) is currently one step in the diagnosis of AAT deficiency. To try to clarify the relevance of the laboratory techniques, we carried out a systematic review of the literature. Methods: Studies evaluating the quantification of AAT in peripheral blood were searched in PubMed in July 2021. The selection criteria included (1) any type of study design that included a quantification of AAT in peripheral blood; (2) studies written in English or Spanish; (3) studies evaluating human beings; and (4) studies involving adults. Results: Out of 207 studies, the most frequently used techniques were nephelometry (43.9%), followed by ELISA (19.8%) and turbidimetry (13.5%). Altogether, 182 (87.9%) cases expressed their results in units of gram, while 16 (7.7%) articles expressed them in units of mole. Only 2.9% articles referred to the standard used, 43.5% articles indicated the commercial kit used, and 36.2% indicated the analyzer used. Conclusions: The technical aspects of these determinations are not always reported in the literature. Journals should be attentive to these technical requirements and ensure that they are included in the works in which AAT is determined in order to ensure a correct interpretation of the study findings.Borja Ruiz-DuqueLucía BañulsRocio Reinoso-ArijaLaura Carrasco-HernandezCandelaria Caballero-ErasoFrancisco DasíJosé Luis Lopez-CamposMDPI AGarticlealpha1-antitripsinserumplasmablood concentrationnephelometryturbidimetryMedicineRENJournal of Clinical Medicine, Vol 10, Iss 5132, p 5132 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	alpha1-antitripsin serum plasma blood concentration nephelometry turbidimetry Medicine R
spellingShingle	alpha1-antitripsin serum plasma blood concentration nephelometry turbidimetry Medicine R Borja Ruiz-Duque Lucía Bañuls Rocio Reinoso-Arija Laura Carrasco-Hernandez Candelaria Caballero-Eraso Francisco Dasí José Luis Lopez-Campos Methodologies for the Determination of Blood Alpha1 Antitrypsin Levels: A Systematic Review
description	Background: The study of hematic concentrations of alpha1 antitrypsin (AAT) is currently one step in the diagnosis of AAT deficiency. To try to clarify the relevance of the laboratory techniques, we carried out a systematic review of the literature. Methods: Studies evaluating the quantification of AAT in peripheral blood were searched in PubMed in July 2021. The selection criteria included (1) any type of study design that included a quantification of AAT in peripheral blood; (2) studies written in English or Spanish; (3) studies evaluating human beings; and (4) studies involving adults. Results: Out of 207 studies, the most frequently used techniques were nephelometry (43.9%), followed by ELISA (19.8%) and turbidimetry (13.5%). Altogether, 182 (87.9%) cases expressed their results in units of gram, while 16 (7.7%) articles expressed them in units of mole. Only 2.9% articles referred to the standard used, 43.5% articles indicated the commercial kit used, and 36.2% indicated the analyzer used. Conclusions: The technical aspects of these determinations are not always reported in the literature. Journals should be attentive to these technical requirements and ensure that they are included in the works in which AAT is determined in order to ensure a correct interpretation of the study findings.
format	article
author	Borja Ruiz-Duque Lucía Bañuls Rocio Reinoso-Arija Laura Carrasco-Hernandez Candelaria Caballero-Eraso Francisco Dasí José Luis Lopez-Campos
author_facet	Borja Ruiz-Duque Lucía Bañuls Rocio Reinoso-Arija Laura Carrasco-Hernandez Candelaria Caballero-Eraso Francisco Dasí José Luis Lopez-Campos
author_sort	Borja Ruiz-Duque
title	Methodologies for the Determination of Blood Alpha1 Antitrypsin Levels: A Systematic Review
title_short	Methodologies for the Determination of Blood Alpha1 Antitrypsin Levels: A Systematic Review
title_full	Methodologies for the Determination of Blood Alpha1 Antitrypsin Levels: A Systematic Review
title_fullStr	Methodologies for the Determination of Blood Alpha1 Antitrypsin Levels: A Systematic Review
title_full_unstemmed	Methodologies for the Determination of Blood Alpha1 Antitrypsin Levels: A Systematic Review
title_sort	methodologies for the determination of blood alpha1 antitrypsin levels: a systematic review
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/3eda1d149b0e4728ab783148257f4fc4
work_keys_str_mv	AT borjaruizduque methodologiesforthedeterminationofbloodalpha1antitrypsinlevelsasystematicreview AT luciabanuls methodologiesforthedeterminationofbloodalpha1antitrypsinlevelsasystematicreview AT rocioreinosoarija methodologiesforthedeterminationofbloodalpha1antitrypsinlevelsasystematicreview AT lauracarrascohernandez methodologiesforthedeterminationofbloodalpha1antitrypsinlevelsasystematicreview AT candelariacaballeroeraso methodologiesforthedeterminationofbloodalpha1antitrypsinlevelsasystematicreview AT franciscodasi methodologiesforthedeterminationofbloodalpha1antitrypsinlevelsasystematicreview AT joseluislopezcampos methodologiesforthedeterminationofbloodalpha1antitrypsinlevelsasystematicreview
_version_	1718431996507586560

Methodologies for the Determination of Blood Alpha1 Antitrypsin Levels: A Systematic Review

Ejemplares similares