Bone marrow niche-mimetics modulate HSPC function via integrin signaling

Abstract The bone marrow (BM) microenvironment provides critical physical cues for hematopoietic stem and progenitor cell (HSPC) maintenance and fate decision mediated by cell-matrix interactions. However, the mechanisms underlying matrix communication and signal transduction are less well understoo...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Martin Kräter, Angela Jacobi, Oliver Otto, Stefanie Tietze, Katrin Müller, David M. Poitz, Sandra Palm, Valentina M. Zinna, Ulrike Biehain, Manja Wobus, Triantafyllos Chavakis, Carsten Werner, Jochen Guck, Martin Bornhauser
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/3edcabb0611b42bd9fb7a863b22e1766
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:3edcabb0611b42bd9fb7a863b22e1766
record_format	dspace
spelling	oai:doaj.org-article:3edcabb0611b42bd9fb7a863b22e17662021-12-02T15:05:06ZBone marrow niche-mimetics modulate HSPC function via integrin signaling10.1038/s41598-017-02352-52045-2322https://doaj.org/article/3edcabb0611b42bd9fb7a863b22e17662017-05-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-02352-5https://doaj.org/toc/2045-2322Abstract The bone marrow (BM) microenvironment provides critical physical cues for hematopoietic stem and progenitor cell (HSPC) maintenance and fate decision mediated by cell-matrix interactions. However, the mechanisms underlying matrix communication and signal transduction are less well understood. Contrary, stem cell culture is mainly facilitated in suspension cultures. Here, we used bone marrow-mimetic decellularized extracellular matrix (ECM) scaffolds derived from mesenchymal stromal cells (MSCs) to study HSPC-ECM interaction. Seeding freshly isolated HSPCs adherent (AT) and non-adherent (SN) cells were found. We detected enhanced expansion and active migration of AT-cells mediated by ECM incorporated stromal derived factor one. Probing cell mechanics, AT-cells displayed naïve cell deformation compared to SN-cells indicating physical recognition of ECM material properties by focal adhesion. Integrin αIIb (CD41), αV (CD51) and β3 (CD61) were found to be induced. Signaling focal contacts via ITGβ3 were identified to facilitate cell adhesion, migration and mediate ECM-physical cues to modulate HSPC function.Martin KräterAngela JacobiOliver OttoStefanie TietzeKatrin MüllerDavid M. PoitzSandra PalmValentina M. ZinnaUlrike BiehainManja WobusTriantafyllos ChavakisCarsten WernerJochen GuckMartin BornhauserNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-15 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Martin Kräter Angela Jacobi Oliver Otto Stefanie Tietze Katrin Müller David M. Poitz Sandra Palm Valentina M. Zinna Ulrike Biehain Manja Wobus Triantafyllos Chavakis Carsten Werner Jochen Guck Martin Bornhauser Bone marrow niche-mimetics modulate HSPC function via integrin signaling
description	Abstract The bone marrow (BM) microenvironment provides critical physical cues for hematopoietic stem and progenitor cell (HSPC) maintenance and fate decision mediated by cell-matrix interactions. However, the mechanisms underlying matrix communication and signal transduction are less well understood. Contrary, stem cell culture is mainly facilitated in suspension cultures. Here, we used bone marrow-mimetic decellularized extracellular matrix (ECM) scaffolds derived from mesenchymal stromal cells (MSCs) to study HSPC-ECM interaction. Seeding freshly isolated HSPCs adherent (AT) and non-adherent (SN) cells were found. We detected enhanced expansion and active migration of AT-cells mediated by ECM incorporated stromal derived factor one. Probing cell mechanics, AT-cells displayed naïve cell deformation compared to SN-cells indicating physical recognition of ECM material properties by focal adhesion. Integrin αIIb (CD41), αV (CD51) and β3 (CD61) were found to be induced. Signaling focal contacts via ITGβ3 were identified to facilitate cell adhesion, migration and mediate ECM-physical cues to modulate HSPC function.
format	article
author	Martin Kräter Angela Jacobi Oliver Otto Stefanie Tietze Katrin Müller David M. Poitz Sandra Palm Valentina M. Zinna Ulrike Biehain Manja Wobus Triantafyllos Chavakis Carsten Werner Jochen Guck Martin Bornhauser
author_facet	Martin Kräter Angela Jacobi Oliver Otto Stefanie Tietze Katrin Müller David M. Poitz Sandra Palm Valentina M. Zinna Ulrike Biehain Manja Wobus Triantafyllos Chavakis Carsten Werner Jochen Guck Martin Bornhauser
author_sort	Martin Kräter
title	Bone marrow niche-mimetics modulate HSPC function via integrin signaling
title_short	Bone marrow niche-mimetics modulate HSPC function via integrin signaling
title_full	Bone marrow niche-mimetics modulate HSPC function via integrin signaling
title_fullStr	Bone marrow niche-mimetics modulate HSPC function via integrin signaling
title_full_unstemmed	Bone marrow niche-mimetics modulate HSPC function via integrin signaling
title_sort	bone marrow niche-mimetics modulate hspc function via integrin signaling
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/3edcabb0611b42bd9fb7a863b22e1766
work_keys_str_mv	AT martinkrater bonemarrownichemimeticsmodulatehspcfunctionviaintegrinsignaling AT angelajacobi bonemarrownichemimeticsmodulatehspcfunctionviaintegrinsignaling AT oliverotto bonemarrownichemimeticsmodulatehspcfunctionviaintegrinsignaling AT stefanietietze bonemarrownichemimeticsmodulatehspcfunctionviaintegrinsignaling AT katrinmuller bonemarrownichemimeticsmodulatehspcfunctionviaintegrinsignaling AT davidmpoitz bonemarrownichemimeticsmodulatehspcfunctionviaintegrinsignaling AT sandrapalm bonemarrownichemimeticsmodulatehspcfunctionviaintegrinsignaling AT valentinamzinna bonemarrownichemimeticsmodulatehspcfunctionviaintegrinsignaling AT ulrikebiehain bonemarrownichemimeticsmodulatehspcfunctionviaintegrinsignaling AT manjawobus bonemarrownichemimeticsmodulatehspcfunctionviaintegrinsignaling AT triantafylloschavakis bonemarrownichemimeticsmodulatehspcfunctionviaintegrinsignaling AT carstenwerner bonemarrownichemimeticsmodulatehspcfunctionviaintegrinsignaling AT jochenguck bonemarrownichemimeticsmodulatehspcfunctionviaintegrinsignaling AT martinbornhauser bonemarrownichemimeticsmodulatehspcfunctionviaintegrinsignaling
_version_	1718388949576056832

Bone marrow niche-mimetics modulate HSPC function via integrin signaling

Ejemplares similares