A High-Density Genetic Linkage Map and Fine Mapping of QTL For Feed Conversion Efficiency in Common Carp (Cyprinus carpio)

Feed conversion efficiency (FCE) is an economically crucial trait in fish, however, little progress has been made in genetics and genomics for this trait because phenotypes of the trait are difficult to measure. In this study, we constructed a high-density and high-resolution genetic linkage map wit...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Xiaofeng Zhang, Peixian Luan, Dingchen Cao, Guo Hu
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	common carp (Cyprinus carpio) genetic linkage map feed conversion efficiency QTL mapping candidate genes Genetics QH426-470
Acceso en línea:	https://doaj.org/article/3ee2bcf53aef4e5497be51c56d415a74
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:3ee2bcf53aef4e5497be51c56d415a74
record_format	dspace
spelling	oai:doaj.org-article:3ee2bcf53aef4e5497be51c56d415a742021-11-12T06:43:43ZA High-Density Genetic Linkage Map and Fine Mapping of QTL For Feed Conversion Efficiency in Common Carp (Cyprinus carpio)1664-802110.3389/fgene.2021.778487https://doaj.org/article/3ee2bcf53aef4e5497be51c56d415a742021-11-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fgene.2021.778487/fullhttps://doaj.org/toc/1664-8021Feed conversion efficiency (FCE) is an economically crucial trait in fish, however, little progress has been made in genetics and genomics for this trait because phenotypes of the trait are difficult to measure. In this study, we constructed a high-density and high-resolution genetic linkage map with 28,416 SNP markers for common carp (Cyprinus carpio) based on high throughput genotyping with the carp 250K single nucleotide polymorphism (SNP) array in a full-sib F1 family of mirror carp (Cyprinus carpio) consisting of 141 progenies. The linkage map contained 11,983 distinct loci and spanned 3,590.09 cM with an average locus interval of 0.33 cM. A total of 17 QTL for the FCE trait were detected on four LGs (LG9, LG20, LG28, and LG32), explaining 8.9–15.9% of the phenotypic variations. One major cluster containing eight QTL (qFCE1-28, qFCE2-28, qFCE3-28, qFCE4-28, qFCE5-28, qFCE6-28, qFCE7-28, and qFCE8-28) was detected on LG28. Two clusters consisting of four QTL (qFCE1-32, qFCE2-32, qFCE3-32, and qFCE4-32) and three QTL (qFCE1-20, qFCE2-20, and qFCE3-20) were detected on LG32 and LG20, respectively. Nine candidate genes (ACACA, SCAF4, SLC2A5, TNMD, PCDH1, FOXO, AGO1, FFAR3, and ARID1A) underlying the feed efficiency trait were also identified, the biological functions of which may be involved in lipid metabolism, carbohydrate metabolism, energy deposition, fat accumulation, digestion, growth regulation, and cell proliferation and differentiation according to GO (Gene Ontology). As an important tool, high-density and high-resolution genetic linkage maps play a crucial role in the QTL fine mapping of economically important traits. Our novel findings provided new insights that elucidate the genetic basis and molecular mechanism of feed efficiency and the subsequent marker-assisted selection breeding in common carp.Xiaofeng ZhangPeixian LuanDingchen CaoGuo HuFrontiers Media S.A.articlecommon carp (Cyprinus carpio)genetic linkage mapfeed conversion efficiencyQTL mappingcandidate genesGeneticsQH426-470ENFrontiers in Genetics, Vol 12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	common carp (Cyprinus carpio) genetic linkage map feed conversion efficiency QTL mapping candidate genes Genetics QH426-470
spellingShingle	common carp (Cyprinus carpio) genetic linkage map feed conversion efficiency QTL mapping candidate genes Genetics QH426-470 Xiaofeng Zhang Peixian Luan Dingchen Cao Guo Hu A High-Density Genetic Linkage Map and Fine Mapping of QTL For Feed Conversion Efficiency in Common Carp (Cyprinus carpio)
description	Feed conversion efficiency (FCE) is an economically crucial trait in fish, however, little progress has been made in genetics and genomics for this trait because phenotypes of the trait are difficult to measure. In this study, we constructed a high-density and high-resolution genetic linkage map with 28,416 SNP markers for common carp (Cyprinus carpio) based on high throughput genotyping with the carp 250K single nucleotide polymorphism (SNP) array in a full-sib F1 family of mirror carp (Cyprinus carpio) consisting of 141 progenies. The linkage map contained 11,983 distinct loci and spanned 3,590.09 cM with an average locus interval of 0.33 cM. A total of 17 QTL for the FCE trait were detected on four LGs (LG9, LG20, LG28, and LG32), explaining 8.9–15.9% of the phenotypic variations. One major cluster containing eight QTL (qFCE1-28, qFCE2-28, qFCE3-28, qFCE4-28, qFCE5-28, qFCE6-28, qFCE7-28, and qFCE8-28) was detected on LG28. Two clusters consisting of four QTL (qFCE1-32, qFCE2-32, qFCE3-32, and qFCE4-32) and three QTL (qFCE1-20, qFCE2-20, and qFCE3-20) were detected on LG32 and LG20, respectively. Nine candidate genes (ACACA, SCAF4, SLC2A5, TNMD, PCDH1, FOXO, AGO1, FFAR3, and ARID1A) underlying the feed efficiency trait were also identified, the biological functions of which may be involved in lipid metabolism, carbohydrate metabolism, energy deposition, fat accumulation, digestion, growth regulation, and cell proliferation and differentiation according to GO (Gene Ontology). As an important tool, high-density and high-resolution genetic linkage maps play a crucial role in the QTL fine mapping of economically important traits. Our novel findings provided new insights that elucidate the genetic basis and molecular mechanism of feed efficiency and the subsequent marker-assisted selection breeding in common carp.
format	article
author	Xiaofeng Zhang Peixian Luan Dingchen Cao Guo Hu
author_facet	Xiaofeng Zhang Peixian Luan Dingchen Cao Guo Hu
author_sort	Xiaofeng Zhang
title	A High-Density Genetic Linkage Map and Fine Mapping of QTL For Feed Conversion Efficiency in Common Carp (Cyprinus carpio)
title_short	A High-Density Genetic Linkage Map and Fine Mapping of QTL For Feed Conversion Efficiency in Common Carp (Cyprinus carpio)
title_full	A High-Density Genetic Linkage Map and Fine Mapping of QTL For Feed Conversion Efficiency in Common Carp (Cyprinus carpio)
title_fullStr	A High-Density Genetic Linkage Map and Fine Mapping of QTL For Feed Conversion Efficiency in Common Carp (Cyprinus carpio)
title_full_unstemmed	A High-Density Genetic Linkage Map and Fine Mapping of QTL For Feed Conversion Efficiency in Common Carp (Cyprinus carpio)
title_sort	high-density genetic linkage map and fine mapping of qtl for feed conversion efficiency in common carp (cyprinus carpio)
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/3ee2bcf53aef4e5497be51c56d415a74
work_keys_str_mv	AT xiaofengzhang ahighdensitygeneticlinkagemapandfinemappingofqtlforfeedconversionefficiencyincommoncarpcyprinuscarpio AT peixianluan ahighdensitygeneticlinkagemapandfinemappingofqtlforfeedconversionefficiencyincommoncarpcyprinuscarpio AT dingchencao ahighdensitygeneticlinkagemapandfinemappingofqtlforfeedconversionefficiencyincommoncarpcyprinuscarpio AT guohu ahighdensitygeneticlinkagemapandfinemappingofqtlforfeedconversionefficiencyincommoncarpcyprinuscarpio AT xiaofengzhang highdensitygeneticlinkagemapandfinemappingofqtlforfeedconversionefficiencyincommoncarpcyprinuscarpio AT peixianluan highdensitygeneticlinkagemapandfinemappingofqtlforfeedconversionefficiencyincommoncarpcyprinuscarpio AT dingchencao highdensitygeneticlinkagemapandfinemappingofqtlforfeedconversionefficiencyincommoncarpcyprinuscarpio AT guohu highdensitygeneticlinkagemapandfinemappingofqtlforfeedconversionefficiencyincommoncarpcyprinuscarpio
_version_	1718431101887709184

A High-Density Genetic Linkage Map and Fine Mapping of QTL For Feed Conversion Efficiency in Common Carp (Cyprinus carpio)

Ejemplares similares