Antioxidant Effect of Montelukast on Acute Lung Injury Induced by Lipopolysaccharide in Dogs

BACKGROUND AND OBJECTIVE: Acute lung injury is characterized by accumulation of neutrophils in the lung, interstitial edema, and damage to the alveolar epithelium. Lipopolysaccharide (LPS) causes an inflammatory response and the release of reactive oxygen species and cellular and tissue damage to th...

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Autores principales:	A Soltanieh, R Avizeh, H Najafzadeh Varzi, M Razi Jalali, M Ghorbanpour
Formato:	article
Lenguaje:	EN FA
Publicado:	Babol University of Medical Sciences 2021
Materias:	acute lung injury oxidative stress dogs lipopolysaccharide montelukast. Medicine R Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/3eeb209fe1974628b822db6afdb23b2c
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spelling	oai:doaj.org-article:3eeb209fe1974628b822db6afdb23b2c2021-11-29T07:40:49ZAntioxidant Effect of Montelukast on Acute Lung Injury Induced by Lipopolysaccharide in Dogs1561-41072251-7170https://doaj.org/article/3eeb209fe1974628b822db6afdb23b2c2021-03-01T00:00:00Zhttp://jbums.org/article-1-9636-en.htmlhttps://doaj.org/toc/1561-4107https://doaj.org/toc/2251-7170BACKGROUND AND OBJECTIVE: Acute lung injury is characterized by accumulation of neutrophils in the lung, interstitial edema, and damage to the alveolar epithelium. Lipopolysaccharide (LPS) causes an inflammatory response and the release of reactive oxygen species and cellular and tissue damage to the lungs. Considering the role of oxidative stress in infections and proving the antioxidant properties of montelukast in several studies, the effect of montelukast on acute lung injury induced by lipopolysaccharide (as a model of infection) in dogs was investigated in this study. METHODS: In this experimental study, 20 healthy dogs (both male and female dogs of native breed with an average weight of 20 kg) were divided into four equal groups. The first group received oral montelukast (1 mg/kg), the second group received intravenous LPS (0.1 µg/kg), the third group received montelukast one hour before LPS and the fourth group received montelukast one hour after LPS. Bronchoalveolar lavage and blood sampling were performed at hour zero and 1.5 hours after the start of the test and the amount of malondialdehyde, catalase activity, glutathione peroxidase and total antioxidant capacity in serum and lavage fluid were measured using a kit. FINDINGS: LPS significantly increased malondialdehyde levels (from 10.5 to 139.8 μmol) and decreased catalase activity (from 0.018 to 0.007 μmol) (p= 0.0001), glutathione peroxidase (from 259 to 76.5 nmol) and the total antioxidant capacity (from 0.41 to 0.04 nmol) compared to hour zero. These changes were significantly adjusted by montelukast (p≤ 0.02). CONCLUSION: The results of this study showed that montelukast can enhance antioxidant defense against acute lung injury induced by LPS.A SoltaniehR AvizehH Najafzadeh VarziM Razi JalaliM GhorbanpourBabol University of Medical Sciencesarticleacute lung injuryoxidative stressdogslipopolysaccharidemontelukast.MedicineRMedicine (General)R5-920ENFAMajallah-i Dānishgāh-i ̒Ulūm-i Pizishkī-i Bābul, Vol 23, Iss 1, Pp 229-235 (2021)
institution	DOAJ
collection	DOAJ
language	EN FA
topic	acute lung injury oxidative stress dogs lipopolysaccharide montelukast. Medicine R Medicine (General) R5-920
spellingShingle	acute lung injury oxidative stress dogs lipopolysaccharide montelukast. Medicine R Medicine (General) R5-920 A Soltanieh R Avizeh H Najafzadeh Varzi M Razi Jalali M Ghorbanpour Antioxidant Effect of Montelukast on Acute Lung Injury Induced by Lipopolysaccharide in Dogs
description	BACKGROUND AND OBJECTIVE: Acute lung injury is characterized by accumulation of neutrophils in the lung, interstitial edema, and damage to the alveolar epithelium. Lipopolysaccharide (LPS) causes an inflammatory response and the release of reactive oxygen species and cellular and tissue damage to the lungs. Considering the role of oxidative stress in infections and proving the antioxidant properties of montelukast in several studies, the effect of montelukast on acute lung injury induced by lipopolysaccharide (as a model of infection) in dogs was investigated in this study. METHODS: In this experimental study, 20 healthy dogs (both male and female dogs of native breed with an average weight of 20 kg) were divided into four equal groups. The first group received oral montelukast (1 mg/kg), the second group received intravenous LPS (0.1 µg/kg), the third group received montelukast one hour before LPS and the fourth group received montelukast one hour after LPS. Bronchoalveolar lavage and blood sampling were performed at hour zero and 1.5 hours after the start of the test and the amount of malondialdehyde, catalase activity, glutathione peroxidase and total antioxidant capacity in serum and lavage fluid were measured using a kit. FINDINGS: LPS significantly increased malondialdehyde levels (from 10.5 to 139.8 μmol) and decreased catalase activity (from 0.018 to 0.007 μmol) (p= 0.0001), glutathione peroxidase (from 259 to 76.5 nmol) and the total antioxidant capacity (from 0.41 to 0.04 nmol) compared to hour zero. These changes were significantly adjusted by montelukast (p≤ 0.02). CONCLUSION: The results of this study showed that montelukast can enhance antioxidant defense against acute lung injury induced by LPS.
format	article
author	A Soltanieh R Avizeh H Najafzadeh Varzi M Razi Jalali M Ghorbanpour
author_facet	A Soltanieh R Avizeh H Najafzadeh Varzi M Razi Jalali M Ghorbanpour
author_sort	A Soltanieh
title	Antioxidant Effect of Montelukast on Acute Lung Injury Induced by Lipopolysaccharide in Dogs
title_short	Antioxidant Effect of Montelukast on Acute Lung Injury Induced by Lipopolysaccharide in Dogs
title_full	Antioxidant Effect of Montelukast on Acute Lung Injury Induced by Lipopolysaccharide in Dogs
title_fullStr	Antioxidant Effect of Montelukast on Acute Lung Injury Induced by Lipopolysaccharide in Dogs
title_full_unstemmed	Antioxidant Effect of Montelukast on Acute Lung Injury Induced by Lipopolysaccharide in Dogs
title_sort	antioxidant effect of montelukast on acute lung injury induced by lipopolysaccharide in dogs
publisher	Babol University of Medical Sciences
publishDate	2021
url	https://doaj.org/article/3eeb209fe1974628b822db6afdb23b2c
work_keys_str_mv	AT asoltanieh antioxidanteffectofmontelukastonacutelunginjuryinducedbylipopolysaccharideindogs AT ravizeh antioxidanteffectofmontelukastonacutelunginjuryinducedbylipopolysaccharideindogs AT hnajafzadehvarzi antioxidanteffectofmontelukastonacutelunginjuryinducedbylipopolysaccharideindogs AT mrazijalali antioxidanteffectofmontelukastonacutelunginjuryinducedbylipopolysaccharideindogs AT mghorbanpour antioxidanteffectofmontelukastonacutelunginjuryinducedbylipopolysaccharideindogs
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Antioxidant Effect of Montelukast on Acute Lung Injury Induced by Lipopolysaccharide in Dogs

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