Blood bank storage of red blood cells increases RBC cytoplasmic membrane order and bending rigidity

Blood banks around the world store blood components for several weeks ensuring its availability for transfusion medicine. Red blood cells (RBCs) are known to undergo compositional changes during storage, which may impact the cells’ function and eventually the recipients’ health. We extracted the RBC...

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Autores principales:	Sebastian Himbert, Syed M. Qadri, William P. Sheffield, Peter Schubert, Angelo D’Alessandro, Maikel C. Rheinstädter
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/3ef5864f252f4669bdab478548b1ed83
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spelling	oai:doaj.org-article:3ef5864f252f4669bdab478548b1ed832021-11-25T06:11:03ZBlood bank storage of red blood cells increases RBC cytoplasmic membrane order and bending rigidity1932-6203https://doaj.org/article/3ef5864f252f4669bdab478548b1ed832021-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC8589153/?tool=EBIhttps://doaj.org/toc/1932-6203Blood banks around the world store blood components for several weeks ensuring its availability for transfusion medicine. Red blood cells (RBCs) are known to undergo compositional changes during storage, which may impact the cells’ function and eventually the recipients’ health. We extracted the RBC’s cytoplasmic membrane (RBCcm) to study the effect of storage on the membranes’ molecular structure and bending rigidity by a combination of X-ray diffraction (XRD), X-ray diffuse scattering (XDS) and coarse grained Molecular Dynamics (MD) simulations. Blood was stored in commercial blood bags for 2 and 5 weeks, respectively and compared to freshly drawn blood. Using mass spectrometry, we measured an increase of fatty acids together with a slight shift towards shorter tail lengths. We observe an increased fraction (6%) of liquid ordered (lo) domains in the RBCcms with storage time, and an increased lipid packing in these domains, leading to an increased membrane thickness and membrane order. The size of both, lo and liquid disordered (ld) lipid domains was found to decrease with increased storage time by up to 25%. XDS experiments reveal a storage dependent increase in the RBCcm’s bending modulus κ by a factor of 2.8, from 1.9 kBT to 5.3 kBT. MD simulations were conducted in the absence of proteins. The results show that the membrane composition has a small contribution to the increased bending rigidity and suggests additional protein-driven mechanisms.Sebastian HimbertSyed M. QadriWilliam P. SheffieldPeter SchubertAngelo D’AlessandroMaikel C. RheinstädterPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 16, Iss 11 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Sebastian Himbert Syed M. Qadri William P. Sheffield Peter Schubert Angelo D’Alessandro Maikel C. Rheinstädter Blood bank storage of red blood cells increases RBC cytoplasmic membrane order and bending rigidity
description	Blood banks around the world store blood components for several weeks ensuring its availability for transfusion medicine. Red blood cells (RBCs) are known to undergo compositional changes during storage, which may impact the cells’ function and eventually the recipients’ health. We extracted the RBC’s cytoplasmic membrane (RBCcm) to study the effect of storage on the membranes’ molecular structure and bending rigidity by a combination of X-ray diffraction (XRD), X-ray diffuse scattering (XDS) and coarse grained Molecular Dynamics (MD) simulations. Blood was stored in commercial blood bags for 2 and 5 weeks, respectively and compared to freshly drawn blood. Using mass spectrometry, we measured an increase of fatty acids together with a slight shift towards shorter tail lengths. We observe an increased fraction (6%) of liquid ordered (lo) domains in the RBCcms with storage time, and an increased lipid packing in these domains, leading to an increased membrane thickness and membrane order. The size of both, lo and liquid disordered (ld) lipid domains was found to decrease with increased storage time by up to 25%. XDS experiments reveal a storage dependent increase in the RBCcm’s bending modulus κ by a factor of 2.8, from 1.9 kBT to 5.3 kBT. MD simulations were conducted in the absence of proteins. The results show that the membrane composition has a small contribution to the increased bending rigidity and suggests additional protein-driven mechanisms.
format	article
author	Sebastian Himbert Syed M. Qadri William P. Sheffield Peter Schubert Angelo D’Alessandro Maikel C. Rheinstädter
author_facet	Sebastian Himbert Syed M. Qadri William P. Sheffield Peter Schubert Angelo D’Alessandro Maikel C. Rheinstädter
author_sort	Sebastian Himbert
title	Blood bank storage of red blood cells increases RBC cytoplasmic membrane order and bending rigidity
title_short	Blood bank storage of red blood cells increases RBC cytoplasmic membrane order and bending rigidity
title_full	Blood bank storage of red blood cells increases RBC cytoplasmic membrane order and bending rigidity
title_fullStr	Blood bank storage of red blood cells increases RBC cytoplasmic membrane order and bending rigidity
title_full_unstemmed	Blood bank storage of red blood cells increases RBC cytoplasmic membrane order and bending rigidity
title_sort	blood bank storage of red blood cells increases rbc cytoplasmic membrane order and bending rigidity
publisher	Public Library of Science (PLoS)
publishDate	2021
url	https://doaj.org/article/3ef5864f252f4669bdab478548b1ed83
work_keys_str_mv	AT sebastianhimbert bloodbankstorageofredbloodcellsincreasesrbccytoplasmicmembraneorderandbendingrigidity AT syedmqadri bloodbankstorageofredbloodcellsincreasesrbccytoplasmicmembraneorderandbendingrigidity AT williampsheffield bloodbankstorageofredbloodcellsincreasesrbccytoplasmicmembraneorderandbendingrigidity AT peterschubert bloodbankstorageofredbloodcellsincreasesrbccytoplasmicmembraneorderandbendingrigidity AT angelodalessandro bloodbankstorageofredbloodcellsincreasesrbccytoplasmicmembraneorderandbendingrigidity AT maikelcrheinstadter bloodbankstorageofredbloodcellsincreasesrbccytoplasmicmembraneorderandbendingrigidity
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Blood bank storage of red blood cells increases RBC cytoplasmic membrane order and bending rigidity

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