Enhanced production of docosahexaenoic acid in mammalian cells.

Docosahexaenoic acid (DHA), one of the important polyunsaturated fatty acids (PUFA) with pharmaceutical and nutraceutical effects, may be obtained through diet or synthesized in vivo from dietary a-linolenic acid (ALA). However, the accumulation of DHA in human body or other mammals relies on the in...

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Autores principales:	Guiming Zhu, Xudong Jiang, Qin Ou, Tao Zhang, Mingfu Wang, Guozhi Sun, Zhao Wang, Jie Sun, Tangdong Ge
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2014
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/3ef61c501c1c45d4b6dc9b6b1eadbbf9
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spelling	oai:doaj.org-article:3ef61c501c1c45d4b6dc9b6b1eadbbf92021-11-18T08:21:03ZEnhanced production of docosahexaenoic acid in mammalian cells.1932-620310.1371/journal.pone.0096503https://doaj.org/article/3ef61c501c1c45d4b6dc9b6b1eadbbf92014-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24788769/?tool=EBIhttps://doaj.org/toc/1932-6203Docosahexaenoic acid (DHA), one of the important polyunsaturated fatty acids (PUFA) with pharmaceutical and nutraceutical effects, may be obtained through diet or synthesized in vivo from dietary a-linolenic acid (ALA). However, the accumulation of DHA in human body or other mammals relies on the intake of high dose of DHA for a certain period of time, and the bioconversion of dietary ALA to DHA is very limited. Therefore the mammalian cells are not rich in DHA. Here, we report a new technology for increased production of DHA in mammalian cells. By using transient transfection method, Siganus canaliculatus Δ4 desaturase was heterologously expressed in chinese hamster ovary (CHO) cells, and simultaneously, mouse Δ6-desaturase and Δ5-desaturase were overexpressed. The results demonstrated that the overexpression of Δ6/Δ5-desaturases significantly enhanced the ability of transfected cells to convert the added ALA to docosapentaenoic acid (DPA) which in turn get converted into DHA directly and efficiently by the heterologously expressed Δ4 desaturase. This technology provides the basis for potential utility of these gene constructs in the creation of transgenic livestock for increased production of DHA/related products to meet the growing demand of this important PUFA.Guiming ZhuXudong JiangQin OuTao ZhangMingfu WangGuozhi SunZhao WangJie SunTangdong GePublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 9, Iss 5, p e96503 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Guiming Zhu Xudong Jiang Qin Ou Tao Zhang Mingfu Wang Guozhi Sun Zhao Wang Jie Sun Tangdong Ge Enhanced production of docosahexaenoic acid in mammalian cells.
description	Docosahexaenoic acid (DHA), one of the important polyunsaturated fatty acids (PUFA) with pharmaceutical and nutraceutical effects, may be obtained through diet or synthesized in vivo from dietary a-linolenic acid (ALA). However, the accumulation of DHA in human body or other mammals relies on the intake of high dose of DHA for a certain period of time, and the bioconversion of dietary ALA to DHA is very limited. Therefore the mammalian cells are not rich in DHA. Here, we report a new technology for increased production of DHA in mammalian cells. By using transient transfection method, Siganus canaliculatus Δ4 desaturase was heterologously expressed in chinese hamster ovary (CHO) cells, and simultaneously, mouse Δ6-desaturase and Δ5-desaturase were overexpressed. The results demonstrated that the overexpression of Δ6/Δ5-desaturases significantly enhanced the ability of transfected cells to convert the added ALA to docosapentaenoic acid (DPA) which in turn get converted into DHA directly and efficiently by the heterologously expressed Δ4 desaturase. This technology provides the basis for potential utility of these gene constructs in the creation of transgenic livestock for increased production of DHA/related products to meet the growing demand of this important PUFA.
format	article
author	Guiming Zhu Xudong Jiang Qin Ou Tao Zhang Mingfu Wang Guozhi Sun Zhao Wang Jie Sun Tangdong Ge
author_facet	Guiming Zhu Xudong Jiang Qin Ou Tao Zhang Mingfu Wang Guozhi Sun Zhao Wang Jie Sun Tangdong Ge
author_sort	Guiming Zhu
title	Enhanced production of docosahexaenoic acid in mammalian cells.
title_short	Enhanced production of docosahexaenoic acid in mammalian cells.
title_full	Enhanced production of docosahexaenoic acid in mammalian cells.
title_fullStr	Enhanced production of docosahexaenoic acid in mammalian cells.
title_full_unstemmed	Enhanced production of docosahexaenoic acid in mammalian cells.
title_sort	enhanced production of docosahexaenoic acid in mammalian cells.
publisher	Public Library of Science (PLoS)
publishDate	2014
url	https://doaj.org/article/3ef61c501c1c45d4b6dc9b6b1eadbbf9
work_keys_str_mv	AT guimingzhu enhancedproductionofdocosahexaenoicacidinmammaliancells AT xudongjiang enhancedproductionofdocosahexaenoicacidinmammaliancells AT qinou enhancedproductionofdocosahexaenoicacidinmammaliancells AT taozhang enhancedproductionofdocosahexaenoicacidinmammaliancells AT mingfuwang enhancedproductionofdocosahexaenoicacidinmammaliancells AT guozhisun enhancedproductionofdocosahexaenoicacidinmammaliancells AT zhaowang enhancedproductionofdocosahexaenoicacidinmammaliancells AT jiesun enhancedproductionofdocosahexaenoicacidinmammaliancells AT tangdongge enhancedproductionofdocosahexaenoicacidinmammaliancells
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Enhanced production of docosahexaenoic acid in mammalian cells.

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