Acid bone lysate activates TGFβ signalling in human oral fibroblasts
Abstract Demineralized bone matrix is a widely used allograft from which not only the inorganic mineral but also embedded growth factors are removed by hydrochloric acid (HCl). The cellular response to the growth factors released during the preparation of demineralized bone matrix, however, has not...
Guardado en:
| Autores principales: | , , , , , , , |
|---|---|
| Formato: | article |
| Lenguaje: | EN |
| Publicado: |
Nature Portfolio
2018
|
| Materias: | |
| Acceso en línea: | https://doaj.org/article/3ef9076c999c4b7e9dda1cd894339262 |
| Etiquetas: |
Agregar Etiqueta
Sin Etiquetas, Sea el primero en etiquetar este registro!
|
| id |
oai:doaj.org-article:3ef9076c999c4b7e9dda1cd894339262 |
|---|---|
| record_format |
dspace |
| spelling |
oai:doaj.org-article:3ef9076c999c4b7e9dda1cd8943392622021-12-02T15:08:36ZAcid bone lysate activates TGFβ signalling in human oral fibroblasts10.1038/s41598-018-34418-32045-2322https://doaj.org/article/3ef9076c999c4b7e9dda1cd8943392622018-10-01T00:00:00Zhttps://doi.org/10.1038/s41598-018-34418-3https://doaj.org/toc/2045-2322Abstract Demineralized bone matrix is a widely used allograft from which not only the inorganic mineral but also embedded growth factors are removed by hydrochloric acid (HCl). The cellular response to the growth factors released during the preparation of demineralized bone matrix, however, has not been studied. Here we investigated the in vitro impact of acid bone lysate (ABL) prepared from porcine cortical bone chips on oral fibroblasts. Proteomic analysis of ABL revealed a large spectrum of bone-derived proteins including TGF-β1. Whole genome microarrays and RT-PCR together with the pharmacologic blocking of TGF-β receptor type I kinase with SB431542 showed that ABL activates the TGF-β target genes interleukin 11, proteoglycan 4, and NADPH oxidase 4. Interleukin 11 expression was confirmed at the protein level by ELISA. Immunofluorescence and Western blot showed the nuclear localization of Smad2/3 and increased phosphorylation of Smad3 with ABL, respectively. This effect was independent of whether ABL was prepared from mandible, calvaria or tibia. These results demonstrate that TGF-β is a major growth factor that is removed upon the preparation of demineralized bone matrix.Franz Josef StraussAlexandra StähliLucian BeerGoran MitulovićValentina GilmozziNina HaspelGerhild SchwabReinhard GruberNature PortfolioarticleOral FibroblastsTGFβ SignalingDemineralized Bone MatrixNADPH Oxidase (NOX4)Bone ChipsMedicineRScienceQENScientific Reports, Vol 8, Iss 1, Pp 1-12 (2018) |
| institution |
DOAJ |
| collection |
DOAJ |
| language |
EN |
| topic |
Oral Fibroblasts TGFβ Signaling Demineralized Bone Matrix NADPH Oxidase (NOX4) Bone Chips Medicine R Science Q |
| spellingShingle |
Oral Fibroblasts TGFβ Signaling Demineralized Bone Matrix NADPH Oxidase (NOX4) Bone Chips Medicine R Science Q Franz Josef Strauss Alexandra Stähli Lucian Beer Goran Mitulović Valentina Gilmozzi Nina Haspel Gerhild Schwab Reinhard Gruber Acid bone lysate activates TGFβ signalling in human oral fibroblasts |
| description |
Abstract Demineralized bone matrix is a widely used allograft from which not only the inorganic mineral but also embedded growth factors are removed by hydrochloric acid (HCl). The cellular response to the growth factors released during the preparation of demineralized bone matrix, however, has not been studied. Here we investigated the in vitro impact of acid bone lysate (ABL) prepared from porcine cortical bone chips on oral fibroblasts. Proteomic analysis of ABL revealed a large spectrum of bone-derived proteins including TGF-β1. Whole genome microarrays and RT-PCR together with the pharmacologic blocking of TGF-β receptor type I kinase with SB431542 showed that ABL activates the TGF-β target genes interleukin 11, proteoglycan 4, and NADPH oxidase 4. Interleukin 11 expression was confirmed at the protein level by ELISA. Immunofluorescence and Western blot showed the nuclear localization of Smad2/3 and increased phosphorylation of Smad3 with ABL, respectively. This effect was independent of whether ABL was prepared from mandible, calvaria or tibia. These results demonstrate that TGF-β is a major growth factor that is removed upon the preparation of demineralized bone matrix. |
| format |
article |
| author |
Franz Josef Strauss Alexandra Stähli Lucian Beer Goran Mitulović Valentina Gilmozzi Nina Haspel Gerhild Schwab Reinhard Gruber |
| author_facet |
Franz Josef Strauss Alexandra Stähli Lucian Beer Goran Mitulović Valentina Gilmozzi Nina Haspel Gerhild Schwab Reinhard Gruber |
| author_sort |
Franz Josef Strauss |
| title |
Acid bone lysate activates TGFβ signalling in human oral fibroblasts |
| title_short |
Acid bone lysate activates TGFβ signalling in human oral fibroblasts |
| title_full |
Acid bone lysate activates TGFβ signalling in human oral fibroblasts |
| title_fullStr |
Acid bone lysate activates TGFβ signalling in human oral fibroblasts |
| title_full_unstemmed |
Acid bone lysate activates TGFβ signalling in human oral fibroblasts |
| title_sort |
acid bone lysate activates tgfβ signalling in human oral fibroblasts |
| publisher |
Nature Portfolio |
| publishDate |
2018 |
| url |
https://doaj.org/article/3ef9076c999c4b7e9dda1cd894339262 |
| work_keys_str_mv |
AT franzjosefstrauss acidbonelysateactivatestgfbsignallinginhumanoralfibroblasts AT alexandrastahli acidbonelysateactivatestgfbsignallinginhumanoralfibroblasts AT lucianbeer acidbonelysateactivatestgfbsignallinginhumanoralfibroblasts AT goranmitulovic acidbonelysateactivatestgfbsignallinginhumanoralfibroblasts AT valentinagilmozzi acidbonelysateactivatestgfbsignallinginhumanoralfibroblasts AT ninahaspel acidbonelysateactivatestgfbsignallinginhumanoralfibroblasts AT gerhildschwab acidbonelysateactivatestgfbsignallinginhumanoralfibroblasts AT reinhardgruber acidbonelysateactivatestgfbsignallinginhumanoralfibroblasts |
| _version_ |
1718388054630072320 |