The role of autophagy in the process of osseointegration around titanium implants with micro-nano topography promoted by osteoimmunity

Abstract Osteoimmunity plays an important role in the process of implant osseointegration. Autophagy is a conservative metabolic pathway of eukaryotic cells, but whether the interaction between autophagy and osteoimmunity plays a key role in osseointegration remains unclear. In this study, we prepar...

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Autores principales:	Ting Zhang, Mengyang Jiang, Xiaojie Yin, Peng Yao, Huiqiang Sun
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/3f033d0ab6d644b0905dc0797d574fb5
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spelling	oai:doaj.org-article:3f033d0ab6d644b0905dc0797d574fb52021-12-02T15:15:58ZThe role of autophagy in the process of osseointegration around titanium implants with micro-nano topography promoted by osteoimmunity10.1038/s41598-021-98007-72045-2322https://doaj.org/article/3f033d0ab6d644b0905dc0797d574fb52021-09-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-98007-7https://doaj.org/toc/2045-2322Abstract Osteoimmunity plays an important role in the process of implant osseointegration. Autophagy is a conservative metabolic pathway of eukaryotic cells, but whether the interaction between autophagy and osteoimmunity plays a key role in osseointegration remains unclear. In this study, we prepared smooth titanium disks and micro-nano topography titanium disks, to study the immune microenvironment of RAW264.7 cells, and prepared the conditioned medium to study the effect of immune microenvironment on the osteogenesis and autophagy of MC3T3-E1 cells. Autophagy inhibitor 3-MA was used to inhibit autophagy to observe the change of expression of osteogenic markers. The results showed that the micro-nano topography titanium disks could stimulate RAW264.7 cells to differentiate into M2 type, forming an anti-inflammatory immune microenvironment; compared with the control group, the anti-inflammatory immune microenvironment promoted the proliferation and differentiation of osteoblasts better. The anti-inflammatory immune environment activated the autophagy level of osteoblasts, while the expression of osteogenic markers was down-regulated after inhibition of autophagy. These results indicate that anti-inflammatory immune microenvironment can promote cell proliferation and osteogenic differentiation, autophagy plays an important role in this process. This study further explains the mechanism of implant osseointegration in osteoimmune microenvironment, and provides reference for improving implant osseointegration.Ting ZhangMengyang JiangXiaojie YinPeng YaoHuiqiang SunNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-11 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Ting Zhang Mengyang Jiang Xiaojie Yin Peng Yao Huiqiang Sun The role of autophagy in the process of osseointegration around titanium implants with micro-nano topography promoted by osteoimmunity
description	Abstract Osteoimmunity plays an important role in the process of implant osseointegration. Autophagy is a conservative metabolic pathway of eukaryotic cells, but whether the interaction between autophagy and osteoimmunity plays a key role in osseointegration remains unclear. In this study, we prepared smooth titanium disks and micro-nano topography titanium disks, to study the immune microenvironment of RAW264.7 cells, and prepared the conditioned medium to study the effect of immune microenvironment on the osteogenesis and autophagy of MC3T3-E1 cells. Autophagy inhibitor 3-MA was used to inhibit autophagy to observe the change of expression of osteogenic markers. The results showed that the micro-nano topography titanium disks could stimulate RAW264.7 cells to differentiate into M2 type, forming an anti-inflammatory immune microenvironment; compared with the control group, the anti-inflammatory immune microenvironment promoted the proliferation and differentiation of osteoblasts better. The anti-inflammatory immune environment activated the autophagy level of osteoblasts, while the expression of osteogenic markers was down-regulated after inhibition of autophagy. These results indicate that anti-inflammatory immune microenvironment can promote cell proliferation and osteogenic differentiation, autophagy plays an important role in this process. This study further explains the mechanism of implant osseointegration in osteoimmune microenvironment, and provides reference for improving implant osseointegration.
format	article
author	Ting Zhang Mengyang Jiang Xiaojie Yin Peng Yao Huiqiang Sun
author_facet	Ting Zhang Mengyang Jiang Xiaojie Yin Peng Yao Huiqiang Sun
author_sort	Ting Zhang
title	The role of autophagy in the process of osseointegration around titanium implants with micro-nano topography promoted by osteoimmunity
title_short	The role of autophagy in the process of osseointegration around titanium implants with micro-nano topography promoted by osteoimmunity
title_full	The role of autophagy in the process of osseointegration around titanium implants with micro-nano topography promoted by osteoimmunity
title_fullStr	The role of autophagy in the process of osseointegration around titanium implants with micro-nano topography promoted by osteoimmunity
title_full_unstemmed	The role of autophagy in the process of osseointegration around titanium implants with micro-nano topography promoted by osteoimmunity
title_sort	role of autophagy in the process of osseointegration around titanium implants with micro-nano topography promoted by osteoimmunity
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/3f033d0ab6d644b0905dc0797d574fb5
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The role of autophagy in the process of osseointegration around titanium implants with micro-nano topography promoted by osteoimmunity

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