3D Computational Modeling of Bleb Initiation Dynamics

Blebbing occurs in cells under high cortical tension when the membrane locally detaches from the actin cortex, resulting in pressure-driven flow of the cytosol and membrane expansion. Some cells use blebs as leading edge protrusions during cell migration, particularly in 3D environments such as a co...

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Autor principal:	Wanda Strychalski
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	cytoplasm biomechanics blebs poroelasticity fluid-structure interaction Physics QC1-999
Acceso en línea:	https://doaj.org/article/3f037cc740104a43b604a65e58f7947d
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spelling	oai:doaj.org-article:3f037cc740104a43b604a65e58f7947d2021-11-30T18:27:33Z3D Computational Modeling of Bleb Initiation Dynamics2296-424X10.3389/fphy.2021.775465https://doaj.org/article/3f037cc740104a43b604a65e58f7947d2021-11-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fphy.2021.775465/fullhttps://doaj.org/toc/2296-424XBlebbing occurs in cells under high cortical tension when the membrane locally detaches from the actin cortex, resulting in pressure-driven flow of the cytosol and membrane expansion. Some cells use blebs as leading edge protrusions during cell migration, particularly in 3D environments such as a collagen matrix. Blebs can be initiated through either a localized loss of membrane-cortex adhesion or ablation of the cortex in a region. Bleb morphologies resulting from different initiation mechanisms have not been studied in detail, either experimentally or with theoretical models. Additionally, material properties of the cytoplasm, such as elasticity, have been shown to be important for limiting bleb size. A 3D dynamic computational model of the cell is presented that includes mechanics and the interactions of the cytoplasm, the actin cortex, the cell membrane, and the cytoskeleton. The model is used to quantify bleb expansion dynamics and shapes that result from simulations using different initiation mechanisms. The cytoplasm is modeled as a both viscous fluid and as a poroelastic material. Results from model simulations with a viscous fluid cytoplasm model show much broader blebs that expand faster when they are initiated via cortical ablation than when they are initiated by removing only membrane-cortex adhesion. Simulation results using the poroelastic model of the cytoplasm provide qualitatively similar bleb morphologies regardless of the initiation mechanism. Parameter studies on bleb expansion time, cytoplasmic stiffness, and permeability reveal different scaling properties, namely a smaller power-law exponent, in 3D simulations compared to 2D ones.Wanda StrychalskiFrontiers Media S.A.articlecytoplasmbiomechanicsblebsporoelasticityfluid-structure interactionPhysicsQC1-999ENFrontiers in Physics, Vol 9 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	cytoplasm biomechanics blebs poroelasticity fluid-structure interaction Physics QC1-999
spellingShingle	cytoplasm biomechanics blebs poroelasticity fluid-structure interaction Physics QC1-999 Wanda Strychalski 3D Computational Modeling of Bleb Initiation Dynamics
description	Blebbing occurs in cells under high cortical tension when the membrane locally detaches from the actin cortex, resulting in pressure-driven flow of the cytosol and membrane expansion. Some cells use blebs as leading edge protrusions during cell migration, particularly in 3D environments such as a collagen matrix. Blebs can be initiated through either a localized loss of membrane-cortex adhesion or ablation of the cortex in a region. Bleb morphologies resulting from different initiation mechanisms have not been studied in detail, either experimentally or with theoretical models. Additionally, material properties of the cytoplasm, such as elasticity, have been shown to be important for limiting bleb size. A 3D dynamic computational model of the cell is presented that includes mechanics and the interactions of the cytoplasm, the actin cortex, the cell membrane, and the cytoskeleton. The model is used to quantify bleb expansion dynamics and shapes that result from simulations using different initiation mechanisms. The cytoplasm is modeled as a both viscous fluid and as a poroelastic material. Results from model simulations with a viscous fluid cytoplasm model show much broader blebs that expand faster when they are initiated via cortical ablation than when they are initiated by removing only membrane-cortex adhesion. Simulation results using the poroelastic model of the cytoplasm provide qualitatively similar bleb morphologies regardless of the initiation mechanism. Parameter studies on bleb expansion time, cytoplasmic stiffness, and permeability reveal different scaling properties, namely a smaller power-law exponent, in 3D simulations compared to 2D ones.
format	article
author	Wanda Strychalski
author_facet	Wanda Strychalski
author_sort	Wanda Strychalski
title	3D Computational Modeling of Bleb Initiation Dynamics
title_short	3D Computational Modeling of Bleb Initiation Dynamics
title_full	3D Computational Modeling of Bleb Initiation Dynamics
title_fullStr	3D Computational Modeling of Bleb Initiation Dynamics
title_full_unstemmed	3D Computational Modeling of Bleb Initiation Dynamics
title_sort	3d computational modeling of bleb initiation dynamics
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/3f037cc740104a43b604a65e58f7947d
work_keys_str_mv	AT wandastrychalski 3dcomputationalmodelingofblebinitiationdynamics
_version_	1718406373391204352

3D Computational Modeling of Bleb Initiation Dynamics

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