Implementation of the asparaginase activity assessment technique for clinical use: experience of a Brazilian Center

Abstract Acute lymphoid leukemia is a childhood cancer that in high-income countries has event-free survival rates of 80% and global survival rates of 90%. In Brazil these rates are under 70%. This difference may be due to the implementation of supportive care, including the assessment of asparagina...

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Autores principales:	Daiane Keller Cecconello, Ciliana Rechenmacher, Isabel Werlang, Priscila Pini Zenatti, Jose Andres Yunes, Ana Paula Alegretti, Claudia Lanvers-Kaminsky, Liane Esteves Daudt, Mariana Bohns Michalowski
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2020
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/3f0393b11e36490cb2ac7fd60d863415
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spelling	oai:doaj.org-article:3f0393b11e36490cb2ac7fd60d8634152021-12-02T12:33:15ZImplementation of the asparaginase activity assessment technique for clinical use: experience of a Brazilian Center10.1038/s41598-020-78549-y2045-2322https://doaj.org/article/3f0393b11e36490cb2ac7fd60d8634152020-12-01T00:00:00Zhttps://doi.org/10.1038/s41598-020-78549-yhttps://doaj.org/toc/2045-2322Abstract Acute lymphoid leukemia is a childhood cancer that in high-income countries has event-free survival rates of 80% and global survival rates of 90%. In Brazil these rates are under 70%. This difference may be due to the implementation of supportive care, including the assessment of asparaginase (ASNase) activity. ASNase may cause hypersensitivity reactions and silent drug inactivation. For this reason, ASNase activity monitoring is an essential tool to ensure an effective treatment. Our aim was to implement an ASNase activity measurement technique at a hospital setting. samples from children who were given Escherichia coli-derived ASNase were collected. The results of the analyses conducted in our laboratory Hospital de Clínicas de Porto Alegre were compared to those of two institutions: Centro Infantil Boldrini and University of Munster. 262 samples were assessed. The results of the first analyses were compared with those obtained at Centro Infantil Boldrini and showed an ICC of 0.954. Thirty samples were sent to the University of Munster and presented an ICC was 0.960. Our results, when compared to those of national and international centers, showed an excellent agreement. The study was able to implement an ASNase activity test to monitor the treatment.Daiane Keller CecconelloCiliana RechenmacherIsabel WerlangPriscila Pini ZenattiJose Andres YunesAna Paula AlegrettiClaudia Lanvers-KaminskyLiane Esteves DaudtMariana Bohns MichalowskiNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 10, Iss 1, Pp 1-6 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Daiane Keller Cecconello Ciliana Rechenmacher Isabel Werlang Priscila Pini Zenatti Jose Andres Yunes Ana Paula Alegretti Claudia Lanvers-Kaminsky Liane Esteves Daudt Mariana Bohns Michalowski Implementation of the asparaginase activity assessment technique for clinical use: experience of a Brazilian Center
description	Abstract Acute lymphoid leukemia is a childhood cancer that in high-income countries has event-free survival rates of 80% and global survival rates of 90%. In Brazil these rates are under 70%. This difference may be due to the implementation of supportive care, including the assessment of asparaginase (ASNase) activity. ASNase may cause hypersensitivity reactions and silent drug inactivation. For this reason, ASNase activity monitoring is an essential tool to ensure an effective treatment. Our aim was to implement an ASNase activity measurement technique at a hospital setting. samples from children who were given Escherichia coli-derived ASNase were collected. The results of the analyses conducted in our laboratory Hospital de Clínicas de Porto Alegre were compared to those of two institutions: Centro Infantil Boldrini and University of Munster. 262 samples were assessed. The results of the first analyses were compared with those obtained at Centro Infantil Boldrini and showed an ICC of 0.954. Thirty samples were sent to the University of Munster and presented an ICC was 0.960. Our results, when compared to those of national and international centers, showed an excellent agreement. The study was able to implement an ASNase activity test to monitor the treatment.
format	article
author	Daiane Keller Cecconello Ciliana Rechenmacher Isabel Werlang Priscila Pini Zenatti Jose Andres Yunes Ana Paula Alegretti Claudia Lanvers-Kaminsky Liane Esteves Daudt Mariana Bohns Michalowski
author_facet	Daiane Keller Cecconello Ciliana Rechenmacher Isabel Werlang Priscila Pini Zenatti Jose Andres Yunes Ana Paula Alegretti Claudia Lanvers-Kaminsky Liane Esteves Daudt Mariana Bohns Michalowski
author_sort	Daiane Keller Cecconello
title	Implementation of the asparaginase activity assessment technique for clinical use: experience of a Brazilian Center
title_short	Implementation of the asparaginase activity assessment technique for clinical use: experience of a Brazilian Center
title_full	Implementation of the asparaginase activity assessment technique for clinical use: experience of a Brazilian Center
title_fullStr	Implementation of the asparaginase activity assessment technique for clinical use: experience of a Brazilian Center
title_full_unstemmed	Implementation of the asparaginase activity assessment technique for clinical use: experience of a Brazilian Center
title_sort	implementation of the asparaginase activity assessment technique for clinical use: experience of a brazilian center
publisher	Nature Portfolio
publishDate	2020
url	https://doaj.org/article/3f0393b11e36490cb2ac7fd60d863415
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Implementation of the asparaginase activity assessment technique for clinical use: experience of a Brazilian Center

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