New insights into the standard method of assessing bacterial filtration efficiency of medical face masks

Abstract Based on the current knowledge of severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) transmission, wearing a mask has been recommended during the COVID-19 pandemic. Bacterial filtration efficiency (BFE) measurements enable designing and regulating medical masks to prevent bioaeros...

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Autores principales:	Jérémie Pourchez, Aurélien Peyron, Yoann Montigaud, Coralie Laurent, Estelle Audoux, Lara Leclerc, Paul O. Verhoeven
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/3f05c68b75b043a6892306c5e798ee92
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spelling	oai:doaj.org-article:3f05c68b75b043a6892306c5e798ee922021-12-02T17:05:00ZNew insights into the standard method of assessing bacterial filtration efficiency of medical face masks10.1038/s41598-021-85327-x2045-2322https://doaj.org/article/3f05c68b75b043a6892306c5e798ee922021-03-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-85327-xhttps://doaj.org/toc/2045-2322Abstract Based on the current knowledge of severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) transmission, wearing a mask has been recommended during the COVID-19 pandemic. Bacterial filtration efficiency (BFE) measurements enable designing and regulating medical masks to prevent bioaerosol dissemination; however, despite the simplicity of these measurements, several scientific questions remain unanswered regarding BFE tests. Here, we investigated (1) the impact of substituting 100-mm Petri dishes with 90-mm disposable Petri dishes, (2) the impact of colony-counting methods on the bioaerosol aerodynamic size, and (3) the impact of colony-counting methods on the total viable particle counts. We demonstrated that disposable 90-mm Petri dishes can be used to replace the 100-mm dishes. We also showed that an automatic high-resolution colony counter can be used to directly count viable particles on collection substrates and to measure the bioaerosol size parameters. Our results enable possible modernization of the outdated testing methods recommended in the US and European standards for BFE measurements. Specifically, use of a modernized colony counter should be clearly regulated and permitted to avoid the counting of positive holes. The median aerodynamic diameter appears to be the most relevant parameter for characterizing bioaerosol size.Jérémie PourchezAurélien PeyronYoann MontigaudCoralie LaurentEstelle AudouxLara LeclercPaul O. VerhoevenNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-11 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Jérémie Pourchez Aurélien Peyron Yoann Montigaud Coralie Laurent Estelle Audoux Lara Leclerc Paul O. Verhoeven New insights into the standard method of assessing bacterial filtration efficiency of medical face masks
description	Abstract Based on the current knowledge of severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) transmission, wearing a mask has been recommended during the COVID-19 pandemic. Bacterial filtration efficiency (BFE) measurements enable designing and regulating medical masks to prevent bioaerosol dissemination; however, despite the simplicity of these measurements, several scientific questions remain unanswered regarding BFE tests. Here, we investigated (1) the impact of substituting 100-mm Petri dishes with 90-mm disposable Petri dishes, (2) the impact of colony-counting methods on the bioaerosol aerodynamic size, and (3) the impact of colony-counting methods on the total viable particle counts. We demonstrated that disposable 90-mm Petri dishes can be used to replace the 100-mm dishes. We also showed that an automatic high-resolution colony counter can be used to directly count viable particles on collection substrates and to measure the bioaerosol size parameters. Our results enable possible modernization of the outdated testing methods recommended in the US and European standards for BFE measurements. Specifically, use of a modernized colony counter should be clearly regulated and permitted to avoid the counting of positive holes. The median aerodynamic diameter appears to be the most relevant parameter for characterizing bioaerosol size.
format	article
author	Jérémie Pourchez Aurélien Peyron Yoann Montigaud Coralie Laurent Estelle Audoux Lara Leclerc Paul O. Verhoeven
author_facet	Jérémie Pourchez Aurélien Peyron Yoann Montigaud Coralie Laurent Estelle Audoux Lara Leclerc Paul O. Verhoeven
author_sort	Jérémie Pourchez
title	New insights into the standard method of assessing bacterial filtration efficiency of medical face masks
title_short	New insights into the standard method of assessing bacterial filtration efficiency of medical face masks
title_full	New insights into the standard method of assessing bacterial filtration efficiency of medical face masks
title_fullStr	New insights into the standard method of assessing bacterial filtration efficiency of medical face masks
title_full_unstemmed	New insights into the standard method of assessing bacterial filtration efficiency of medical face masks
title_sort	new insights into the standard method of assessing bacterial filtration efficiency of medical face masks
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/3f05c68b75b043a6892306c5e798ee92
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New insights into the standard method of assessing bacterial filtration efficiency of medical face masks

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