An Aptamer-Array-Based Sample-to-Answer Biosensor for Ochratoxin A Detection via Fluorescence Resonance Energy Transfer

Food toxins are a hidden threat that can cause cancer and tremendously impact human health. Therefore, the detection of food toxins in a timely manner with high sensitivity is of paramount importance for public health and food safety. However, the current detection methods are relatively time-consum...

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Autores principales:	Yongning Li, Zhenfei Peng, Yaxi Li, Min Xiao, Gongjun Tan, Wenlian Wang, Yu Wang, Min Fang, Shu Zhang, Chenling Tang, Bowen Yang, Tianfu Wu
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	aptamer ochratoxin A FRET on-chip assay Biochemistry QD415-436
Acceso en línea:	https://doaj.org/article/3f079f4419cf411c875340c1b0c2e025
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spelling	oai:doaj.org-article:3f079f4419cf411c875340c1b0c2e0252021-11-25T17:13:34ZAn Aptamer-Array-Based Sample-to-Answer Biosensor for Ochratoxin A Detection via Fluorescence Resonance Energy Transfer10.3390/chemosensors91103092227-9040https://doaj.org/article/3f079f4419cf411c875340c1b0c2e0252021-10-01T00:00:00Zhttps://www.mdpi.com/2227-9040/9/11/309https://doaj.org/toc/2227-9040Food toxins are a hidden threat that can cause cancer and tremendously impact human health. Therefore, the detection of food toxins in a timely manner with high sensitivity is of paramount importance for public health and food safety. However, the current detection methods are relatively time-consuming and not practical for field tests. In the present work, we developed a novel aptamer-chip-based sample-to-answer biosensor (ACSB) for ochratoxin A (OTA) detection via fluorescence resonance energy transfer (FRET). In this system, a cyanine 3 (Cy3)-labeled OTA-specific biotinylated aptamer was immobilized on an epoxy-coated chip via streptavidin-biotin binding. A complementary DNA strand to OTA aptamer at the 3′-end was labeled with a black hole quencher 2 (BHQ2) to quench Cy3 fluorescence when in proximity. In the presence of OTA, the Cy3-labeled OTA aptamer bound specifically to OTA and led to the physical separation of Cy3 and BHQ2, which resulted in an increase of fluorescence signal. The limit of detection (LOD) of this ACSB for OTA was 0.005 ng/mL with a linearity range of 0.01–10 ng/mL. The cross-reactivity of ACSB against other mycotoxins, ochratoxin B (OTB), aflatoxin B1 (AFB1), zearalenone (ZEA), or deoxynilvalenol (DON), was less than 0.01%. In addition, this system could accurately detect OTA in rice samples spiked with OTA, and the mean recovery rate of the spiked-in OTA reached 91%, with a coefficient of variation (CV) of 8.57–9.89%. Collectively, the ACSB may represent a rapid, accurate, and easy-to-use platform for OTA detection with high sensitivity and specificity.Yongning LiZhenfei PengYaxi LiMin XiaoGongjun TanWenlian WangYu WangMin FangShu ZhangChenling TangBowen YangTianfu WuMDPI AGarticleaptamerochratoxin AFRETon-chip assayBiochemistryQD415-436ENChemosensors, Vol 9, Iss 309, p 309 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	aptamer ochratoxin A FRET on-chip assay Biochemistry QD415-436
spellingShingle	aptamer ochratoxin A FRET on-chip assay Biochemistry QD415-436 Yongning Li Zhenfei Peng Yaxi Li Min Xiao Gongjun Tan Wenlian Wang Yu Wang Min Fang Shu Zhang Chenling Tang Bowen Yang Tianfu Wu An Aptamer-Array-Based Sample-to-Answer Biosensor for Ochratoxin A Detection via Fluorescence Resonance Energy Transfer
description	Food toxins are a hidden threat that can cause cancer and tremendously impact human health. Therefore, the detection of food toxins in a timely manner with high sensitivity is of paramount importance for public health and food safety. However, the current detection methods are relatively time-consuming and not practical for field tests. In the present work, we developed a novel aptamer-chip-based sample-to-answer biosensor (ACSB) for ochratoxin A (OTA) detection via fluorescence resonance energy transfer (FRET). In this system, a cyanine 3 (Cy3)-labeled OTA-specific biotinylated aptamer was immobilized on an epoxy-coated chip via streptavidin-biotin binding. A complementary DNA strand to OTA aptamer at the 3′-end was labeled with a black hole quencher 2 (BHQ2) to quench Cy3 fluorescence when in proximity. In the presence of OTA, the Cy3-labeled OTA aptamer bound specifically to OTA and led to the physical separation of Cy3 and BHQ2, which resulted in an increase of fluorescence signal. The limit of detection (LOD) of this ACSB for OTA was 0.005 ng/mL with a linearity range of 0.01–10 ng/mL. The cross-reactivity of ACSB against other mycotoxins, ochratoxin B (OTB), aflatoxin B1 (AFB1), zearalenone (ZEA), or deoxynilvalenol (DON), was less than 0.01%. In addition, this system could accurately detect OTA in rice samples spiked with OTA, and the mean recovery rate of the spiked-in OTA reached 91%, with a coefficient of variation (CV) of 8.57–9.89%. Collectively, the ACSB may represent a rapid, accurate, and easy-to-use platform for OTA detection with high sensitivity and specificity.
format	article
author	Yongning Li Zhenfei Peng Yaxi Li Min Xiao Gongjun Tan Wenlian Wang Yu Wang Min Fang Shu Zhang Chenling Tang Bowen Yang Tianfu Wu
author_facet	Yongning Li Zhenfei Peng Yaxi Li Min Xiao Gongjun Tan Wenlian Wang Yu Wang Min Fang Shu Zhang Chenling Tang Bowen Yang Tianfu Wu
author_sort	Yongning Li
title	An Aptamer-Array-Based Sample-to-Answer Biosensor for Ochratoxin A Detection via Fluorescence Resonance Energy Transfer
title_short	An Aptamer-Array-Based Sample-to-Answer Biosensor for Ochratoxin A Detection via Fluorescence Resonance Energy Transfer
title_full	An Aptamer-Array-Based Sample-to-Answer Biosensor for Ochratoxin A Detection via Fluorescence Resonance Energy Transfer
title_fullStr	An Aptamer-Array-Based Sample-to-Answer Biosensor for Ochratoxin A Detection via Fluorescence Resonance Energy Transfer
title_full_unstemmed	An Aptamer-Array-Based Sample-to-Answer Biosensor for Ochratoxin A Detection via Fluorescence Resonance Energy Transfer
title_sort	aptamer-array-based sample-to-answer biosensor for ochratoxin a detection via fluorescence resonance energy transfer
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/3f079f4419cf411c875340c1b0c2e025
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An Aptamer-Array-Based Sample-to-Answer Biosensor for Ochratoxin A Detection via Fluorescence Resonance Energy Transfer

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