Sprouty3, but Not Sprouty1, Expression Is Beneficial for the Malignant Potential of Osteosarcoma Cells

Sprouty proteins are widely accepted modulators of receptor tyrosine kinase-associated pathways and fulfill diversified roles in cancerogenesis dependent on the originating cells. In this study we detected a high expression of Sprouty3 in osteosarcoma-derived cells and addressed the question of whet...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Anna Zita Mehira Kamptner, Christoph-Erik Mayer, Hedwig Sutterlüty
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	Sprouty3 osteosarcoma Spry3 Spry1 tumor promoter MAPK pathway Biology (General) QH301-705.5 Chemistry QD1-999
Acceso en línea:	https://doaj.org/article/3f094acb9c254e2a8050ab0133650483
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:3f094acb9c254e2a8050ab0133650483
record_format	dspace
spelling	oai:doaj.org-article:3f094acb9c254e2a8050ab01336504832021-11-11T17:21:59ZSprouty3, but Not Sprouty1, Expression Is Beneficial for the Malignant Potential of Osteosarcoma Cells10.3390/ijms2221119441422-00671661-6596https://doaj.org/article/3f094acb9c254e2a8050ab01336504832021-11-01T00:00:00Zhttps://www.mdpi.com/1422-0067/22/21/11944https://doaj.org/toc/1661-6596https://doaj.org/toc/1422-0067Sprouty proteins are widely accepted modulators of receptor tyrosine kinase-associated pathways and fulfill diversified roles in cancerogenesis dependent on the originating cells. In this study we detected a high expression of Sprouty3 in osteosarcoma-derived cells and addressed the question of whether Sprouty3 and Sprouty1 influence the malignant phenotype of this bone tumor entity. By using adenoviruses, the Sprouty proteins were expressed in two different cell lines and their influence on cellular behavior was assessed. Growth curve analyses and Scratch assays revealed that Sprouty3 accelerates cell proliferation and migration. Additionally, more colonies were grown in Soft agar if the cells express Sprouty3. In parallel, Sprouty1 had no significant effect on the measured endpoints of the study in osteosarcoma-derived cells. The promotion of the tumorigenic capacities in the presence of Sprouty3 coincided with an increased activation of signaling as measured by evaluating the phosphorylation of extracellular signal-regulated kinases (ERKs). Ectopic expression of a mutated Sprouty3 protein, in which the tyrosine necessary for its activation was substituted, resulted in inhibited migration of the treated cells. Our findings identify Sprouty3 as a candidate for a tumor promoter in osteosarcoma.Anna Zita Mehira KamptnerChristoph-Erik MayerHedwig SutterlütyMDPI AGarticleSprouty3osteosarcomaSpry3Spry1tumor promoterMAPK pathwayBiology (General)QH301-705.5ChemistryQD1-999ENInternational Journal of Molecular Sciences, Vol 22, Iss 11944, p 11944 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Sprouty3 osteosarcoma Spry3 Spry1 tumor promoter MAPK pathway Biology (General) QH301-705.5 Chemistry QD1-999
spellingShingle	Sprouty3 osteosarcoma Spry3 Spry1 tumor promoter MAPK pathway Biology (General) QH301-705.5 Chemistry QD1-999 Anna Zita Mehira Kamptner Christoph-Erik Mayer Hedwig Sutterlüty Sprouty3, but Not Sprouty1, Expression Is Beneficial for the Malignant Potential of Osteosarcoma Cells
description	Sprouty proteins are widely accepted modulators of receptor tyrosine kinase-associated pathways and fulfill diversified roles in cancerogenesis dependent on the originating cells. In this study we detected a high expression of Sprouty3 in osteosarcoma-derived cells and addressed the question of whether Sprouty3 and Sprouty1 influence the malignant phenotype of this bone tumor entity. By using adenoviruses, the Sprouty proteins were expressed in two different cell lines and their influence on cellular behavior was assessed. Growth curve analyses and Scratch assays revealed that Sprouty3 accelerates cell proliferation and migration. Additionally, more colonies were grown in Soft agar if the cells express Sprouty3. In parallel, Sprouty1 had no significant effect on the measured endpoints of the study in osteosarcoma-derived cells. The promotion of the tumorigenic capacities in the presence of Sprouty3 coincided with an increased activation of signaling as measured by evaluating the phosphorylation of extracellular signal-regulated kinases (ERKs). Ectopic expression of a mutated Sprouty3 protein, in which the tyrosine necessary for its activation was substituted, resulted in inhibited migration of the treated cells. Our findings identify Sprouty3 as a candidate for a tumor promoter in osteosarcoma.
format	article
author	Anna Zita Mehira Kamptner Christoph-Erik Mayer Hedwig Sutterlüty
author_facet	Anna Zita Mehira Kamptner Christoph-Erik Mayer Hedwig Sutterlüty
author_sort	Anna Zita Mehira Kamptner
title	Sprouty3, but Not Sprouty1, Expression Is Beneficial for the Malignant Potential of Osteosarcoma Cells
title_short	Sprouty3, but Not Sprouty1, Expression Is Beneficial for the Malignant Potential of Osteosarcoma Cells
title_full	Sprouty3, but Not Sprouty1, Expression Is Beneficial for the Malignant Potential of Osteosarcoma Cells
title_fullStr	Sprouty3, but Not Sprouty1, Expression Is Beneficial for the Malignant Potential of Osteosarcoma Cells
title_full_unstemmed	Sprouty3, but Not Sprouty1, Expression Is Beneficial for the Malignant Potential of Osteosarcoma Cells
title_sort	sprouty3, but not sprouty1, expression is beneficial for the malignant potential of osteosarcoma cells
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/3f094acb9c254e2a8050ab0133650483
work_keys_str_mv	AT annazitamehirakamptner sprouty3butnotsprouty1expressionisbeneficialforthemalignantpotentialofosteosarcomacells AT christopherikmayer sprouty3butnotsprouty1expressionisbeneficialforthemalignantpotentialofosteosarcomacells AT hedwigsutterluty sprouty3butnotsprouty1expressionisbeneficialforthemalignantpotentialofosteosarcomacells
_version_	1718432112411934720

Sprouty3, but Not Sprouty1, Expression Is Beneficial for the Malignant Potential of Osteosarcoma Cells

Ejemplares similares