The von Willebrand factor stamps plasmatic extracellular vesicles from glioblastoma patients
Abstract Glioblastoma is a devastating tumor of the central nervous system characterized by a poor survival and an extremely dark prognosis, making its diagnosis, treatment and monitoring highly challenging. Numerous studies have highlighted extracellular vesicles (EVs) as key players of tumor growt...
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Nature Portfolio
2021
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oai:doaj.org-article:3f0bf5fe470e45c09306ee760b7649cb2021-11-28T12:21:41ZThe von Willebrand factor stamps plasmatic extracellular vesicles from glioblastoma patients10.1038/s41598-021-02254-72045-2322https://doaj.org/article/3f0bf5fe470e45c09306ee760b7649cb2021-11-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-02254-7https://doaj.org/toc/2045-2322Abstract Glioblastoma is a devastating tumor of the central nervous system characterized by a poor survival and an extremely dark prognosis, making its diagnosis, treatment and monitoring highly challenging. Numerous studies have highlighted extracellular vesicles (EVs) as key players of tumor growth, invasiveness and resistance, as they carry and disseminate oncogenic material in the local tumor microenvironment and at distance. However, whether their quality and quantity reflect individual health status and changes in homeostasis is still not fully elucidated. Here, we separated EVs from plasma collected at different time points alongside with the clinical management of GBM patients. Our findings confirm that plasmatic EVs could be separated and characterized with standardized protocols, thereby ensuring the reliability of measuring vesiclemia, i.e. extracellular vesicle concentration in plasma. This unveils that vesiclemia is a dynamic parameter, which could be reflecting tumor burden and/or response to treatments. Further label-free liquid chromatography tandem mass spectrometry unmasks the von Willebrand Factor (VWF) as a selective protein hallmark for GBM-patient EVs. Our data thus support the notion that EVs from GBM patients showed differential protein cargos that can be further surveyed in circulating EVs, together with vesiclemia.Quentin SabbaghGwennan André-GrégoireCarolina Alves-NicolauAurélien DupontNicolas BidèreEmmanuel JouglarLaëtitia GuévelJean-Sébastien FrénelJulie GavardNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-11 (2021) |
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Medicine R Science Q Quentin Sabbagh Gwennan André-Grégoire Carolina Alves-Nicolau Aurélien Dupont Nicolas Bidère Emmanuel Jouglar Laëtitia Guével Jean-Sébastien Frénel Julie Gavard The von Willebrand factor stamps plasmatic extracellular vesicles from glioblastoma patients |
| description |
Abstract Glioblastoma is a devastating tumor of the central nervous system characterized by a poor survival and an extremely dark prognosis, making its diagnosis, treatment and monitoring highly challenging. Numerous studies have highlighted extracellular vesicles (EVs) as key players of tumor growth, invasiveness and resistance, as they carry and disseminate oncogenic material in the local tumor microenvironment and at distance. However, whether their quality and quantity reflect individual health status and changes in homeostasis is still not fully elucidated. Here, we separated EVs from plasma collected at different time points alongside with the clinical management of GBM patients. Our findings confirm that plasmatic EVs could be separated and characterized with standardized protocols, thereby ensuring the reliability of measuring vesiclemia, i.e. extracellular vesicle concentration in plasma. This unveils that vesiclemia is a dynamic parameter, which could be reflecting tumor burden and/or response to treatments. Further label-free liquid chromatography tandem mass spectrometry unmasks the von Willebrand Factor (VWF) as a selective protein hallmark for GBM-patient EVs. Our data thus support the notion that EVs from GBM patients showed differential protein cargos that can be further surveyed in circulating EVs, together with vesiclemia. |
| format |
article |
| author |
Quentin Sabbagh Gwennan André-Grégoire Carolina Alves-Nicolau Aurélien Dupont Nicolas Bidère Emmanuel Jouglar Laëtitia Guével Jean-Sébastien Frénel Julie Gavard |
| author_facet |
Quentin Sabbagh Gwennan André-Grégoire Carolina Alves-Nicolau Aurélien Dupont Nicolas Bidère Emmanuel Jouglar Laëtitia Guével Jean-Sébastien Frénel Julie Gavard |
| author_sort |
Quentin Sabbagh |
| title |
The von Willebrand factor stamps plasmatic extracellular vesicles from glioblastoma patients |
| title_short |
The von Willebrand factor stamps plasmatic extracellular vesicles from glioblastoma patients |
| title_full |
The von Willebrand factor stamps plasmatic extracellular vesicles from glioblastoma patients |
| title_fullStr |
The von Willebrand factor stamps plasmatic extracellular vesicles from glioblastoma patients |
| title_full_unstemmed |
The von Willebrand factor stamps plasmatic extracellular vesicles from glioblastoma patients |
| title_sort |
von willebrand factor stamps plasmatic extracellular vesicles from glioblastoma patients |
| publisher |
Nature Portfolio |
| publishDate |
2021 |
| url |
https://doaj.org/article/3f0bf5fe470e45c09306ee760b7649cb |
| work_keys_str_mv |
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