AmotL2 integrates polarity and junctional cues to modulate cell shape

Abstract The assembly of individual epithelial or endothelial cells into a tight cellular sheet requires stringent control of cell packing and organization. These processes are dependent on the establishment and further integration of cellular junctions, the cytoskeleton and the formation of apical-...

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Autores principales:	Sara Hultin, Aravindh Subramani, Sebastian Hildebrand, Yujuan Zheng, Arindam Majumdar, Lars Holmgren
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/3f0edef230f64693b0028e366debdf52
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spelling	oai:doaj.org-article:3f0edef230f64693b0028e366debdf522021-12-02T12:31:48ZAmotL2 integrates polarity and junctional cues to modulate cell shape10.1038/s41598-017-07968-12045-2322https://doaj.org/article/3f0edef230f64693b0028e366debdf522017-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-07968-1https://doaj.org/toc/2045-2322Abstract The assembly of individual epithelial or endothelial cells into a tight cellular sheet requires stringent control of cell packing and organization. These processes are dependent on the establishment and further integration of cellular junctions, the cytoskeleton and the formation of apical-basal polarity. However, little is known how these subcellular events are coordinated. The (Angiomotin) Amot protein family consists of scaffold proteins that interact with junctional cadherins, polarity proteins and the cytoskeleton. In this report, we have studied how these protein complexes integrate to control cellular shapes consistent with organ function. Using gene-inactivating studies in zebrafish and cell culture systems in vitro, we show that Par3 to be essential for localization of AmotL2 to cellular junctions to associate with VE/E-cadherin and subsequently the organization of radial actin filaments. Our data provide mechanistic insight in how critical processes such as aortic lumen expansion as well as epithelial packing into hexagonal shapes are controlled.Sara HultinAravindh SubramaniSebastian HildebrandYujuan ZhengArindam MajumdarLars HolmgrenNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-10 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Sara Hultin Aravindh Subramani Sebastian Hildebrand Yujuan Zheng Arindam Majumdar Lars Holmgren AmotL2 integrates polarity and junctional cues to modulate cell shape
description	Abstract The assembly of individual epithelial or endothelial cells into a tight cellular sheet requires stringent control of cell packing and organization. These processes are dependent on the establishment and further integration of cellular junctions, the cytoskeleton and the formation of apical-basal polarity. However, little is known how these subcellular events are coordinated. The (Angiomotin) Amot protein family consists of scaffold proteins that interact with junctional cadherins, polarity proteins and the cytoskeleton. In this report, we have studied how these protein complexes integrate to control cellular shapes consistent with organ function. Using gene-inactivating studies in zebrafish and cell culture systems in vitro, we show that Par3 to be essential for localization of AmotL2 to cellular junctions to associate with VE/E-cadherin and subsequently the organization of radial actin filaments. Our data provide mechanistic insight in how critical processes such as aortic lumen expansion as well as epithelial packing into hexagonal shapes are controlled.
format	article
author	Sara Hultin Aravindh Subramani Sebastian Hildebrand Yujuan Zheng Arindam Majumdar Lars Holmgren
author_facet	Sara Hultin Aravindh Subramani Sebastian Hildebrand Yujuan Zheng Arindam Majumdar Lars Holmgren
author_sort	Sara Hultin
title	AmotL2 integrates polarity and junctional cues to modulate cell shape
title_short	AmotL2 integrates polarity and junctional cues to modulate cell shape
title_full	AmotL2 integrates polarity and junctional cues to modulate cell shape
title_fullStr	AmotL2 integrates polarity and junctional cues to modulate cell shape
title_full_unstemmed	AmotL2 integrates polarity and junctional cues to modulate cell shape
title_sort	amotl2 integrates polarity and junctional cues to modulate cell shape
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/3f0edef230f64693b0028e366debdf52
work_keys_str_mv	AT sarahultin amotl2integratespolarityandjunctionalcuestomodulatecellshape AT aravindhsubramani amotl2integratespolarityandjunctionalcuestomodulatecellshape AT sebastianhildebrand amotl2integratespolarityandjunctionalcuestomodulatecellshape AT yujuanzheng amotl2integratespolarityandjunctionalcuestomodulatecellshape AT arindammajumdar amotl2integratespolarityandjunctionalcuestomodulatecellshape AT larsholmgren amotl2integratespolarityandjunctionalcuestomodulatecellshape
_version_	1718394272378519552

AmotL2 integrates polarity and junctional cues to modulate cell shape

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