Single cell transcriptomics reveals the heterogeneity of the human cornea to identify novel markers of the limbus and stroma

Abstract The cornea is the clear window that lets light into the eye. It is composed of five layers: epithelium, Bowman’s layer, stroma, Descemet’s membrane and endothelium. The maintenance of its structure and transparency are determined by the functions of the different cell types populating each...

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Autores principales:	Pere Català, Nathalie Groen, Jasmin A. Dehnen, Eduardo Soares, Arianne J. H. van Velthoven, Rudy M. M. A. Nuijts, Mor M. Dickman, Vanessa L. S. LaPointe
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/3f12ca026b0c49c7999b3af4c6267818
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spelling	oai:doaj.org-article:3f12ca026b0c49c7999b3af4c62678182021-11-08T10:48:05ZSingle cell transcriptomics reveals the heterogeneity of the human cornea to identify novel markers of the limbus and stroma10.1038/s41598-021-01015-w2045-2322https://doaj.org/article/3f12ca026b0c49c7999b3af4c62678182021-11-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-01015-whttps://doaj.org/toc/2045-2322Abstract The cornea is the clear window that lets light into the eye. It is composed of five layers: epithelium, Bowman’s layer, stroma, Descemet’s membrane and endothelium. The maintenance of its structure and transparency are determined by the functions of the different cell types populating each layer. Attempts to regenerate corneal tissue and understand disease conditions requires knowledge of how cell profiles vary across this heterogeneous tissue. We performed a single cell transcriptomic profiling of 19,472 cells isolated from eight healthy donor corneas. Our analysis delineates the heterogeneity of the corneal layers by identifying cell populations and revealing cell states that contribute in preserving corneal homeostasis. We identified expression of CAV1, HOMER3 and CPVL in the corneal epithelial limbal stem cell niche, CKS2, STMN1 and UBE2C were exclusively expressed in highly proliferative transit amplifying cells, CXCL14 was expressed exclusively in the suprabasal/superficial limbus, and NNMT was exclusively expressed by stromal keratocytes. Overall, this research provides a basis to improve current primary cell expansion protocols, for future profiling of corneal disease states, to help guide pluripotent stem cells into different corneal lineages, and to understand how engineered substrates affect corneal cells to improve regenerative therapies.Pere CatalàNathalie GroenJasmin A. DehnenEduardo SoaresArianne J. H. van VelthovenRudy M. M. A. NuijtsMor M. DickmanVanessa L. S. LaPointeNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-13 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Pere Català Nathalie Groen Jasmin A. Dehnen Eduardo Soares Arianne J. H. van Velthoven Rudy M. M. A. Nuijts Mor M. Dickman Vanessa L. S. LaPointe Single cell transcriptomics reveals the heterogeneity of the human cornea to identify novel markers of the limbus and stroma
description	Abstract The cornea is the clear window that lets light into the eye. It is composed of five layers: epithelium, Bowman’s layer, stroma, Descemet’s membrane and endothelium. The maintenance of its structure and transparency are determined by the functions of the different cell types populating each layer. Attempts to regenerate corneal tissue and understand disease conditions requires knowledge of how cell profiles vary across this heterogeneous tissue. We performed a single cell transcriptomic profiling of 19,472 cells isolated from eight healthy donor corneas. Our analysis delineates the heterogeneity of the corneal layers by identifying cell populations and revealing cell states that contribute in preserving corneal homeostasis. We identified expression of CAV1, HOMER3 and CPVL in the corneal epithelial limbal stem cell niche, CKS2, STMN1 and UBE2C were exclusively expressed in highly proliferative transit amplifying cells, CXCL14 was expressed exclusively in the suprabasal/superficial limbus, and NNMT was exclusively expressed by stromal keratocytes. Overall, this research provides a basis to improve current primary cell expansion protocols, for future profiling of corneal disease states, to help guide pluripotent stem cells into different corneal lineages, and to understand how engineered substrates affect corneal cells to improve regenerative therapies.
format	article
author	Pere Català Nathalie Groen Jasmin A. Dehnen Eduardo Soares Arianne J. H. van Velthoven Rudy M. M. A. Nuijts Mor M. Dickman Vanessa L. S. LaPointe
author_facet	Pere Català Nathalie Groen Jasmin A. Dehnen Eduardo Soares Arianne J. H. van Velthoven Rudy M. M. A. Nuijts Mor M. Dickman Vanessa L. S. LaPointe
author_sort	Pere Català
title	Single cell transcriptomics reveals the heterogeneity of the human cornea to identify novel markers of the limbus and stroma
title_short	Single cell transcriptomics reveals the heterogeneity of the human cornea to identify novel markers of the limbus and stroma
title_full	Single cell transcriptomics reveals the heterogeneity of the human cornea to identify novel markers of the limbus and stroma
title_fullStr	Single cell transcriptomics reveals the heterogeneity of the human cornea to identify novel markers of the limbus and stroma
title_full_unstemmed	Single cell transcriptomics reveals the heterogeneity of the human cornea to identify novel markers of the limbus and stroma
title_sort	single cell transcriptomics reveals the heterogeneity of the human cornea to identify novel markers of the limbus and stroma
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/3f12ca026b0c49c7999b3af4c6267818
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Single cell transcriptomics reveals the heterogeneity of the human cornea to identify novel markers of the limbus and stroma

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