EXPERIMENTAL STUDY OF THE EFFECTIVENESS OF THE JOINT THERAPEUTIC ACTION OF LACTOGLOBULIN AND SALLMONELLA BACTERIOPHAGE

The wide spread of drug-resistant forms of the pathogens, complicating prevention and treatment of acute intestinal infections, determines the feasibility of using bacteriophages and immune lactoglobulins for antibacterial therapy of these diseases. The aim of this study was to investigate the antib...

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Autores principales:	N. V. Aleksanina, O. V. Moiseyeva
Formato:	article
Lenguaje:	RU
Publicado:	Sankt-Peterburg : NIIÈM imeni Pastera 2017
Materias:	salmonella infection lactoglobulin bacteriophage animals experiment Infectious and parasitic diseases RC109-216
Acceso en línea:	https://doaj.org/article/3f155f07bed24022996ff769f5a7f01d
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spelling	oai:doaj.org-article:3f155f07bed24022996ff769f5a7f01d2021-11-22T07:09:48ZEXPERIMENTAL STUDY OF THE EFFECTIVENESS OF THE JOINT THERAPEUTIC ACTION OF LACTOGLOBULIN AND SALLMONELLA BACTERIOPHAGE2220-76192313-739810.15789/2220-7619-2017-3-297-302https://doaj.org/article/3f155f07bed24022996ff769f5a7f01d2017-09-01T00:00:00Zhttps://www.iimmun.ru/iimm/article/view/563https://doaj.org/toc/2220-7619https://doaj.org/toc/2313-7398The wide spread of drug-resistant forms of the pathogens, complicating prevention and treatment of acute intestinal infections, determines the feasibility of using bacteriophages and immune lactoglobulins for antibacterial therapy of these diseases. The aim of this study was to investigate the antibacterial efficacy of the joint use of immune lactoglobulin and Salmonella bacteriophage in the treatment of experimental salmonellosis in mice. The experimental Salmonella infection was reproduced by means of a single intragastric administration of 2LD50 of daily agar culture of S.typhimurium. As a result, the development of Salmonella infection lasting up to 14 days was noted. The animals were divided into four groups according to the pattern of the treatment. The following preparations were used for the treatment of animals: «Lactoglobulin against opportunistic bacteria and Salmonella» dry cow, «Lactoglobulin» liquid, bacteriophage of Salmonella ABCDE groups, liquid. The preparations were daily administered to mice orally after the infection for 7 days. In the study of the joint therapeutic effect of the lactoglobulin and bacteriophage the special attention was paid to the terms of elimination of the pathogen from animal organs. Analysis of the data showed that in all four groups a positive dynamics of excretion of the pathogen of mice was revealed. In groups of animals that were treated with both drugs, the elimination of Salmonella out of organs occurred 1.5 times faster than in the control groups of mice. At the same time in the experimental groups on the third day of treatment with preparations the statistically significant decrease in the number of S.typhimurium in the contents of the large intestine of animal was revealed. The study found that immune lactoglobulin used simultaneously with Salmonella phage for 7 days reduced the severity and duration of the disease course and blocked the further development of the infectious process, promoted the efficient and rapid cleansing of animals organism of the pathogen, increased the survival of mice. The obtained data suggest the applicability of joint use of immune lactoglobulin preparations and bacteriophages in the treatment of acute intestinal infections caused by antibiotic-resistant strains of Salmonella.N. V. AleksaninaO. V. MoiseyevaSankt-Peterburg : NIIÈM imeni Pasteraarticlesalmonella infectionlactoglobulinbacteriophageanimalsexperimentInfectious and parasitic diseasesRC109-216RUInfekciâ i Immunitet, Vol 7, Iss 3, Pp 297-302 (2017)
institution	DOAJ
collection	DOAJ
language	RU
topic	salmonella infection lactoglobulin bacteriophage animals experiment Infectious and parasitic diseases RC109-216
spellingShingle	salmonella infection lactoglobulin bacteriophage animals experiment Infectious and parasitic diseases RC109-216 N. V. Aleksanina O. V. Moiseyeva EXPERIMENTAL STUDY OF THE EFFECTIVENESS OF THE JOINT THERAPEUTIC ACTION OF LACTOGLOBULIN AND SALLMONELLA BACTERIOPHAGE
description	The wide spread of drug-resistant forms of the pathogens, complicating prevention and treatment of acute intestinal infections, determines the feasibility of using bacteriophages and immune lactoglobulins for antibacterial therapy of these diseases. The aim of this study was to investigate the antibacterial efficacy of the joint use of immune lactoglobulin and Salmonella bacteriophage in the treatment of experimental salmonellosis in mice. The experimental Salmonella infection was reproduced by means of a single intragastric administration of 2LD50 of daily agar culture of S.typhimurium. As a result, the development of Salmonella infection lasting up to 14 days was noted. The animals were divided into four groups according to the pattern of the treatment. The following preparations were used for the treatment of animals: «Lactoglobulin against opportunistic bacteria and Salmonella» dry cow, «Lactoglobulin» liquid, bacteriophage of Salmonella ABCDE groups, liquid. The preparations were daily administered to mice orally after the infection for 7 days. In the study of the joint therapeutic effect of the lactoglobulin and bacteriophage the special attention was paid to the terms of elimination of the pathogen from animal organs. Analysis of the data showed that in all four groups a positive dynamics of excretion of the pathogen of mice was revealed. In groups of animals that were treated with both drugs, the elimination of Salmonella out of organs occurred 1.5 times faster than in the control groups of mice. At the same time in the experimental groups on the third day of treatment with preparations the statistically significant decrease in the number of S.typhimurium in the contents of the large intestine of animal was revealed. The study found that immune lactoglobulin used simultaneously with Salmonella phage for 7 days reduced the severity and duration of the disease course and blocked the further development of the infectious process, promoted the efficient and rapid cleansing of animals organism of the pathogen, increased the survival of mice. The obtained data suggest the applicability of joint use of immune lactoglobulin preparations and bacteriophages in the treatment of acute intestinal infections caused by antibiotic-resistant strains of Salmonella.
format	article
author	N. V. Aleksanina O. V. Moiseyeva
author_facet	N. V. Aleksanina O. V. Moiseyeva
author_sort	N. V. Aleksanina
title	EXPERIMENTAL STUDY OF THE EFFECTIVENESS OF THE JOINT THERAPEUTIC ACTION OF LACTOGLOBULIN AND SALLMONELLA BACTERIOPHAGE
title_short	EXPERIMENTAL STUDY OF THE EFFECTIVENESS OF THE JOINT THERAPEUTIC ACTION OF LACTOGLOBULIN AND SALLMONELLA BACTERIOPHAGE
title_full	EXPERIMENTAL STUDY OF THE EFFECTIVENESS OF THE JOINT THERAPEUTIC ACTION OF LACTOGLOBULIN AND SALLMONELLA BACTERIOPHAGE
title_fullStr	EXPERIMENTAL STUDY OF THE EFFECTIVENESS OF THE JOINT THERAPEUTIC ACTION OF LACTOGLOBULIN AND SALLMONELLA BACTERIOPHAGE
title_full_unstemmed	EXPERIMENTAL STUDY OF THE EFFECTIVENESS OF THE JOINT THERAPEUTIC ACTION OF LACTOGLOBULIN AND SALLMONELLA BACTERIOPHAGE
title_sort	experimental study of the effectiveness of the joint therapeutic action of lactoglobulin and sallmonella bacteriophage
publisher	Sankt-Peterburg : NIIÈM imeni Pastera
publishDate	2017
url	https://doaj.org/article/3f155f07bed24022996ff769f5a7f01d
work_keys_str_mv	AT nvaleksanina experimentalstudyoftheeffectivenessofthejointtherapeuticactionoflactoglobulinandsallmonellabacteriophage AT ovmoiseyeva experimentalstudyoftheeffectivenessofthejointtherapeuticactionoflactoglobulinandsallmonellabacteriophage
_version_	1718418034784206848

EXPERIMENTAL STUDY OF THE EFFECTIVENESS OF THE JOINT THERAPEUTIC ACTION OF LACTOGLOBULIN AND SALLMONELLA BACTERIOPHAGE

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