CmRCC1 Gene From Pumpkin Confers Cold Tolerance in Tobacco by Modulating Root Architecture and Photosynthetic Activity

Low-temperature stress is the main limiting factor of cucurbit crop cultivation as it affects crop yield and quality. The identification of genes involved in cold tolerance is a crucial aspect of pumpkin rootstock breeding. Here, we examined the function of a pumpkin Regulator of Chromosome Condensa...

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Autores principales:	Mengmeng Wang, Shu Zhou, Junyang Lu, Anqi Xu, Yuan Huang, Zhilong Bie, Fei Cheng
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	CmRCC1 cold stress root architecture photosynthesis pumpkin Plant culture SB1-1110
Acceso en línea:	https://doaj.org/article/3f1f895e727a41618b321a37b2476752
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spelling	oai:doaj.org-article:3f1f895e727a41618b321a37b24767522021-12-03T07:19:56ZCmRCC1 Gene From Pumpkin Confers Cold Tolerance in Tobacco by Modulating Root Architecture and Photosynthetic Activity1664-462X10.3389/fpls.2021.765302https://doaj.org/article/3f1f895e727a41618b321a37b24767522021-12-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fpls.2021.765302/fullhttps://doaj.org/toc/1664-462XLow-temperature stress is the main limiting factor of cucurbit crop cultivation as it affects crop yield and quality. The identification of genes involved in cold tolerance is a crucial aspect of pumpkin rootstock breeding. Here, we examined the function of a pumpkin Regulator of Chromosome Condensation 1 (CmRCC1) gene in the root development and cold stress responses of tobacco (Nicotiana benthamiana). CmRCC1 expression was differentially induced in pumpkin root, stem, and leaf under cold stress. Transient transformation showed that CmRCC1 is located in the nucleus. CmRCC1 overexpression in tobacco increased the gravitropic set-point angle in lateral roots, as well as root diameter and volume. The expression of auxin polar transport factors, PIN1 and PIN3, decreased and increased in CmRCC1-overexpressed plants, respectively. Yeast two-hybrid verification and luciferase complementation imaging assay showed that CmRCC1 interacts with CmLAZY1. Furthermore, the decreases in maximum quantum yield of PS II, the effective quantum yield of PS II, and electron transfer rate and the increases in quantum yield of nonregulated energy dissipation and malondialdehyde content were compromised in transgenic plants compared with wild-type plants under cold stress. The results suggest that CmRCC1 plays an important role in the regulation of root architecture and positively modulates cold tolerance.Mengmeng WangShu ZhouJunyang LuAnqi XuYuan HuangZhilong BieFei ChengFrontiers Media S.A.articleCmRCC1cold stressroot architecturephotosynthesispumpkinPlant cultureSB1-1110ENFrontiers in Plant Science, Vol 12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	CmRCC1 cold stress root architecture photosynthesis pumpkin Plant culture SB1-1110
spellingShingle	CmRCC1 cold stress root architecture photosynthesis pumpkin Plant culture SB1-1110 Mengmeng Wang Shu Zhou Junyang Lu Anqi Xu Yuan Huang Zhilong Bie Fei Cheng CmRCC1 Gene From Pumpkin Confers Cold Tolerance in Tobacco by Modulating Root Architecture and Photosynthetic Activity
description	Low-temperature stress is the main limiting factor of cucurbit crop cultivation as it affects crop yield and quality. The identification of genes involved in cold tolerance is a crucial aspect of pumpkin rootstock breeding. Here, we examined the function of a pumpkin Regulator of Chromosome Condensation 1 (CmRCC1) gene in the root development and cold stress responses of tobacco (Nicotiana benthamiana). CmRCC1 expression was differentially induced in pumpkin root, stem, and leaf under cold stress. Transient transformation showed that CmRCC1 is located in the nucleus. CmRCC1 overexpression in tobacco increased the gravitropic set-point angle in lateral roots, as well as root diameter and volume. The expression of auxin polar transport factors, PIN1 and PIN3, decreased and increased in CmRCC1-overexpressed plants, respectively. Yeast two-hybrid verification and luciferase complementation imaging assay showed that CmRCC1 interacts with CmLAZY1. Furthermore, the decreases in maximum quantum yield of PS II, the effective quantum yield of PS II, and electron transfer rate and the increases in quantum yield of nonregulated energy dissipation and malondialdehyde content were compromised in transgenic plants compared with wild-type plants under cold stress. The results suggest that CmRCC1 plays an important role in the regulation of root architecture and positively modulates cold tolerance.
format	article
author	Mengmeng Wang Shu Zhou Junyang Lu Anqi Xu Yuan Huang Zhilong Bie Fei Cheng
author_facet	Mengmeng Wang Shu Zhou Junyang Lu Anqi Xu Yuan Huang Zhilong Bie Fei Cheng
author_sort	Mengmeng Wang
title	CmRCC1 Gene From Pumpkin Confers Cold Tolerance in Tobacco by Modulating Root Architecture and Photosynthetic Activity
title_short	CmRCC1 Gene From Pumpkin Confers Cold Tolerance in Tobacco by Modulating Root Architecture and Photosynthetic Activity
title_full	CmRCC1 Gene From Pumpkin Confers Cold Tolerance in Tobacco by Modulating Root Architecture and Photosynthetic Activity
title_fullStr	CmRCC1 Gene From Pumpkin Confers Cold Tolerance in Tobacco by Modulating Root Architecture and Photosynthetic Activity
title_full_unstemmed	CmRCC1 Gene From Pumpkin Confers Cold Tolerance in Tobacco by Modulating Root Architecture and Photosynthetic Activity
title_sort	cmrcc1 gene from pumpkin confers cold tolerance in tobacco by modulating root architecture and photosynthetic activity
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/3f1f895e727a41618b321a37b2476752
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_version_	1718373804675170304

CmRCC1 Gene From Pumpkin Confers Cold Tolerance in Tobacco by Modulating Root Architecture and Photosynthetic Activity

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