Cuticle deposition duration in the uterus is correlated with eggshell cuticle quality in White Leghorn laying hens

Abstract The cuticle formed in the uterus is the outermost layer as the first defense line of eggshell against microbial invasions in most avian species, and analyzing its genetic regulation and influencing factors are of great importance to egg biosecurity in poultry production worldwide. The curre...

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Autores principales:	Xia Chen, Zhaoxiang He, Xingzheng Li, Jianlou Song, Mingyi Huang, Xuefeng Shi, Xianyu Li, Junying Li, Guiyun Xu, Jiangxia Zheng
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/3f217435cc744ab5b67c738847bd904d
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spelling	oai:doaj.org-article:3f217435cc744ab5b67c738847bd904d2021-11-14T12:20:42ZCuticle deposition duration in the uterus is correlated with eggshell cuticle quality in White Leghorn laying hens10.1038/s41598-021-01718-02045-2322https://doaj.org/article/3f217435cc744ab5b67c738847bd904d2021-11-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-01718-0https://doaj.org/toc/2045-2322Abstract The cuticle formed in the uterus is the outermost layer as the first defense line of eggshell against microbial invasions in most avian species, and analyzing its genetic regulation and influencing factors are of great importance to egg biosecurity in poultry production worldwide. The current study compared the uterine transcriptome and proteome of laying hens producing eggs with good and poor cuticle quality (GC and PC, the top and tail of the cuticle quality distribution), and identified several genes involved with eggshell cuticle quality (ESCQ). Overall, transcriptomic analysis identified 53 differentially expressed genes (DEGs) between PC versus GC group hens, among which 25 were up-regulated and 28 were down-regulated. No differences were found in the uterine proteome. Several DEGs, including PTGDS, PLCG2, ADM and PRLR related to uterine functions and reproductive hormones, were validated by qPCR analysis. Egg quality measurements between GC and PC hens showed GC hens had longer laying interval between two consecutive ovipositions (25.64 ± 1.23 vs 24.94 ± 1.12 h) and thicker eggshell thickness (352.01 ± 23.04 vs 316.20 ± 30.58 μm) (P < 0.05). Apart from eggshell traits, other egg quality traits didn’t differ. The result demonstrated eggshell and cuticle deposition duration in the uterus is one of the major factors affecting ESCQ in laying hens. PTGDS, PLCG2, ADM and PRLR genes were discovered and might play crucial roles in cuticle deposition by regulating the uterine muscular activities and secretion function. The findings in the present study provide new insights into the genetic regulation of cuticle deposition in laying hens and establish a foundation for further investigations.Xia ChenZhaoxiang HeXingzheng LiJianlou SongMingyi HuangXuefeng ShiXianyu LiJunying LiGuiyun XuJiangxia ZhengNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Xia Chen Zhaoxiang He Xingzheng Li Jianlou Song Mingyi Huang Xuefeng Shi Xianyu Li Junying Li Guiyun Xu Jiangxia Zheng Cuticle deposition duration in the uterus is correlated with eggshell cuticle quality in White Leghorn laying hens
description	Abstract The cuticle formed in the uterus is the outermost layer as the first defense line of eggshell against microbial invasions in most avian species, and analyzing its genetic regulation and influencing factors are of great importance to egg biosecurity in poultry production worldwide. The current study compared the uterine transcriptome and proteome of laying hens producing eggs with good and poor cuticle quality (GC and PC, the top and tail of the cuticle quality distribution), and identified several genes involved with eggshell cuticle quality (ESCQ). Overall, transcriptomic analysis identified 53 differentially expressed genes (DEGs) between PC versus GC group hens, among which 25 were up-regulated and 28 were down-regulated. No differences were found in the uterine proteome. Several DEGs, including PTGDS, PLCG2, ADM and PRLR related to uterine functions and reproductive hormones, were validated by qPCR analysis. Egg quality measurements between GC and PC hens showed GC hens had longer laying interval between two consecutive ovipositions (25.64 ± 1.23 vs 24.94 ± 1.12 h) and thicker eggshell thickness (352.01 ± 23.04 vs 316.20 ± 30.58 μm) (P < 0.05). Apart from eggshell traits, other egg quality traits didn’t differ. The result demonstrated eggshell and cuticle deposition duration in the uterus is one of the major factors affecting ESCQ in laying hens. PTGDS, PLCG2, ADM and PRLR genes were discovered and might play crucial roles in cuticle deposition by regulating the uterine muscular activities and secretion function. The findings in the present study provide new insights into the genetic regulation of cuticle deposition in laying hens and establish a foundation for further investigations.
format	article
author	Xia Chen Zhaoxiang He Xingzheng Li Jianlou Song Mingyi Huang Xuefeng Shi Xianyu Li Junying Li Guiyun Xu Jiangxia Zheng
author_facet	Xia Chen Zhaoxiang He Xingzheng Li Jianlou Song Mingyi Huang Xuefeng Shi Xianyu Li Junying Li Guiyun Xu Jiangxia Zheng
author_sort	Xia Chen
title	Cuticle deposition duration in the uterus is correlated with eggshell cuticle quality in White Leghorn laying hens
title_short	Cuticle deposition duration in the uterus is correlated with eggshell cuticle quality in White Leghorn laying hens
title_full	Cuticle deposition duration in the uterus is correlated with eggshell cuticle quality in White Leghorn laying hens
title_fullStr	Cuticle deposition duration in the uterus is correlated with eggshell cuticle quality in White Leghorn laying hens
title_full_unstemmed	Cuticle deposition duration in the uterus is correlated with eggshell cuticle quality in White Leghorn laying hens
title_sort	cuticle deposition duration in the uterus is correlated with eggshell cuticle quality in white leghorn laying hens
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/3f217435cc744ab5b67c738847bd904d
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Cuticle deposition duration in the uterus is correlated with eggshell cuticle quality in White Leghorn laying hens

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