Characterization of Anatomical and Physiological Effects of Variegation Mutation on Grapevine

Variegation is a common trait in plants that characteristically displays white or off-colored plant tissue. In grapevine, leaf variegation is expressed as white and pale green leaf tissue resulting in plants that are stunted in growth and hindered in development. In this study, several experiments w...

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Autores principales:	Jack Olson, Matthew Clark
Formato:	article
Lenguaje:	EN
Publicado:	American Society for Horticultural Science (ASHS) 2021
Materias:	chlorophyll chloroplast grape histology plant cell vitis Plant culture SB1-1110
Acceso en línea:	https://doi.org/10.21273/HORTSCI15929-21 https://doaj.org/article/3f223dd3ec1244479a678392574202c0
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spelling	oai:doaj.org-article:3f223dd3ec1244479a678392574202c02021-11-08T18:31:54ZCharacterization of Anatomical and Physiological Effects of Variegation Mutation on Grapevinehttps://doi.org/10.21273/HORTSCI15929-212327-9834https://doaj.org/article/3f223dd3ec1244479a678392574202c02021-09-01T00:00:00Zhttps://journals.ashs.org/hortsci/view/journals/hortsci/56/10/article-p1251.xmlhttps://doaj.org/toc/2327-9834Variegation is a common trait in plants that characteristically displays white or off-colored plant tissue. In grapevine, leaf variegation is expressed as white and pale green leaf tissue resulting in plants that are stunted in growth and hindered in development. In this study, several experiments were performed to investigate the impact of this mutation has on the anatomy of grape leaves and physiology of the plant. Histological staining of variegated and nonvariegated leaf tissue transections showed alterations to the leaf palisade mesophyll structure that affected leaf tissue width. An assay quantifying leaf pigments was performed to compare chlorophyll and carotenoid concentrations in leaves between variegated and wild-type seedlings, which showed that variegated leaf samples had reduced chlorophyll and carotenoid concentration. Through fluorescence imaging, we determined that photochemical efficiency of photosystem II (PSII) is reduced in variegated seedlings. By growing variegated and wild-type plants under high, medium, and low light intensities that variegated plants exposed to higher light intensity reduces the phenotypic expression of the variegation trait. Also, we found variegated plants to have significant reductions in growth traits such as plant height, leaf number, branch number, and dry weight compared with wild-type phenotype plants. Overall, our experiments revealed the variegation mutation altered normal leaf development causing significant effects to grapevine physiology.Jack OlsonMatthew ClarkAmerican Society for Horticultural Science (ASHS)articlechlorophyllchloroplastgrapehistologyplant cellvitisPlant cultureSB1-1110ENHortScience, Vol 56, Iss 10, Pp 1251-1257 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	chlorophyll chloroplast grape histology plant cell vitis Plant culture SB1-1110
spellingShingle	chlorophyll chloroplast grape histology plant cell vitis Plant culture SB1-1110 Jack Olson Matthew Clark Characterization of Anatomical and Physiological Effects of Variegation Mutation on Grapevine
description	Variegation is a common trait in plants that characteristically displays white or off-colored plant tissue. In grapevine, leaf variegation is expressed as white and pale green leaf tissue resulting in plants that are stunted in growth and hindered in development. In this study, several experiments were performed to investigate the impact of this mutation has on the anatomy of grape leaves and physiology of the plant. Histological staining of variegated and nonvariegated leaf tissue transections showed alterations to the leaf palisade mesophyll structure that affected leaf tissue width. An assay quantifying leaf pigments was performed to compare chlorophyll and carotenoid concentrations in leaves between variegated and wild-type seedlings, which showed that variegated leaf samples had reduced chlorophyll and carotenoid concentration. Through fluorescence imaging, we determined that photochemical efficiency of photosystem II (PSII) is reduced in variegated seedlings. By growing variegated and wild-type plants under high, medium, and low light intensities that variegated plants exposed to higher light intensity reduces the phenotypic expression of the variegation trait. Also, we found variegated plants to have significant reductions in growth traits such as plant height, leaf number, branch number, and dry weight compared with wild-type phenotype plants. Overall, our experiments revealed the variegation mutation altered normal leaf development causing significant effects to grapevine physiology.
format	article
author	Jack Olson Matthew Clark
author_facet	Jack Olson Matthew Clark
author_sort	Jack Olson
title	Characterization of Anatomical and Physiological Effects of Variegation Mutation on Grapevine
title_short	Characterization of Anatomical and Physiological Effects of Variegation Mutation on Grapevine
title_full	Characterization of Anatomical and Physiological Effects of Variegation Mutation on Grapevine
title_fullStr	Characterization of Anatomical and Physiological Effects of Variegation Mutation on Grapevine
title_full_unstemmed	Characterization of Anatomical and Physiological Effects of Variegation Mutation on Grapevine
title_sort	characterization of anatomical and physiological effects of variegation mutation on grapevine
publisher	American Society for Horticultural Science (ASHS)
publishDate	2021
url	https://doi.org/10.21273/HORTSCI15929-21 https://doaj.org/article/3f223dd3ec1244479a678392574202c0
work_keys_str_mv	AT jackolson characterizationofanatomicalandphysiologicaleffectsofvariegationmutationongrapevine AT matthewclark characterizationofanatomicalandphysiologicaleffectsofvariegationmutationongrapevine
_version_	1718441463392501760

Characterization of Anatomical and Physiological Effects of Variegation Mutation on Grapevine

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