Laboratory Activity Using Accessible Microfluidics to Study Nematode Behavior in an Electrical Field

Microfluidic devices are used in a broad range of technological applications, from creating ingredients for cosmetics to discovering new medicines. The small size of microfluidic channels makes it possible to isolate individual cells, collections of cells, and multicellular organisms and study their...

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Autores principales:	Elizabeth D. Clawson, Val Blair, Julia F. Nepper, Matthew D. Stilwell, Travis Tangen, Douglas B. Weibel
Formato:	article
Lenguaje:	EN
Publicado:	American Society for Microbiology 2018
Materias:	Special aspects of education LC8-6691 Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/3f2ce848e15a470ca4c6fe9f2c897dd2
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spelling	oai:doaj.org-article:3f2ce848e15a470ca4c6fe9f2c897dd22021-11-15T15:04:53ZLaboratory Activity Using Accessible Microfluidics to Study Nematode Behavior in an Electrical Field10.1128/jmbe.v19i1.15511935-78851935-7877https://doaj.org/article/3f2ce848e15a470ca4c6fe9f2c897dd22018-03-01T00:00:00Zhttps://journals.asm.org/doi/10.1128/jmbe.v19i1.1551https://doaj.org/toc/1935-7877https://doaj.org/toc/1935-7885Microfluidic devices are used in a broad range of technological applications, from creating ingredients for cosmetics to discovering new medicines. The small size of microfluidic channels makes it possible to isolate individual cells, collections of cells, and multicellular organisms and study their biology, ecology, and behavior. Microfluidics is particularly well suited to teaching students concepts from different fields of science. A challenge with conventional microfluidic devices is that they are difficult and expensive to make, which has been a barrier for their entry into curricula and classrooms. We describe a simple and low-cost method for creating microfluidic devices and use them to study the behavior of nematodes in an electrical field. Nematodes are ecologically and agriculturally important organisms that respond robustly to various environmental cues. In this activity, we demonstrate that nematodes swim through liquid in microfluidic channels in response to an applied electric field and describe student responses to this activity.Elizabeth D. ClawsonVal BlairJulia F. NepperMatthew D. StilwellTravis TangenDouglas B. WeibelAmerican Society for MicrobiologyarticleSpecial aspects of educationLC8-6691Biology (General)QH301-705.5ENJournal of Microbiology & Biology Education, Vol 19, Iss 1 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Special aspects of education LC8-6691 Biology (General) QH301-705.5
spellingShingle	Special aspects of education LC8-6691 Biology (General) QH301-705.5 Elizabeth D. Clawson Val Blair Julia F. Nepper Matthew D. Stilwell Travis Tangen Douglas B. Weibel Laboratory Activity Using Accessible Microfluidics to Study Nematode Behavior in an Electrical Field
description	Microfluidic devices are used in a broad range of technological applications, from creating ingredients for cosmetics to discovering new medicines. The small size of microfluidic channels makes it possible to isolate individual cells, collections of cells, and multicellular organisms and study their biology, ecology, and behavior. Microfluidics is particularly well suited to teaching students concepts from different fields of science. A challenge with conventional microfluidic devices is that they are difficult and expensive to make, which has been a barrier for their entry into curricula and classrooms. We describe a simple and low-cost method for creating microfluidic devices and use them to study the behavior of nematodes in an electrical field. Nematodes are ecologically and agriculturally important organisms that respond robustly to various environmental cues. In this activity, we demonstrate that nematodes swim through liquid in microfluidic channels in response to an applied electric field and describe student responses to this activity.
format	article
author	Elizabeth D. Clawson Val Blair Julia F. Nepper Matthew D. Stilwell Travis Tangen Douglas B. Weibel
author_facet	Elizabeth D. Clawson Val Blair Julia F. Nepper Matthew D. Stilwell Travis Tangen Douglas B. Weibel
author_sort	Elizabeth D. Clawson
title	Laboratory Activity Using Accessible Microfluidics to Study Nematode Behavior in an Electrical Field
title_short	Laboratory Activity Using Accessible Microfluidics to Study Nematode Behavior in an Electrical Field
title_full	Laboratory Activity Using Accessible Microfluidics to Study Nematode Behavior in an Electrical Field
title_fullStr	Laboratory Activity Using Accessible Microfluidics to Study Nematode Behavior in an Electrical Field
title_full_unstemmed	Laboratory Activity Using Accessible Microfluidics to Study Nematode Behavior in an Electrical Field
title_sort	laboratory activity using accessible microfluidics to study nematode behavior in an electrical field
publisher	American Society for Microbiology
publishDate	2018
url	https://doaj.org/article/3f2ce848e15a470ca4c6fe9f2c897dd2
work_keys_str_mv	AT elizabethdclawson laboratoryactivityusingaccessiblemicrofluidicstostudynematodebehaviorinanelectricalfield AT valblair laboratoryactivityusingaccessiblemicrofluidicstostudynematodebehaviorinanelectricalfield AT juliafnepper laboratoryactivityusingaccessiblemicrofluidicstostudynematodebehaviorinanelectricalfield AT matthewdstilwell laboratoryactivityusingaccessiblemicrofluidicstostudynematodebehaviorinanelectricalfield AT travistangen laboratoryactivityusingaccessiblemicrofluidicstostudynematodebehaviorinanelectricalfield AT douglasbweibel laboratoryactivityusingaccessiblemicrofluidicstostudynematodebehaviorinanelectricalfield
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Laboratory Activity Using Accessible Microfluidics to Study Nematode Behavior in an Electrical Field

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