Conserved and divergent roles of Bcr1 and CFEM proteins in Candida parapsilosis and Candida albicans.

Candida parapsilosis is a pathogenic fungus that is major cause of hospital-acquired infection, predominantly due to growth as biofilms on indwelling medical devices. It is related to Candida albicans, which remains the most common cause of candidiasis disease in humans. The transcription factor Bcr...

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Autores principales:	Chen Ding, Genevieve M Vidanes, Sarah L Maguire, Alessandro Guida, John M Synnott, David R Andes, Geraldine Butler
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2011
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/3f2e593a1db246a281b806796846c953
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spelling	oai:doaj.org-article:3f2e593a1db246a281b806796846c9532021-11-18T07:33:17ZConserved and divergent roles of Bcr1 and CFEM proteins in Candida parapsilosis and Candida albicans.1932-620310.1371/journal.pone.0028151https://doaj.org/article/3f2e593a1db246a281b806796846c9532011-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22145027/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203Candida parapsilosis is a pathogenic fungus that is major cause of hospital-acquired infection, predominantly due to growth as biofilms on indwelling medical devices. It is related to Candida albicans, which remains the most common cause of candidiasis disease in humans. The transcription factor Bcr1 is an important regulator of biofilm formation in vitro in both C. parapsilosis and C. albicans. We show here that C. parapsilosis Bcr1 is required for in vivo biofilm development in a rat catheter model, like C. albicans. By comparing the transcription profiles of a bcr1 deletion in both species we found that regulation of expression of the CFEM family is conserved. In C. albicans, three of the five CFEM cell wall proteins (Rbt5, Pga7 and Csa1) are associated with both biofilm formation and acquisition of iron from heme, which is an important virulence characteristic. In C. parapsilosis, the CFEM family has undergone an expansion to 7 members. Expression of three genes (CFEM2, CFEM3, and CFEM6) is dependent on Bcr1, and is induced in low iron conditions. All three are involved in the acquisition of iron from heme. However, deletion of the three CFEM genes has no effect on biofilm formation in C. parapsilosis. Our data suggest that the role of the CFEM family in iron acquisition is conserved between C. albicans and C. parapsilosis, but their role in biofilm formation is not.Chen DingGenevieve M VidanesSarah L MaguireAlessandro GuidaJohn M SynnottDavid R AndesGeraldine ButlerPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 6, Iss 12, p e28151 (2011)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Chen Ding Genevieve M Vidanes Sarah L Maguire Alessandro Guida John M Synnott David R Andes Geraldine Butler Conserved and divergent roles of Bcr1 and CFEM proteins in Candida parapsilosis and Candida albicans.
description	Candida parapsilosis is a pathogenic fungus that is major cause of hospital-acquired infection, predominantly due to growth as biofilms on indwelling medical devices. It is related to Candida albicans, which remains the most common cause of candidiasis disease in humans. The transcription factor Bcr1 is an important regulator of biofilm formation in vitro in both C. parapsilosis and C. albicans. We show here that C. parapsilosis Bcr1 is required for in vivo biofilm development in a rat catheter model, like C. albicans. By comparing the transcription profiles of a bcr1 deletion in both species we found that regulation of expression of the CFEM family is conserved. In C. albicans, three of the five CFEM cell wall proteins (Rbt5, Pga7 and Csa1) are associated with both biofilm formation and acquisition of iron from heme, which is an important virulence characteristic. In C. parapsilosis, the CFEM family has undergone an expansion to 7 members. Expression of three genes (CFEM2, CFEM3, and CFEM6) is dependent on Bcr1, and is induced in low iron conditions. All three are involved in the acquisition of iron from heme. However, deletion of the three CFEM genes has no effect on biofilm formation in C. parapsilosis. Our data suggest that the role of the CFEM family in iron acquisition is conserved between C. albicans and C. parapsilosis, but their role in biofilm formation is not.
format	article
author	Chen Ding Genevieve M Vidanes Sarah L Maguire Alessandro Guida John M Synnott David R Andes Geraldine Butler
author_facet	Chen Ding Genevieve M Vidanes Sarah L Maguire Alessandro Guida John M Synnott David R Andes Geraldine Butler
author_sort	Chen Ding
title	Conserved and divergent roles of Bcr1 and CFEM proteins in Candida parapsilosis and Candida albicans.
title_short	Conserved and divergent roles of Bcr1 and CFEM proteins in Candida parapsilosis and Candida albicans.
title_full	Conserved and divergent roles of Bcr1 and CFEM proteins in Candida parapsilosis and Candida albicans.
title_fullStr	Conserved and divergent roles of Bcr1 and CFEM proteins in Candida parapsilosis and Candida albicans.
title_full_unstemmed	Conserved and divergent roles of Bcr1 and CFEM proteins in Candida parapsilosis and Candida albicans.
title_sort	conserved and divergent roles of bcr1 and cfem proteins in candida parapsilosis and candida albicans.
publisher	Public Library of Science (PLoS)
publishDate	2011
url	https://doaj.org/article/3f2e593a1db246a281b806796846c953
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Conserved and divergent roles of Bcr1 and CFEM proteins in Candida parapsilosis and Candida albicans.

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