Terahertz Spectroscopic Diagnosis of Myelin Deficit Brain in Mice and Rhesus Monkey with Chemometric Techniques

Abstract While myelin deficit of the central nervous system leads to several severe diseases, the definitive diagnostic means are lacking. We proposed and performed terahertz time-domain spectroscopy (THz-TDS) combined with chemometric techniques to discriminate and evaluate the severity of myelin d...

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Autores principales:	Yi Zou, Jiang Li, Yiyuan Cui, Peiren Tang, Lianghui Du, Tunan Chen, Kun Meng, Qiao Liu, Hua Feng, Jianheng Zhao, Mina Chen, Li-Guo Zhu
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/3f3d22f40e51404fab398bb26bb0e490
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spelling	oai:doaj.org-article:3f3d22f40e51404fab398bb26bb0e4902021-12-02T12:31:48ZTerahertz Spectroscopic Diagnosis of Myelin Deficit Brain in Mice and Rhesus Monkey with Chemometric Techniques10.1038/s41598-017-05554-z2045-2322https://doaj.org/article/3f3d22f40e51404fab398bb26bb0e4902017-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-05554-zhttps://doaj.org/toc/2045-2322Abstract While myelin deficit of the central nervous system leads to several severe diseases, the definitive diagnostic means are lacking. We proposed and performed terahertz time-domain spectroscopy (THz-TDS) combined with chemometric techniques to discriminate and evaluate the severity of myelin deficit in mouse and rhesus monkey brains. The THz refractive index and absorption coefficient of paraffin-embedded brain tissues from both normal and mutant dysmyelinating mice are shown. Principal component analysis of time-domain THz signal (PCA-tdTHz) and absorption-refractive index relation of THz spectrum identified myelin deficit without exogenous labeling or any pretreatment. Further, with the established PCA-tdTHz, we evaluated the severity of myelin deficit lesions in rhesus monkey brain induced by experimental autoimmune encephalomyelitis, which is the most-studied animal model of multiple sclerosis. The results well matched the pathological analysis, indicating that PCA-tdTHz is a quick, powerful, evolving tool for identification and evaluation myelin deficit in preclinical animals and potentially in para-clinical human biopsy.Yi ZouJiang LiYiyuan CuiPeiren TangLianghui DuTunan ChenKun MengQiao LiuHua FengJianheng ZhaoMina ChenLi-Guo ZhuNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-9 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Yi Zou Jiang Li Yiyuan Cui Peiren Tang Lianghui Du Tunan Chen Kun Meng Qiao Liu Hua Feng Jianheng Zhao Mina Chen Li-Guo Zhu Terahertz Spectroscopic Diagnosis of Myelin Deficit Brain in Mice and Rhesus Monkey with Chemometric Techniques
description	Abstract While myelin deficit of the central nervous system leads to several severe diseases, the definitive diagnostic means are lacking. We proposed and performed terahertz time-domain spectroscopy (THz-TDS) combined with chemometric techniques to discriminate and evaluate the severity of myelin deficit in mouse and rhesus monkey brains. The THz refractive index and absorption coefficient of paraffin-embedded brain tissues from both normal and mutant dysmyelinating mice are shown. Principal component analysis of time-domain THz signal (PCA-tdTHz) and absorption-refractive index relation of THz spectrum identified myelin deficit without exogenous labeling or any pretreatment. Further, with the established PCA-tdTHz, we evaluated the severity of myelin deficit lesions in rhesus monkey brain induced by experimental autoimmune encephalomyelitis, which is the most-studied animal model of multiple sclerosis. The results well matched the pathological analysis, indicating that PCA-tdTHz is a quick, powerful, evolving tool for identification and evaluation myelin deficit in preclinical animals and potentially in para-clinical human biopsy.
format	article
author	Yi Zou Jiang Li Yiyuan Cui Peiren Tang Lianghui Du Tunan Chen Kun Meng Qiao Liu Hua Feng Jianheng Zhao Mina Chen Li-Guo Zhu
author_facet	Yi Zou Jiang Li Yiyuan Cui Peiren Tang Lianghui Du Tunan Chen Kun Meng Qiao Liu Hua Feng Jianheng Zhao Mina Chen Li-Guo Zhu
author_sort	Yi Zou
title	Terahertz Spectroscopic Diagnosis of Myelin Deficit Brain in Mice and Rhesus Monkey with Chemometric Techniques
title_short	Terahertz Spectroscopic Diagnosis of Myelin Deficit Brain in Mice and Rhesus Monkey with Chemometric Techniques
title_full	Terahertz Spectroscopic Diagnosis of Myelin Deficit Brain in Mice and Rhesus Monkey with Chemometric Techniques
title_fullStr	Terahertz Spectroscopic Diagnosis of Myelin Deficit Brain in Mice and Rhesus Monkey with Chemometric Techniques
title_full_unstemmed	Terahertz Spectroscopic Diagnosis of Myelin Deficit Brain in Mice and Rhesus Monkey with Chemometric Techniques
title_sort	terahertz spectroscopic diagnosis of myelin deficit brain in mice and rhesus monkey with chemometric techniques
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/3f3d22f40e51404fab398bb26bb0e490
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Terahertz Spectroscopic Diagnosis of Myelin Deficit Brain in Mice and Rhesus Monkey with Chemometric Techniques

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