Persulfidation of Nitrate Reductase 2 Is Involved in l-Cysteine Desulfhydrase-Regulated Rice Drought Tolerance

Hydrogen sulfide (H2S) is an important signaling molecule that regulates diverse cellular signaling pathways through persulfidation. Our previous study revealed that H2S is involved in the improvement of rice drought tolerance. However, the corresponding...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Heng Zhou, Yin Zhou, Feng Zhang, Wenxue Guan, Ye Su, Xingxing Yuan, Yanjie Xie
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	hydrogen sulfide persulfidation drought stress nitrate reductase <span style="font-variant: small-caps">l</span>-cysteine desulfhydrase Biology (General) QH301-705.5 Chemistry QD1-999
Acceso en línea:	https://doaj.org/article/3f42c4ec2a9c4ca19abbe6186d9a10f5
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:3f42c4ec2a9c4ca19abbe6186d9a10f5
record_format	dspace
spelling	oai:doaj.org-article:3f42c4ec2a9c4ca19abbe6186d9a10f52021-11-25T17:53:32ZPersulfidation of Nitrate Reductase 2 Is Involved in <span style="font-variant: small-caps">l</span>-Cysteine Desulfhydrase-Regulated Rice Drought Tolerance10.3390/ijms2222121191422-00671661-6596https://doaj.org/article/3f42c4ec2a9c4ca19abbe6186d9a10f52021-11-01T00:00:00Zhttps://www.mdpi.com/1422-0067/22/22/12119https://doaj.org/toc/1661-6596https://doaj.org/toc/1422-0067Hydrogen sulfide (H<sub>2</sub>S) is an important signaling molecule that regulates diverse cellular signaling pathways through persulfidation. Our previous study revealed that H<sub>2</sub>S is involved in the improvement of rice drought tolerance. However, the corresponding enzymatic sources of H<sub>2</sub>S and its regulatory mechanism in response to drought stress are not clear. Here, we cloned and characterized a putative <i><span style="font-variant: small-caps;">l</span>-cysteine desulfhydrase</i> (<i>LCD</i>) gene in rice, which encodes a protein possessing H<sub>2</sub>S-producing activity and was named <i>OsLCD1</i>. Overexpression of <i>OsLCD1</i> results in enhanced H<sub>2</sub>S production, persulfidation of total soluble protein, and confers rice drought tolerance. Further, we found that nitrate reductase (NR) activity was decreased under drought stress, and the inhibition of NR activity was controlled by endogenous H<sub>2</sub>S production. Persulfidation of NIA2, an NR isoform responsible for the main NR activity, led to a decrease in total NR activity in rice. Furthermore, drought stress-triggered inhibition of NR activity and persulfidation of NIA2 was intensified in the <i>OsLCD1</i> overexpression line. Phenotypical and molecular analysis revealed that mutation of <i>NIA2</i> enhanced rice drought tolerance by activating the expression of genes encoding antioxidant enzymes and ABA-responsive genes. Taken together, our results showed the role of <i>OsLCD1</i> in modulating H<sub>2</sub>S production and provided insight into H<sub>2</sub>S-regulated persulfidation of NIA2 in the control of rice drought stress.Heng ZhouYin ZhouFeng ZhangWenxue GuanYe SuXingxing YuanYanjie XieMDPI AGarticlehydrogen sulfidepersulfidationdrought stressnitrate reductase<span style="font-variant: small-caps">l</span>-cysteine desulfhydraseBiology (General)QH301-705.5ChemistryQD1-999ENInternational Journal of Molecular Sciences, Vol 22, Iss 12119, p 12119 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	hydrogen sulfide persulfidation drought stress nitrate reductase <span style="font-variant: small-caps">l</span>-cysteine desulfhydrase Biology (General) QH301-705.5 Chemistry QD1-999
spellingShingle	hydrogen sulfide persulfidation drought stress nitrate reductase <span style="font-variant: small-caps">l</span>-cysteine desulfhydrase Biology (General) QH301-705.5 Chemistry QD1-999 Heng Zhou Yin Zhou Feng Zhang Wenxue Guan Ye Su Xingxing Yuan Yanjie Xie Persulfidation of Nitrate Reductase 2 Is Involved in <span style="font-variant: small-caps">l</span>-Cysteine Desulfhydrase-Regulated Rice Drought Tolerance
description	Hydrogen sulfide (H<sub>2</sub>S) is an important signaling molecule that regulates diverse cellular signaling pathways through persulfidation. Our previous study revealed that H<sub>2</sub>S is involved in the improvement of rice drought tolerance. However, the corresponding enzymatic sources of H<sub>2</sub>S and its regulatory mechanism in response to drought stress are not clear. Here, we cloned and characterized a putative <i><span style="font-variant: small-caps;">l</span>-cysteine desulfhydrase</i> (<i>LCD</i>) gene in rice, which encodes a protein possessing H<sub>2</sub>S-producing activity and was named <i>OsLCD1</i>. Overexpression of <i>OsLCD1</i> results in enhanced H<sub>2</sub>S production, persulfidation of total soluble protein, and confers rice drought tolerance. Further, we found that nitrate reductase (NR) activity was decreased under drought stress, and the inhibition of NR activity was controlled by endogenous H<sub>2</sub>S production. Persulfidation of NIA2, an NR isoform responsible for the main NR activity, led to a decrease in total NR activity in rice. Furthermore, drought stress-triggered inhibition of NR activity and persulfidation of NIA2 was intensified in the <i>OsLCD1</i> overexpression line. Phenotypical and molecular analysis revealed that mutation of <i>NIA2</i> enhanced rice drought tolerance by activating the expression of genes encoding antioxidant enzymes and ABA-responsive genes. Taken together, our results showed the role of <i>OsLCD1</i> in modulating H<sub>2</sub>S production and provided insight into H<sub>2</sub>S-regulated persulfidation of NIA2 in the control of rice drought stress.
format	article
author	Heng Zhou Yin Zhou Feng Zhang Wenxue Guan Ye Su Xingxing Yuan Yanjie Xie
author_facet	Heng Zhou Yin Zhou Feng Zhang Wenxue Guan Ye Su Xingxing Yuan Yanjie Xie
author_sort	Heng Zhou
title	Persulfidation of Nitrate Reductase 2 Is Involved in <span style="font-variant: small-caps">l</span>-Cysteine Desulfhydrase-Regulated Rice Drought Tolerance
title_short	Persulfidation of Nitrate Reductase 2 Is Involved in <span style="font-variant: small-caps">l</span>-Cysteine Desulfhydrase-Regulated Rice Drought Tolerance
title_full	Persulfidation of Nitrate Reductase 2 Is Involved in <span style="font-variant: small-caps">l</span>-Cysteine Desulfhydrase-Regulated Rice Drought Tolerance
title_fullStr	Persulfidation of Nitrate Reductase 2 Is Involved in <span style="font-variant: small-caps">l</span>-Cysteine Desulfhydrase-Regulated Rice Drought Tolerance
title_full_unstemmed	Persulfidation of Nitrate Reductase 2 Is Involved in <span style="font-variant: small-caps">l</span>-Cysteine Desulfhydrase-Regulated Rice Drought Tolerance
title_sort	persulfidation of nitrate reductase 2 is involved in <span style="font-variant: small-caps">l</span>-cysteine desulfhydrase-regulated rice drought tolerance
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/3f42c4ec2a9c4ca19abbe6186d9a10f5
work_keys_str_mv	AT hengzhou persulfidationofnitratereductase2isinvolvedinspanstylefontvariantsmallcapslspancysteinedesulfhydraseregulatedricedroughttolerance AT yinzhou persulfidationofnitratereductase2isinvolvedinspanstylefontvariantsmallcapslspancysteinedesulfhydraseregulatedricedroughttolerance AT fengzhang persulfidationofnitratereductase2isinvolvedinspanstylefontvariantsmallcapslspancysteinedesulfhydraseregulatedricedroughttolerance AT wenxueguan persulfidationofnitratereductase2isinvolvedinspanstylefontvariantsmallcapslspancysteinedesulfhydraseregulatedricedroughttolerance AT yesu persulfidationofnitratereductase2isinvolvedinspanstylefontvariantsmallcapslspancysteinedesulfhydraseregulatedricedroughttolerance AT xingxingyuan persulfidationofnitratereductase2isinvolvedinspanstylefontvariantsmallcapslspancysteinedesulfhydraseregulatedricedroughttolerance AT yanjiexie persulfidationofnitratereductase2isinvolvedinspanstylefontvariantsmallcapslspancysteinedesulfhydraseregulatedricedroughttolerance
_version_	1718411857842143232

Persulfidation of Nitrate Reductase 2 Is Involved in <span style="font-variant: small-caps">l</span>-Cysteine Desulfhydrase-Regulated Rice Drought Tolerance

Ejemplares similares