Comparison of polypeptides that bind the transferrin receptor for targeting gold nanocarriers.

The ability to target therapeutic agents to specific tissues is an important element in the development of new disease treatments. The transferrin receptor (TfR) is one potential target for drug delivery, as it expressed on many dividing cells and on brain endothelium, the key cellular component of...

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Autores principales:	Conor McQuaid, Andrea Halsey, Maëva Dubois, Ignacio Romero, David Male
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/3f44ca1c0c7b4826b97f016485872453
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spelling	oai:doaj.org-article:3f44ca1c0c7b4826b97f0164858724532021-11-25T06:23:35ZComparison of polypeptides that bind the transferrin receptor for targeting gold nanocarriers.1932-620310.1371/journal.pone.0252341https://doaj.org/article/3f44ca1c0c7b4826b97f0164858724532021-01-01T00:00:00Zhttps://doi.org/10.1371/journal.pone.0252341https://doaj.org/toc/1932-6203The ability to target therapeutic agents to specific tissues is an important element in the development of new disease treatments. The transferrin receptor (TfR) is one potential target for drug delivery, as it expressed on many dividing cells and on brain endothelium, the key cellular component of the blood-brain barrier. The aim of this study was to compare a set of new and previously-described polypeptides for their ability to bind to brain endothelium, and investigate their potential for targeting therapeutic agents to the CNS. Six polypeptides were ranked for their rate of endocytosis by the human brain endothelial cell line hCMEC/D3 and the murine line bEnd.3. One linear polypeptide and two cyclic polypeptides showed high rates of uptake. These peptides were investigated to determine whether serum components, including transferrin itself affected uptake by the endothelium. One of the cyclic peptides was strongly inhibited by transferrin and the other cyclic peptide weakly inhibited. As proof of principle the linear peptide was attached to 2nm glucose coated gold-nanoparticles, and the rate of uptake of the nanoparticles measured in a hydrogel model of the blood-brain barrier. Attachment of the TfR-targeting polypeptide significantly increased the rates of endocytosis by brain endothelium and increased movement of nanoparticles across the cells.Conor McQuaidAndrea HalseyMaëva DuboisIgnacio RomeroDavid MalePublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 16, Iss 6, p e0252341 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Conor McQuaid Andrea Halsey Maëva Dubois Ignacio Romero David Male Comparison of polypeptides that bind the transferrin receptor for targeting gold nanocarriers.
description	The ability to target therapeutic agents to specific tissues is an important element in the development of new disease treatments. The transferrin receptor (TfR) is one potential target for drug delivery, as it expressed on many dividing cells and on brain endothelium, the key cellular component of the blood-brain barrier. The aim of this study was to compare a set of new and previously-described polypeptides for their ability to bind to brain endothelium, and investigate their potential for targeting therapeutic agents to the CNS. Six polypeptides were ranked for their rate of endocytosis by the human brain endothelial cell line hCMEC/D3 and the murine line bEnd.3. One linear polypeptide and two cyclic polypeptides showed high rates of uptake. These peptides were investigated to determine whether serum components, including transferrin itself affected uptake by the endothelium. One of the cyclic peptides was strongly inhibited by transferrin and the other cyclic peptide weakly inhibited. As proof of principle the linear peptide was attached to 2nm glucose coated gold-nanoparticles, and the rate of uptake of the nanoparticles measured in a hydrogel model of the blood-brain barrier. Attachment of the TfR-targeting polypeptide significantly increased the rates of endocytosis by brain endothelium and increased movement of nanoparticles across the cells.
format	article
author	Conor McQuaid Andrea Halsey Maëva Dubois Ignacio Romero David Male
author_facet	Conor McQuaid Andrea Halsey Maëva Dubois Ignacio Romero David Male
author_sort	Conor McQuaid
title	Comparison of polypeptides that bind the transferrin receptor for targeting gold nanocarriers.
title_short	Comparison of polypeptides that bind the transferrin receptor for targeting gold nanocarriers.
title_full	Comparison of polypeptides that bind the transferrin receptor for targeting gold nanocarriers.
title_fullStr	Comparison of polypeptides that bind the transferrin receptor for targeting gold nanocarriers.
title_full_unstemmed	Comparison of polypeptides that bind the transferrin receptor for targeting gold nanocarriers.
title_sort	comparison of polypeptides that bind the transferrin receptor for targeting gold nanocarriers.
publisher	Public Library of Science (PLoS)
publishDate	2021
url	https://doaj.org/article/3f44ca1c0c7b4826b97f016485872453
work_keys_str_mv	AT conormcquaid comparisonofpolypeptidesthatbindthetransferrinreceptorfortargetinggoldnanocarriers AT andreahalsey comparisonofpolypeptidesthatbindthetransferrinreceptorfortargetinggoldnanocarriers AT maevadubois comparisonofpolypeptidesthatbindthetransferrinreceptorfortargetinggoldnanocarriers AT ignacioromero comparisonofpolypeptidesthatbindthetransferrinreceptorfortargetinggoldnanocarriers AT davidmale comparisonofpolypeptidesthatbindthetransferrinreceptorfortargetinggoldnanocarriers
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Comparison of polypeptides that bind the transferrin receptor for targeting gold nanocarriers.

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