Validation of Molecular Markers Genetically Linked to S-Cytoplasm and Restoration-of-fertility (Rf) Loci in Hot Pepper (Capsicum annuum L.)

Existence of CGMS system in hot pepper is due to the rearrangements in the mitochondrial genome and is largely used in economized and pure F 1 hybrid seed production around the world. The orf456, a new ORF present at flanking region of the coxII gene at the 3’ end, was distinguished male sterile cyt...

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Autores principales:	Jessy Mol K K, Lakshmana Reddy D C, Manoj Y B, Madhavi Reddy K
Formato:	article
Lenguaje:	EN
Publicado:	Society for Promotion of Horticulture - Indian Institute of Horticultural Research 2020
Materias:	cgms hot pepper marker assisted selection mitochondria orf Plant culture SB1-1110
Acceso en línea:	https://doaj.org/article/3f4a47056433400a9b1e60afc4ecf282
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spelling	oai:doaj.org-article:3f4a47056433400a9b1e60afc4ecf2822021-12-02T15:53:11ZValidation of Molecular Markers Genetically Linked to S-Cytoplasm and Restoration-of-fertility (Rf) Loci in Hot Pepper (Capsicum annuum L.)0973-354X2582-489910.24154/JHS.2020.v15i01.007https://doaj.org/article/3f4a47056433400a9b1e60afc4ecf2822020-06-01T00:00:00Zhttps://jhs.iihr.res.in/index.php/jhs/article/view/783https://doaj.org/toc/0973-354Xhttps://doaj.org/toc/2582-4899Existence of CGMS system in hot pepper is due to the rearrangements in the mitochondrial genome and is largely used in economized and pure F 1 hybrid seed production around the world. The orf456, a new ORF present at flanking region of the coxII gene at the 3’ end, was distinguished male sterile cytoplasm in hot peppers along with atp6-2gene. In the current study, eighteen pepper genotypes (nine each of A and corresponding B lines) of varied origin were used to validate with two male sterile cytoplasm (S-cytoplasm) specific sequence characterised amplified region (SCAR) markers viz., atp6-2 (875 bp) and orf456 (456 bp) and one restoration-of-fertility (Rf) locus specific marker, CRF (550 bp). The results clearly showed that the presence of CMS-S-cytoplasm and absence of restoration-of-fertility (Rf) gene in the pepper genotypes studied and is comparable with the phenotypic data. In view of the outcomes it has been reasoned that the accessible S and Rf markers available in the public domain are reproducible and can be promptly utilized for marker assisted selection (MAS) in hot pepper crop improvement program.Jessy Mol K KLakshmana Reddy D CManoj Y BMadhavi Reddy KSociety for Promotion of Horticulture - Indian Institute of Horticultural Researcharticlecgmshot peppermarker assisted selectionmitochondriaorfPlant cultureSB1-1110ENJournal of Horticultural Sciences, Vol 15, Iss 1, Pp 52-61 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	cgms hot pepper marker assisted selection mitochondria orf Plant culture SB1-1110
spellingShingle	cgms hot pepper marker assisted selection mitochondria orf Plant culture SB1-1110 Jessy Mol K K Lakshmana Reddy D C Manoj Y B Madhavi Reddy K Validation of Molecular Markers Genetically Linked to S-Cytoplasm and Restoration-of-fertility (Rf) Loci in Hot Pepper (Capsicum annuum L.)
description	Existence of CGMS system in hot pepper is due to the rearrangements in the mitochondrial genome and is largely used in economized and pure F 1 hybrid seed production around the world. The orf456, a new ORF present at flanking region of the coxII gene at the 3’ end, was distinguished male sterile cytoplasm in hot peppers along with atp6-2gene. In the current study, eighteen pepper genotypes (nine each of A and corresponding B lines) of varied origin were used to validate with two male sterile cytoplasm (S-cytoplasm) specific sequence characterised amplified region (SCAR) markers viz., atp6-2 (875 bp) and orf456 (456 bp) and one restoration-of-fertility (Rf) locus specific marker, CRF (550 bp). The results clearly showed that the presence of CMS-S-cytoplasm and absence of restoration-of-fertility (Rf) gene in the pepper genotypes studied and is comparable with the phenotypic data. In view of the outcomes it has been reasoned that the accessible S and Rf markers available in the public domain are reproducible and can be promptly utilized for marker assisted selection (MAS) in hot pepper crop improvement program.
format	article
author	Jessy Mol K K Lakshmana Reddy D C Manoj Y B Madhavi Reddy K
author_facet	Jessy Mol K K Lakshmana Reddy D C Manoj Y B Madhavi Reddy K
author_sort	Jessy Mol K K
title	Validation of Molecular Markers Genetically Linked to S-Cytoplasm and Restoration-of-fertility (Rf) Loci in Hot Pepper (Capsicum annuum L.)
title_short	Validation of Molecular Markers Genetically Linked to S-Cytoplasm and Restoration-of-fertility (Rf) Loci in Hot Pepper (Capsicum annuum L.)
title_full	Validation of Molecular Markers Genetically Linked to S-Cytoplasm and Restoration-of-fertility (Rf) Loci in Hot Pepper (Capsicum annuum L.)
title_fullStr	Validation of Molecular Markers Genetically Linked to S-Cytoplasm and Restoration-of-fertility (Rf) Loci in Hot Pepper (Capsicum annuum L.)
title_full_unstemmed	Validation of Molecular Markers Genetically Linked to S-Cytoplasm and Restoration-of-fertility (Rf) Loci in Hot Pepper (Capsicum annuum L.)
title_sort	validation of molecular markers genetically linked to s-cytoplasm and restoration-of-fertility (rf) loci in hot pepper (capsicum annuum l.)
publisher	Society for Promotion of Horticulture - Indian Institute of Horticultural Research
publishDate	2020
url	https://doaj.org/article/3f4a47056433400a9b1e60afc4ecf282
work_keys_str_mv	AT jessymolkk validationofmolecularmarkersgeneticallylinkedtoscytoplasmandrestorationoffertilityrflociinhotpeppercapsicumannuuml AT lakshmanareddydc validationofmolecularmarkersgeneticallylinkedtoscytoplasmandrestorationoffertilityrflociinhotpeppercapsicumannuuml AT manojyb validationofmolecularmarkersgeneticallylinkedtoscytoplasmandrestorationoffertilityrflociinhotpeppercapsicumannuuml AT madhavireddyk validationofmolecularmarkersgeneticallylinkedtoscytoplasmandrestorationoffertilityrflociinhotpeppercapsicumannuuml
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Validation of Molecular Markers Genetically Linked to S-Cytoplasm and Restoration-of-fertility (Rf) Loci in Hot Pepper (Capsicum annuum L.)

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