Genome-wide identification and expression pattern analysis of lipoxygenase gene family in banana

Abstract The LOX genes have been identified and characterized in many plant species, but studies on the banana LOX genes are very limited. In this study, we respectively identified 18 MaLOX, 11 MbLOX, and 12 MiLOX genes from the Musa acuminata, M. balbisiana and M. itinerans genome data, investigate...

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Autores principales:	Fan Liu, Hua Li, Junwei Wu, Bin Wang, Na Tian, Jiapeng Liu, Xueli Sun, Huan Wu, Yuji Huang, Peitao Lü, Chunzhen Cheng
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/3f55db572c514d0fba47a746798cf0d1
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spelling	oai:doaj.org-article:3f55db572c514d0fba47a746798cf0d12021-12-02T15:36:30ZGenome-wide identification and expression pattern analysis of lipoxygenase gene family in banana10.1038/s41598-021-89211-62045-2322https://doaj.org/article/3f55db572c514d0fba47a746798cf0d12021-05-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-89211-6https://doaj.org/toc/2045-2322Abstract The LOX genes have been identified and characterized in many plant species, but studies on the banana LOX genes are very limited. In this study, we respectively identified 18 MaLOX, 11 MbLOX, and 12 MiLOX genes from the Musa acuminata, M. balbisiana and M. itinerans genome data, investigated their gene structures and characterized the physicochemical properties of their encoded proteins. Banana LOXs showed a preference for using and ending with G/C and their encoded proteins can be classified into 9-LOX, Type I 13-LOX and Type II 13-LOX subfamilies. The expansion of the MaLOXs might result from the combined actions of genome-wide, tandem, and segmental duplications. However, tandem and segmental duplications contribute to the expansion of MbLOXs. Transcriptome data based gene expression analysis showed that MaLOX1, 4, and 7 were highly expressed in fruit and their expression levels were significantly regulated by ethylene. And 11, 12 and 7 MaLOXs were found to be low temperature-, high temperature-, and Fusarium oxysporum f. sp. Cubense tropical race 4 (FocTR4)-responsive, respectively. MaLOX8, 9 and 13 are responsive to all the three stresses, MaLOX4 and MaLOX12 are high temperature- and FocTR4-responsive; MaLOX6 and MaLOX17 are significantly induced by low temperature and FocTR4; and the expression of MaLOX7 and MaLOX16 are only affected by high temperature. Quantitative real-time PCR (qRT-PCR) analysis revealed that the expression levels of several MaLOXs are regulated by MeJA and FocTR4, indicating that they can increase the resistance of banana by regulating the JA pathway. Additionally, the weighted gene co-expression network analysis (WGCNA) of MaLOXs revealed 3 models respectively for 5 (MaLOX7-11), 3 (MaLOX6, 13, and 17), and 1 (MaLOX12) MaLOX genes. Our findings can provide valuable information for the characterization, evolution, diversity and functionality of MaLOX, MbLOX and MiLOX genes and are helpful for understanding the roles of LOXs in banana growth and development and adaptations to different stresses.Fan LiuHua LiJunwei WuBin WangNa TianJiapeng LiuXueli SunHuan WuYuji HuangPeitao LüChunzhen ChengNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-19 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Fan Liu Hua Li Junwei Wu Bin Wang Na Tian Jiapeng Liu Xueli Sun Huan Wu Yuji Huang Peitao Lü Chunzhen Cheng Genome-wide identification and expression pattern analysis of lipoxygenase gene family in banana
description	Abstract The LOX genes have been identified and characterized in many plant species, but studies on the banana LOX genes are very limited. In this study, we respectively identified 18 MaLOX, 11 MbLOX, and 12 MiLOX genes from the Musa acuminata, M. balbisiana and M. itinerans genome data, investigated their gene structures and characterized the physicochemical properties of their encoded proteins. Banana LOXs showed a preference for using and ending with G/C and their encoded proteins can be classified into 9-LOX, Type I 13-LOX and Type II 13-LOX subfamilies. The expansion of the MaLOXs might result from the combined actions of genome-wide, tandem, and segmental duplications. However, tandem and segmental duplications contribute to the expansion of MbLOXs. Transcriptome data based gene expression analysis showed that MaLOX1, 4, and 7 were highly expressed in fruit and their expression levels were significantly regulated by ethylene. And 11, 12 and 7 MaLOXs were found to be low temperature-, high temperature-, and Fusarium oxysporum f. sp. Cubense tropical race 4 (FocTR4)-responsive, respectively. MaLOX8, 9 and 13 are responsive to all the three stresses, MaLOX4 and MaLOX12 are high temperature- and FocTR4-responsive; MaLOX6 and MaLOX17 are significantly induced by low temperature and FocTR4; and the expression of MaLOX7 and MaLOX16 are only affected by high temperature. Quantitative real-time PCR (qRT-PCR) analysis revealed that the expression levels of several MaLOXs are regulated by MeJA and FocTR4, indicating that they can increase the resistance of banana by regulating the JA pathway. Additionally, the weighted gene co-expression network analysis (WGCNA) of MaLOXs revealed 3 models respectively for 5 (MaLOX7-11), 3 (MaLOX6, 13, and 17), and 1 (MaLOX12) MaLOX genes. Our findings can provide valuable information for the characterization, evolution, diversity and functionality of MaLOX, MbLOX and MiLOX genes and are helpful for understanding the roles of LOXs in banana growth and development and adaptations to different stresses.
format	article
author	Fan Liu Hua Li Junwei Wu Bin Wang Na Tian Jiapeng Liu Xueli Sun Huan Wu Yuji Huang Peitao Lü Chunzhen Cheng
author_facet	Fan Liu Hua Li Junwei Wu Bin Wang Na Tian Jiapeng Liu Xueli Sun Huan Wu Yuji Huang Peitao Lü Chunzhen Cheng
author_sort	Fan Liu
title	Genome-wide identification and expression pattern analysis of lipoxygenase gene family in banana
title_short	Genome-wide identification and expression pattern analysis of lipoxygenase gene family in banana
title_full	Genome-wide identification and expression pattern analysis of lipoxygenase gene family in banana
title_fullStr	Genome-wide identification and expression pattern analysis of lipoxygenase gene family in banana
title_full_unstemmed	Genome-wide identification and expression pattern analysis of lipoxygenase gene family in banana
title_sort	genome-wide identification and expression pattern analysis of lipoxygenase gene family in banana
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/3f55db572c514d0fba47a746798cf0d1
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Genome-wide identification and expression pattern analysis of lipoxygenase gene family in banana

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