Fast live-cell conventional fluorophore nanoscopy with ImageJ through super-resolution radial fluctuations

Super-resolution fluorescent imaging typically makes use of intense phototoxic illumination. Here the authors achieve live-cell super-resolution imaging using low-illumination standard microscopes with the aid of a new analytical approach called Super-Resolution Radial Fluctuations (SRRF), provided...

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Autores principales:	Nils Gustafsson, Siân Culley, George Ashdown, Dylan M. Owen, Pedro Matos Pereira, Ricardo Henriques
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2016
Materias:	Science Q
Acceso en línea:	https://doaj.org/article/3f58ca8cc60e4b8db69d99381d87fa14
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id	oai:doaj.org-article:3f58ca8cc60e4b8db69d99381d87fa14
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spelling	oai:doaj.org-article:3f58ca8cc60e4b8db69d99381d87fa142021-12-02T16:49:41ZFast live-cell conventional fluorophore nanoscopy with ImageJ through super-resolution radial fluctuations10.1038/ncomms124712041-1723https://doaj.org/article/3f58ca8cc60e4b8db69d99381d87fa142016-08-01T00:00:00Zhttps://doi.org/10.1038/ncomms12471https://doaj.org/toc/2041-1723Super-resolution fluorescent imaging typically makes use of intense phototoxic illumination. Here the authors achieve live-cell super-resolution imaging using low-illumination standard microscopes with the aid of a new analytical approach called Super-Resolution Radial Fluctuations (SRRF), provided as an ImageJ plugin.Nils GustafssonSiân CulleyGeorge AshdownDylan M. OwenPedro Matos PereiraRicardo HenriquesNature PortfolioarticleScienceQENNature Communications, Vol 7, Iss 1, Pp 1-9 (2016)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q Nils Gustafsson Siân Culley George Ashdown Dylan M. Owen Pedro Matos Pereira Ricardo Henriques Fast live-cell conventional fluorophore nanoscopy with ImageJ through super-resolution radial fluctuations
description	Super-resolution fluorescent imaging typically makes use of intense phototoxic illumination. Here the authors achieve live-cell super-resolution imaging using low-illumination standard microscopes with the aid of a new analytical approach called Super-Resolution Radial Fluctuations (SRRF), provided as an ImageJ plugin.
format	article
author	Nils Gustafsson Siân Culley George Ashdown Dylan M. Owen Pedro Matos Pereira Ricardo Henriques
author_facet	Nils Gustafsson Siân Culley George Ashdown Dylan M. Owen Pedro Matos Pereira Ricardo Henriques
author_sort	Nils Gustafsson
title	Fast live-cell conventional fluorophore nanoscopy with ImageJ through super-resolution radial fluctuations
title_short	Fast live-cell conventional fluorophore nanoscopy with ImageJ through super-resolution radial fluctuations
title_full	Fast live-cell conventional fluorophore nanoscopy with ImageJ through super-resolution radial fluctuations
title_fullStr	Fast live-cell conventional fluorophore nanoscopy with ImageJ through super-resolution radial fluctuations
title_full_unstemmed	Fast live-cell conventional fluorophore nanoscopy with ImageJ through super-resolution radial fluctuations
title_sort	fast live-cell conventional fluorophore nanoscopy with imagej through super-resolution radial fluctuations
publisher	Nature Portfolio
publishDate	2016
url	https://doaj.org/article/3f58ca8cc60e4b8db69d99381d87fa14
work_keys_str_mv	AT nilsgustafsson fastlivecellconventionalfluorophorenanoscopywithimagejthroughsuperresolutionradialfluctuations AT sianculley fastlivecellconventionalfluorophorenanoscopywithimagejthroughsuperresolutionradialfluctuations AT georgeashdown fastlivecellconventionalfluorophorenanoscopywithimagejthroughsuperresolutionradialfluctuations AT dylanmowen fastlivecellconventionalfluorophorenanoscopywithimagejthroughsuperresolutionradialfluctuations AT pedromatospereira fastlivecellconventionalfluorophorenanoscopywithimagejthroughsuperresolutionradialfluctuations AT ricardohenriques fastlivecellconventionalfluorophorenanoscopywithimagejthroughsuperresolutionradialfluctuations
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Fast live-cell conventional fluorophore nanoscopy with ImageJ through super-resolution radial fluctuations

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