Arginine–glycine–aspartic acid–polyethylene glycol–polyamidoamine dendrimer conjugate improves liver-cell aggregation and function in 3-D spheroid culture

Arginine–glycine–aspartic acid–polyethylene glycol–polyamidoamine dendrimer conjugate improves liver-cell aggregation and function in 3-D spheroid culture

Zhanfei Chen,1,* Fen Lian,1,* Xiaoqian Wang,1 Yanling Chen,1,2 Nanhong Tang1,2 1Fujian Institute of Hepatobiliary Surgery, Fujian Medical University Union Hospital, 2Key Laboratory of Ministry of Education for Gastrointestinal Cancer, Research Center for Molecular Medicine, Fujian Medical Universit...

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Autores principales: Chen Z, Lian F, Wang X, Chen Y, Tang N
Formato: article
Lenguaje:EN
Publicado: Dove Medical Press 2016
Materias:
dendrimer
Arg-Gly-Asp (RGD) peptide
hepatic cell
spheroid culture
ammonia metabolism
Medicine (General)
R5-920
Acceso en línea:https://doaj.org/article/3f68aa1c3c8741ffbbd14d21a58a209f
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spelling oai:doaj.org-article:3f68aa1c3c8741ffbbd14d21a58a209f2021-12-02T00:57:28ZArginine–glycine–aspartic acid–polyethylene glycol–polyamidoamine dendrimer conjugate improves liver-cell aggregation and function in 3-D spheroid culture1178-2013https://doaj.org/article/3f68aa1c3c8741ffbbd14d21a58a209f2016-08-01T00:00:00Zhttps://www.dovepress.com/argininendashglycinendashaspartic-acidndashpolyethylene-glycolndashpol-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Zhanfei Chen,1,* Fen Lian,1,* Xiaoqian Wang,1 Yanling Chen,1,2 Nanhong Tang1,2 1Fujian Institute of Hepatobiliary Surgery, Fujian Medical University Union Hospital, 2Key Laboratory of Ministry of Education for Gastrointestinal Cancer, Research Center for Molecular Medicine, Fujian Medical University, Fuzhou, People’s Republic of China *These authors contributed equally to this work Abstract: The polyamidoamine (PAMAM) dendrimer, a type of macromolecule material, has been used in spheroidal cell culture and drug delivery in recent years. However, PAMAM is not involved in the study of hepatic cell-spheroid culture or its biological activity, particularly in detoxification function. Here, we constructed a PAMAM-dendrimer conjugate decorated by an integrin ligand: arginine–glycine–aspartic acid (RGD) peptide. Our studies demonstrate that RGD–polyethylene glycol (PEG)–PAMAM conjugates can promote singly floating hepatic cells to aggregate together in a sphere-like growth with a weak reactive oxygen species. Moreover, RGD-PEG-PAMAM conjugates can activate the AKT–MAPK pathway in hepatic cells to promote cell proliferation and improve basic function and ammonia metabolism. Together, our data support the hepatocyte sphere treated by RGD-PEG-PAMAM conjugates as a potential source of hepatic cells for a biological artificial liver system. Keywords: dendrimer, arginine–glycine–aspartic acid (RGD), liver cell, spheroid culture, ammonia metabolismChen ZLian FWang XChen YTang NDove Medical PressarticledendrimerArg-Gly-Asp (RGD) peptidehepatic cellspheroid cultureammonia metabolismMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 11, Pp 4247-4259 (2016)
institution DOAJ
collection DOAJ
language EN
topic dendrimer
Arg-Gly-Asp (RGD) peptide
hepatic cell
spheroid culture
ammonia metabolism
Medicine (General)
R5-920
spellingShingle dendrimer
Arg-Gly-Asp (RGD) peptide
hepatic cell
spheroid culture
ammonia metabolism
Medicine (General)
R5-920
Chen Z
Lian F
Wang X
Chen Y
Tang N
Arginine–glycine–aspartic acid–polyethylene glycol–polyamidoamine dendrimer conjugate improves liver-cell aggregation and function in 3-D spheroid culture
description Zhanfei Chen,1,* Fen Lian,1,* Xiaoqian Wang,1 Yanling Chen,1,2 Nanhong Tang1,2 1Fujian Institute of Hepatobiliary Surgery, Fujian Medical University Union Hospital, 2Key Laboratory of Ministry of Education for Gastrointestinal Cancer, Research Center for Molecular Medicine, Fujian Medical University, Fuzhou, People’s Republic of China *These authors contributed equally to this work Abstract: The polyamidoamine (PAMAM) dendrimer, a type of macromolecule material, has been used in spheroidal cell culture and drug delivery in recent years. However, PAMAM is not involved in the study of hepatic cell-spheroid culture or its biological activity, particularly in detoxification function. Here, we constructed a PAMAM-dendrimer conjugate decorated by an integrin ligand: arginine–glycine–aspartic acid (RGD) peptide. Our studies demonstrate that RGD–polyethylene glycol (PEG)–PAMAM conjugates can promote singly floating hepatic cells to aggregate together in a sphere-like growth with a weak reactive oxygen species. Moreover, RGD-PEG-PAMAM conjugates can activate the AKT–MAPK pathway in hepatic cells to promote cell proliferation and improve basic function and ammonia metabolism. Together, our data support the hepatocyte sphere treated by RGD-PEG-PAMAM conjugates as a potential source of hepatic cells for a biological artificial liver system. Keywords: dendrimer, arginine–glycine–aspartic acid (RGD), liver cell, spheroid culture, ammonia metabolism
format article
author Chen Z
Lian F
Wang X
Chen Y
Tang N
author_facet Chen Z
Lian F
Wang X
Chen Y
Tang N
author_sort Chen Z
title Arginine–glycine–aspartic acid–polyethylene glycol–polyamidoamine dendrimer conjugate improves liver-cell aggregation and function in 3-D spheroid culture
title_short Arginine–glycine–aspartic acid–polyethylene glycol–polyamidoamine dendrimer conjugate improves liver-cell aggregation and function in 3-D spheroid culture
title_full Arginine–glycine–aspartic acid–polyethylene glycol–polyamidoamine dendrimer conjugate improves liver-cell aggregation and function in 3-D spheroid culture
title_fullStr Arginine–glycine–aspartic acid–polyethylene glycol–polyamidoamine dendrimer conjugate improves liver-cell aggregation and function in 3-D spheroid culture
title_full_unstemmed Arginine–glycine–aspartic acid–polyethylene glycol–polyamidoamine dendrimer conjugate improves liver-cell aggregation and function in 3-D spheroid culture
title_sort arginine–glycine–aspartic acid–polyethylene glycol–polyamidoamine dendrimer conjugate improves liver-cell aggregation and function in 3-d spheroid culture
publisher Dove Medical Press
publishDate 2016
url https://doaj.org/article/3f68aa1c3c8741ffbbd14d21a58a209f
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AT lianf argininendashglycinendashasparticacidndashpolyethyleneglycolndashpolyamidoaminedendrimerconjugateimproveslivercellaggregationandfunctionin3dspheroidculture
AT wangx argininendashglycinendashasparticacidndashpolyethyleneglycolndashpolyamidoaminedendrimerconjugateimproveslivercellaggregationandfunctionin3dspheroidculture
AT cheny argininendashglycinendashasparticacidndashpolyethyleneglycolndashpolyamidoaminedendrimerconjugateimproveslivercellaggregationandfunctionin3dspheroidculture
AT tangn argininendashglycinendashasparticacidndashpolyethyleneglycolndashpolyamidoaminedendrimerconjugateimproveslivercellaggregationandfunctionin3dspheroidculture
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