Variation of Neisseria gonorrhoeae lipooligosaccharide directs dendritic cell-induced T helper responses.

Gonorrhea is one of the most prevalent sexually transmitted diseases in the world. A naturally occurring variation of the terminal carbohydrates on the lipooligosaccharide (LOS) molecule correlates with altered disease states. Here, we investigated the interaction of different stable gonoccocal LOS...

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Autores principales:	Sandra J van Vliet, Liana Steeghs, Sven C M Bruijns, Medi M Vaezirad, Christian Snijders Blok, Jésus A Arenas Busto, Marcel Deken, Jos P M van Putten, Yvette van Kooyk
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2009
Materias:	Immunologic diseases. Allergy RC581-607 Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/3f6c8765689b42e58e6c4d5ffe676cf6
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spelling	oai:doaj.org-article:3f6c8765689b42e58e6c4d5ffe676cf62021-11-25T05:48:35ZVariation of Neisseria gonorrhoeae lipooligosaccharide directs dendritic cell-induced T helper responses.1553-73661553-737410.1371/journal.ppat.1000625https://doaj.org/article/3f6c8765689b42e58e6c4d5ffe676cf62009-10-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/19834553/pdf/?tool=EBIhttps://doaj.org/toc/1553-7366https://doaj.org/toc/1553-7374Gonorrhea is one of the most prevalent sexually transmitted diseases in the world. A naturally occurring variation of the terminal carbohydrates on the lipooligosaccharide (LOS) molecule correlates with altered disease states. Here, we investigated the interaction of different stable gonoccocal LOS phenotypes with human dendritic cells and demonstrate that each variant targets a different set of receptors on the dendritic cell, including the C-type lectins MGL and DC-SIGN. Neisseria gonorrhoeae LOS phenotype C constitutes the first bacterial ligand to be described for the human C-type lectin receptor MGL. Both MGL and DC-SIGN are locally expressed at the male and female genital area, the primary site of N. gonorrhoeae infection. We show that targeting of different C-type lectins with the N. gonorrhoeae LOS variants results in alterations in dendritic cell cytokine secretion profiles and the induction of distinct adaptive CD4(+) T helper responses. Whereas N. gonorrhoeae variant A with a terminal N-acetylglucosamine on its LOS was recognized by DC-SIGN and induced significantly more IL-10 production, phenotype C, carrying a terminal N-acetylgalactosamine, primarily interacted with MGL and skewed immunity towards the T helper 2 lineage. Together, our results indicate that N. gonorrhoeae LOS variation allows for selective manipulation of dendritic cell function, thereby shifting subsequent immune responses in favor of bacterial survival.Sandra J van VlietLiana SteeghsSven C M BruijnsMedi M VaeziradChristian Snijders BlokJésus A Arenas BustoMarcel DekenJos P M van PuttenYvette van KooykPublic Library of Science (PLoS)articleImmunologic diseases. AllergyRC581-607Biology (General)QH301-705.5ENPLoS Pathogens, Vol 5, Iss 10, p e1000625 (2009)
institution	DOAJ
collection	DOAJ
language	EN
topic	Immunologic diseases. Allergy RC581-607 Biology (General) QH301-705.5
spellingShingle	Immunologic diseases. Allergy RC581-607 Biology (General) QH301-705.5 Sandra J van Vliet Liana Steeghs Sven C M Bruijns Medi M Vaezirad Christian Snijders Blok Jésus A Arenas Busto Marcel Deken Jos P M van Putten Yvette van Kooyk Variation of Neisseria gonorrhoeae lipooligosaccharide directs dendritic cell-induced T helper responses.
description	Gonorrhea is one of the most prevalent sexually transmitted diseases in the world. A naturally occurring variation of the terminal carbohydrates on the lipooligosaccharide (LOS) molecule correlates with altered disease states. Here, we investigated the interaction of different stable gonoccocal LOS phenotypes with human dendritic cells and demonstrate that each variant targets a different set of receptors on the dendritic cell, including the C-type lectins MGL and DC-SIGN. Neisseria gonorrhoeae LOS phenotype C constitutes the first bacterial ligand to be described for the human C-type lectin receptor MGL. Both MGL and DC-SIGN are locally expressed at the male and female genital area, the primary site of N. gonorrhoeae infection. We show that targeting of different C-type lectins with the N. gonorrhoeae LOS variants results in alterations in dendritic cell cytokine secretion profiles and the induction of distinct adaptive CD4(+) T helper responses. Whereas N. gonorrhoeae variant A with a terminal N-acetylglucosamine on its LOS was recognized by DC-SIGN and induced significantly more IL-10 production, phenotype C, carrying a terminal N-acetylgalactosamine, primarily interacted with MGL and skewed immunity towards the T helper 2 lineage. Together, our results indicate that N. gonorrhoeae LOS variation allows for selective manipulation of dendritic cell function, thereby shifting subsequent immune responses in favor of bacterial survival.
format	article
author	Sandra J van Vliet Liana Steeghs Sven C M Bruijns Medi M Vaezirad Christian Snijders Blok Jésus A Arenas Busto Marcel Deken Jos P M van Putten Yvette van Kooyk
author_facet	Sandra J van Vliet Liana Steeghs Sven C M Bruijns Medi M Vaezirad Christian Snijders Blok Jésus A Arenas Busto Marcel Deken Jos P M van Putten Yvette van Kooyk
author_sort	Sandra J van Vliet
title	Variation of Neisseria gonorrhoeae lipooligosaccharide directs dendritic cell-induced T helper responses.
title_short	Variation of Neisseria gonorrhoeae lipooligosaccharide directs dendritic cell-induced T helper responses.
title_full	Variation of Neisseria gonorrhoeae lipooligosaccharide directs dendritic cell-induced T helper responses.
title_fullStr	Variation of Neisseria gonorrhoeae lipooligosaccharide directs dendritic cell-induced T helper responses.
title_full_unstemmed	Variation of Neisseria gonorrhoeae lipooligosaccharide directs dendritic cell-induced T helper responses.
title_sort	variation of neisseria gonorrhoeae lipooligosaccharide directs dendritic cell-induced t helper responses.
publisher	Public Library of Science (PLoS)
publishDate	2009
url	https://doaj.org/article/3f6c8765689b42e58e6c4d5ffe676cf6
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Variation of Neisseria gonorrhoeae lipooligosaccharide directs dendritic cell-induced T helper responses.

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