Functional characterization of detergent-decellularized equine tendon extracellular matrix for tissue engineering applications.

Natural extracellular matrix provides a number of distinct advantages for engineering replacement orthopedic tissue due to its intrinsic functional properties. The goal of this study was to optimize a biologically derived scaffold for tendon tissue engineering using equine flexor digitorum superfici...

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Auteurs principaux:	Daniel W Youngstrom, Jennifer G Barrett, Rod R Jose, David L Kaplan
Format:	article
Langue:	EN
Publié:	Public Library of Science (PLoS) 2013
Sujets:	Medicine R Science Q
Accès en ligne:	https://doaj.org/article/3f6d4a7f5cd84f40a9f63f76a085fcbf
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spelling	oai:doaj.org-article:3f6d4a7f5cd84f40a9f63f76a085fcbf2021-11-18T07:44:15ZFunctional characterization of detergent-decellularized equine tendon extracellular matrix for tissue engineering applications.1932-620310.1371/journal.pone.0064151https://doaj.org/article/3f6d4a7f5cd84f40a9f63f76a085fcbf2013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23724028/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203Natural extracellular matrix provides a number of distinct advantages for engineering replacement orthopedic tissue due to its intrinsic functional properties. The goal of this study was to optimize a biologically derived scaffold for tendon tissue engineering using equine flexor digitorum superficialis tendons. We investigated changes in scaffold composition and ultrastructure in response to several mechanical, detergent and enzymatic decellularization protocols using microscopic techniques and a panel of biochemical assays to evaluate total protein, collagen, glycosaminoglycan, and deoxyribonucleic acid content. Biocompatibility was also assessed with static mesenchymal stem cell (MSC) culture. Implementation of a combination of freeze/thaw cycles, incubation in 2% sodium dodecyl sulfate (SDS), trypsinization, treatment with DNase-I, and ethanol sterilization produced a non-cytotoxic biomaterial free of appreciable residual cellular debris with no significant modification of biomechanical properties. These decellularized tendon scaffolds (DTS) are suitable for complex tissue engineering applications, as they provide a clean slate for cell culture while maintaining native three-dimensional architecture.Daniel W YoungstromJennifer G BarrettRod R JoseDavid L KaplanPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 5, p e64151 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Daniel W Youngstrom Jennifer G Barrett Rod R Jose David L Kaplan Functional characterization of detergent-decellularized equine tendon extracellular matrix for tissue engineering applications.
description	Natural extracellular matrix provides a number of distinct advantages for engineering replacement orthopedic tissue due to its intrinsic functional properties. The goal of this study was to optimize a biologically derived scaffold for tendon tissue engineering using equine flexor digitorum superficialis tendons. We investigated changes in scaffold composition and ultrastructure in response to several mechanical, detergent and enzymatic decellularization protocols using microscopic techniques and a panel of biochemical assays to evaluate total protein, collagen, glycosaminoglycan, and deoxyribonucleic acid content. Biocompatibility was also assessed with static mesenchymal stem cell (MSC) culture. Implementation of a combination of freeze/thaw cycles, incubation in 2% sodium dodecyl sulfate (SDS), trypsinization, treatment with DNase-I, and ethanol sterilization produced a non-cytotoxic biomaterial free of appreciable residual cellular debris with no significant modification of biomechanical properties. These decellularized tendon scaffolds (DTS) are suitable for complex tissue engineering applications, as they provide a clean slate for cell culture while maintaining native three-dimensional architecture.
format	article
author	Daniel W Youngstrom Jennifer G Barrett Rod R Jose David L Kaplan
author_facet	Daniel W Youngstrom Jennifer G Barrett Rod R Jose David L Kaplan
author_sort	Daniel W Youngstrom
title	Functional characterization of detergent-decellularized equine tendon extracellular matrix for tissue engineering applications.
title_short	Functional characterization of detergent-decellularized equine tendon extracellular matrix for tissue engineering applications.
title_full	Functional characterization of detergent-decellularized equine tendon extracellular matrix for tissue engineering applications.
title_fullStr	Functional characterization of detergent-decellularized equine tendon extracellular matrix for tissue engineering applications.
title_full_unstemmed	Functional characterization of detergent-decellularized equine tendon extracellular matrix for tissue engineering applications.
title_sort	functional characterization of detergent-decellularized equine tendon extracellular matrix for tissue engineering applications.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/3f6d4a7f5cd84f40a9f63f76a085fcbf
work_keys_str_mv	AT danielwyoungstrom functionalcharacterizationofdetergentdecellularizedequinetendonextracellularmatrixfortissueengineeringapplications AT jennifergbarrett functionalcharacterizationofdetergentdecellularizedequinetendonextracellularmatrixfortissueengineeringapplications AT rodrjose functionalcharacterizationofdetergentdecellularizedequinetendonextracellularmatrixfortissueengineeringapplications AT davidlkaplan functionalcharacterizationofdetergentdecellularizedequinetendonextracellularmatrixfortissueengineeringapplications
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Functional characterization of detergent-decellularized equine tendon extracellular matrix for tissue engineering applications.

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