Functional characterization of detergent-decellularized equine tendon extracellular matrix for tissue engineering applications.

Natural extracellular matrix provides a number of distinct advantages for engineering replacement orthopedic tissue due to its intrinsic functional properties. The goal of this study was to optimize a biologically derived scaffold for tendon tissue engineering using equine flexor digitorum superfici...

Descripción completa

Guardado en:
Detalles Bibliográficos
Autores principales: Daniel W Youngstrom, Jennifer G Barrett, Rod R Jose, David L Kaplan
Formato: article
Lenguaje:EN
Publicado: Public Library of Science (PLoS) 2013
Materias:
R
Q
Acceso en línea:https://doaj.org/article/3f6d4a7f5cd84f40a9f63f76a085fcbf
Etiquetas: Agregar Etiqueta
Sin Etiquetas, Sea el primero en etiquetar este registro!
id oai:doaj.org-article:3f6d4a7f5cd84f40a9f63f76a085fcbf
record_format dspace
spelling oai:doaj.org-article:3f6d4a7f5cd84f40a9f63f76a085fcbf2021-11-18T07:44:15ZFunctional characterization of detergent-decellularized equine tendon extracellular matrix for tissue engineering applications.1932-620310.1371/journal.pone.0064151https://doaj.org/article/3f6d4a7f5cd84f40a9f63f76a085fcbf2013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23724028/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203Natural extracellular matrix provides a number of distinct advantages for engineering replacement orthopedic tissue due to its intrinsic functional properties. The goal of this study was to optimize a biologically derived scaffold for tendon tissue engineering using equine flexor digitorum superficialis tendons. We investigated changes in scaffold composition and ultrastructure in response to several mechanical, detergent and enzymatic decellularization protocols using microscopic techniques and a panel of biochemical assays to evaluate total protein, collagen, glycosaminoglycan, and deoxyribonucleic acid content. Biocompatibility was also assessed with static mesenchymal stem cell (MSC) culture. Implementation of a combination of freeze/thaw cycles, incubation in 2% sodium dodecyl sulfate (SDS), trypsinization, treatment with DNase-I, and ethanol sterilization produced a non-cytotoxic biomaterial free of appreciable residual cellular debris with no significant modification of biomechanical properties. These decellularized tendon scaffolds (DTS) are suitable for complex tissue engineering applications, as they provide a clean slate for cell culture while maintaining native three-dimensional architecture.Daniel W YoungstromJennifer G BarrettRod R JoseDavid L KaplanPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 5, p e64151 (2013)
institution DOAJ
collection DOAJ
language EN
topic Medicine
R
Science
Q
spellingShingle Medicine
R
Science
Q
Daniel W Youngstrom
Jennifer G Barrett
Rod R Jose
David L Kaplan
Functional characterization of detergent-decellularized equine tendon extracellular matrix for tissue engineering applications.
description Natural extracellular matrix provides a number of distinct advantages for engineering replacement orthopedic tissue due to its intrinsic functional properties. The goal of this study was to optimize a biologically derived scaffold for tendon tissue engineering using equine flexor digitorum superficialis tendons. We investigated changes in scaffold composition and ultrastructure in response to several mechanical, detergent and enzymatic decellularization protocols using microscopic techniques and a panel of biochemical assays to evaluate total protein, collagen, glycosaminoglycan, and deoxyribonucleic acid content. Biocompatibility was also assessed with static mesenchymal stem cell (MSC) culture. Implementation of a combination of freeze/thaw cycles, incubation in 2% sodium dodecyl sulfate (SDS), trypsinization, treatment with DNase-I, and ethanol sterilization produced a non-cytotoxic biomaterial free of appreciable residual cellular debris with no significant modification of biomechanical properties. These decellularized tendon scaffolds (DTS) are suitable for complex tissue engineering applications, as they provide a clean slate for cell culture while maintaining native three-dimensional architecture.
format article
author Daniel W Youngstrom
Jennifer G Barrett
Rod R Jose
David L Kaplan
author_facet Daniel W Youngstrom
Jennifer G Barrett
Rod R Jose
David L Kaplan
author_sort Daniel W Youngstrom
title Functional characterization of detergent-decellularized equine tendon extracellular matrix for tissue engineering applications.
title_short Functional characterization of detergent-decellularized equine tendon extracellular matrix for tissue engineering applications.
title_full Functional characterization of detergent-decellularized equine tendon extracellular matrix for tissue engineering applications.
title_fullStr Functional characterization of detergent-decellularized equine tendon extracellular matrix for tissue engineering applications.
title_full_unstemmed Functional characterization of detergent-decellularized equine tendon extracellular matrix for tissue engineering applications.
title_sort functional characterization of detergent-decellularized equine tendon extracellular matrix for tissue engineering applications.
publisher Public Library of Science (PLoS)
publishDate 2013
url https://doaj.org/article/3f6d4a7f5cd84f40a9f63f76a085fcbf
work_keys_str_mv AT danielwyoungstrom functionalcharacterizationofdetergentdecellularizedequinetendonextracellularmatrixfortissueengineeringapplications
AT jennifergbarrett functionalcharacterizationofdetergentdecellularizedequinetendonextracellularmatrixfortissueengineeringapplications
AT rodrjose functionalcharacterizationofdetergentdecellularizedequinetendonextracellularmatrixfortissueengineeringapplications
AT davidlkaplan functionalcharacterizationofdetergentdecellularizedequinetendonextracellularmatrixfortissueengineeringapplications
_version_ 1718423030816833536